LOCUS       sequence-100230-        2039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of nuclear AMPK biosensor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: Nuc-ABKAR
FEATURES             Location/Qualifiers
     source          1..2039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(8..10,11..13,14..724)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1247..1981
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     CDS             1988..2008
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggatcccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
       61 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      121 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      181 gcccaccctc gtgaccaccc tgagctgggg cgtgcagtgc ttcgcccgct accccgacca
      241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      421 ggggcacaag ctggagtaca acgccatcca cggcaacgtc tatatcaccg ccgacaagca
      481 gaagaacggc atcaaggcca acttcggcct caactgcaac atcgaggacg gcagcgtgca
      541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      601 caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca
      661 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
      721 caagcgcatg cataagtttt ctcaagaaca gatcggcgaa aacattgtgt gcagggtcat
      781 ttgtaccacg ggtcaaattc ccatccgaga tttgtcagct gatatttcac aagtgcttaa
      841 ggaaaaacga tccataaaga aagtttggac atttggtaga aacccagcct gtgactatca
      901 tttaggaaac atttcaagac tgtcaaataa gcatttccaa atactactag gagaagacgg
      961 taacctttta ttgaatgaca tttccactaa tgggacctgg ttaaatgggc aaaaagtcga
     1021 gaagaacagc aatcagttac tgtctcaagg tgatgaaata accgttggtg taggcgtgga
     1081 atcagatatt ttatctctgg tcattttcat aaacgacaaa tttaagcagt gcctcgagca
     1141 gaacaaagtt gatcgctctg caggtaagcc aggcagcggc gagggcagca ccaagatgcg
     1201 tcgtgtcgcc accctggtgg acctcggcac cggcggcagc gagctcatgg gcggcgtgca
     1261 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1321 caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     1381 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1441 caagggaggt accggtggat ctatggtgag caagggcgag gagctgttca ccggggtggt
     1501 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1561 gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa
     1621 gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggccttc agtgcttcgc
     1681 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1741 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1801 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1861 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1921 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     1981 ggaattcccc aaaaagaaga gaaaggtgga agacgcataa nntagagggc cntnnnnna
//
