LOCUS       sequence-100233-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to purify human ITPA from bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Porta J, Kolar C, Kozmin SG, Pavlov YI, Borgstahl GE
  TITLE     Structure of the orthorhombic form of human inosine triphosphate 
            pyrophosphatase.
  JOURNAL   Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt 
            11):1076-81. Epub 2006 Oct 25.
  PUBMED    17077483
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr Sect F Struct Biol Cryst Commun."; date: "2006-11-1"; 
            volume: "62(Pt 11)"; pages: "1076-81. Epub 2006 Oct 25"
COMMENT     Sequence Label: pET15b-ITPA
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..103
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(771..789)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      785..832
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ccctctagaa aataattttg gtttaacttt aagaaggaga tataccatgg gcagcagcca
       61 tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgg cggcctcatt
      121 ggtggggaag aagatcgtgt ttgtaacggg gaacgccaag aagctggagg aggtcgttca
      181 gattctagga gataagtttc catgcacttt ggtggcacag aaaattgacc tgccggagta
      241 ccagggggag ccggatgaga tttccataca gaaatgtcag gaggcagttc gccaggtaca
      301 ggggcccgtg ctggttgagg acacttgtct gtgcttcaat gcccttggag ggctccccgg
      361 cccctacata aagtggtttc tggagaagtt aaagcctgaa ggtctccacc agctcctggc
      421 cgggttcgag gacaagtcag cctatgcgct ctgcacgttt gcactcagca ccggggaccc
      481 aagccagccc gtgcgcctgt tcaggggccg gacctcgggc cggatcgtgg cacccagagg
      541 ctgccaggac tttggctggg acccctgctt tcagcctgat ggatatgagc agacgtacgc
      601 agagatgcct aaggcggaga agaacgctgt ctcccatcgc ttccgggccc tgctggagct
      661 gcaggagtac tttggcagtt tggcagcttg acttctgcag ctggaggagc atatgctcga
      721 ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      781 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      841 aggaactata tccggatatc ccgcaagagg cccggcagta cc
//
