LOCUS       sequence-100234-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Serves as a shuttle vector for target oligo before insertion into 
            Cas9 destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
  TITLE     CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a 
            new web-tool and a modified Cas9 enzyme.
  JOURNAL   GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
  PUBMED    26479970
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GM Crops 
            Food."; date: "2015"; volume: "6(4):243-52. doi"; pages: " 
            10.1080/21645698.2015.1106063"
COMMENT     Sequence Label: pBlu/gRNA
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..226
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    343..364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(379..384,385..402,403..409)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    417..433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     422..444
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     441..457
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     441..457
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        478..496
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     524..540
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     525..541
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      121 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      181 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
      241 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      301 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
      361 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
      421 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat
      481 taaccctcac taaagggaac aaaagctggt accgggcccc ccctcgaggt cgacggtatc
      541 gataagcttg atatcgaatt caagctttcg ttgaacaacg gaaactcgac ttgccttccg
      601 cacaatacat catttcttct tagctttttt tcttcttctt cgttcataca gttttttttt
      661 gtttatcagc ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat
      721 tcggagtttt tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa
      781 ccttcaagaa tttg
//
