LOCUS       sequence-100236-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to purify human ITPA from bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Porta J, Kolar C, Kozmin SG, Pavlov YI, Borgstahl GE
  TITLE     Structure of the orthorhombic form of human inosine triphosphate 
            pyrophosphatase.
  JOURNAL   Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt 
            11):1076-81. Epub 2006 Oct 25.
  PUBMED    17077483
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr Sect F Struct Biol Cryst Commun."; date: "2006-11-1"; 
            volume: "62(Pt 11)"; pages: "1076-81. Epub 2006 Oct 25"
COMMENT     Sequence Label: pET15b-ITPA
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(646..663)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(673..690)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             709..731
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    746..770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctttcgggct ttgttagcag ccggatcctc gagcatatgc tcctccagct gcagaagtca
       61 agctgccaaa ctgccaaagt actcctgcag ctccagcagg gcccggaagc gatgggagac
      121 agcgttcttc tccgccttag gcatctctgc gtacgtctgc tcatatccat caggctgaaa
      181 gcaggggtcc cagccaaagt cctggcagcc tctgggtgcc acgatccggc ccgaggtccg
      241 gcccctgaac aggcgcacgg gctggcttgg gtccccggtg ctgagtgcaa acgtgcagag
      301 cgcataggct gacttgtcct cgaacccggc caggagctgg tggagacctt caggctttaa
      361 cttctccaga aaccacttta tgtaggggcc ggggagccct ccaagggcat tgaagcacag
      421 acaagtgtcc tcaaccagca cgggcccctg tacctggcga actgcctcct gacatttctg
      481 tatggaaatc tcatccggct ccccctggta ctccggcagg tcaattttct gtgccaccaa
      541 agtgcatgga aacttatctc ctagaatctg aacgacctcc tccagcttct tggcgttccc
      601 cgttacaaac acgatcttct tccccaccaa tgaggccgcc atatggctgc cgcgcggcac
      661 caggccgctg ctgtgatgat gatgatgatg gctgctgccc atggtatatc tccttcttaa
      721 agttaaacaa aattatttct agaggggaat tgttatccgc tcacaattcc cctataatga
      781 gtcgtattaa atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtgg
//
