LOCUS       sequence-100238-        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template vector for generating puromycin resistance cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   MacDonald C, Piper RC
  TITLE     Puromycin and Methotrexate Resistance Cassettes and Optimized 
            cre-recombinase Expression Plasmids for use in Yeast.
  JOURNAL   Yeast. 2015 Feb 16. doi: 10.1002/yea.3069.
  PUBMED    25688547
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Feb 16. doi: 10.1002/yea.3069."
COMMENT     Sequence Label: pPL5617_pUG_PuroR
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        60..78
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     60..77
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    129..162
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        217..560
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat acacatacga
       61 tttaggtgac actatagaac gcggccgcca gctgaagctt cgtacgctgc aggtcgacaa
      121 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtcta gagatctgtt
      181 tagcttgcct cgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc
      241 cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag
      301 cccatacatc cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga
      361 agcaaaaatt acggctcctc gctgcagacc tgcgagcagg gaaacgctcc cctcacagac
      421 gcgttgaatt gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca
      481 ctgaggttct tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc
      541 acatccgaac ataaacaacc atgactgaat ataaaccaac tgttagattg gctactagag
      601 atgatgttcc aagagctgtt agaactttgg ctgctgcttt cgctgattat ccagctacta
      661 gacatactgt tgatccagat agacatattg aaagagttac tgaattgcaa gaattgttct
      721 tgactagagt tggtttggat attggtaaag tttgggttgc tgatgatggt gctgctgttg
      781 ctgtttggac tactccagaa tctgttgaag ctggtgctgt tttcgctgaa attggtccaa
      841 gaatggctga attgtctggt tctagattgg ctgctcaaca acaaatggaa ggtttgttgg
      901 ctccacatag accaaaagaa ccagcttggt tcttggctac tgttggtgtt tctccagatc
      961 atcaaggtaa aggtttgggt tctgctgttg ttttgccagg tgttgaagct gctgaaagag
     1021 ctggtgttcc agctttcttg gaaacttctg ctccaagaaa tttg
//
