LOCUS       sequence-100239-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to purify human ITPA from bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Stepchenkova EI, Tarakhovskaya ER, Spitler K, Frahm C, Menezes MR, 
            Simone PD, Kolar C, Marky LA, Borgstahl GE, Pavlov YI
  TITLE     Functional study of the P32T ITPA variant associated with drug 
            sensitivity in humans.
  JOURNAL   J Mol Biol. 2009 Sep 25;392(3):602-13. doi: 
            10.1016/j.jmb.2009.07.051. Epub 2009 Jul 23.
  PUBMED    19631656
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2009.07.051"; journalName: "J Mol Biol"; date: 
            "2009-09-25- 25"; volume: "392"; issue: "3"; pages: "602-13"
COMMENT     Sequence Label: pET15b-ITPA_P32T
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..103
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(771..789)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca
       61 tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgg cggcctcatt
      121 ggtggggaag aagatcgtgt ttgtaacggg gaacgccaag aagctggagg aggtcgttca
      181 gattctagga gataagttta cctgcacttt ggtggcacag aaaattgacc tgccggagta
      241 ccagggggag ccggatgaga tttccataca gaaatgtcag gaggcagttc gccaggtaca
      301 ggggcccgtg ctggttgagg acacttgtct gtgcttcaat gcccttggag ggctccccgg
      361 cccctacata aagtggtttc tggagaagtt aaagcctgaa ggtctccacc agctcctggc
      421 cgggttcgag gacaagtcag cctatgcgct ctgcacgttt gcactcagca ccggggaccc
      481 aagccagccc gtgcgcctgt tcaggggccg gacctcgggc cggatcgtgg cacccagagg
      541 ctgccaggac tttggctggg acccctgctt tcagcctgat ggatatgagc agacgtacgc
      601 agagatgcct aaggcggaga agaacgctgt ctcccatcgc ttccgggccc tgctggagct
      661 gcaggagtac tttggcagtt tggcagcttg acttctgcag ctggaggagc atatgctcga
      721 ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      781 ataactagca taaccccctt ggggcctcta aacgggtctt gaggggtttt tgctgaaagg
      841 aggaactata tccggatatc ccgcaagagg ccccggcagt accggcataa ccaagcctat
      901 gcctacagca t
//
