LOCUS       sequence-100240-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template vector for generating puromycin resistance cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   MacDonald C, Piper RC
  TITLE     Puromycin and Methotrexate Resistance Cassettes and Optimized 
            cre-recombinase Expression Plasmids for use in Yeast.
  JOURNAL   Yeast. 2015 Feb 16. doi: 10.1002/yea.3069.
  PUBMED    25688547
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Feb 16. doi: 10.1002/yea.3069."
COMMENT     Sequence Label: pPL5617_pUG_PuroR
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(134..167)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(211..408)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag gttaacctgg cttatcgaaa
       61 ttaatacgac tcactatagg gagaccggca gatccgcggc cgcataggcc actagtggat
      121 ctgatatcac ctaataactt cgtatagcat acattatacg aagttatatt aagggttctc
      181 gagatggcgg cgttagtatc gaatcgacag cagtatagcg accagcattc acatacgatt
      241 gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg
      301 aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa
      361 ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac tctttaagca
      421 cctggttttc tagtcataca ccaagtagct ctatcttttg gacattcaac atcagcagta
      481 acagtgaaac ccaatctttc atagaatggc aaatttcttg gagcagaagt ttccaagaaa
      541 gctggaacac cagctctttc agcagcttca acacctggca aaacaacagc agaacccaaa
      601 cctttacctt gatgatctgg agaaacacca acagtagcca agaaccaagc tggttctttt
      661 ggtctatgtg gagccaacaa accttccatt tgttgttgag cagccaatct agaaccagac
      721 aattcagcca ttcttggacc aatttcagcg aaaacagcac cagcttcaac agattctgga
      781 gtagtccaaa cagcaacagc agcaccatca tcagcaaccc aaactttacc aatatccaaa
      841 ccaactctag tcaagaacaa ttcttgcaat tcagtaactc tttcaatatg tctatctgga
      901 tcaacagtat gtctagtagc tggataatca gcgaaagcag cagccaaagt tctaacagct
      961 cttggaacat catctctagt agccaatcta acagttggtt ta
//
