LOCUS       sequence-100241-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to purify human ITPA from bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Stepchenkova EI, Tarakhovskaya ER, Spitler K, Frahm C, Menezes MR, 
            Simone PD, Kolar C, Marky LA, Borgstahl GE, Pavlov YI
  TITLE     Functional study of the P32T ITPA variant associated with drug 
            sensitivity in humans.
  JOURNAL   J Mol Biol. 2009 Sep 25;392(3):602-13. doi: 
            10.1016/j.jmb.2009.07.051. Epub 2009 Jul 23.
  PUBMED    19631656
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2009.07.051"; journalName: "J Mol Biol"; date: 
            "2009-09-25- 25"; volume: "392"; issue: "3"; pages: "602-13"
COMMENT     Sequence Label: pET15b-ITPA_P32T
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(636..653)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(663..680)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             699..721
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    736..760
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(760..779)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(761..779)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttgttagcag ccggatcctc gagcatatgc tcctccagct gcagaagtca agctgccaaa
       61 ctgccaaagt actcctgcag ctccagcagg gcccggaagc gatgggagac agcgttcttc
      121 tccgccttag gcatctctgc gtacgtctgc tcatatccat caggctgaaa gcaggggtcc
      181 cagccaaagt cctggcagcc tctgggtgcc acgatccggc ccgaggtccg gcccctgaac
      241 aggcgcacgg gctggcttgg gtccccggtg ctgagtgcaa acgtgcagag cgcataggct
      301 gacttgtcct cgaacccggc caggagctgg tggagacctt caggctttaa cttctccaga
      361 aaccacttta tgtaggggcc ggggagccct ccaagggcat tgaagcacag acaagtgtcc
      421 tcaaccagca cgggcccctg tacctggcga actgcctcct gacatttctg tatggaaatc
      481 tcatccggct ccccctggta ctccggcagg tcaattttct gtgccaccaa agtgcaggta
      541 aacttatctc ctagaatctg aacgacctcc tccagcttct tggcgttccc cgttacaaac
      601 acgatcttct tccccaccaa tgaggccgcc atatggctgc cgcgcggcac caggccgctg
      661 ctgtgatgat gatgatgatg gctgctgccc atggtatatc tccttcttaa agttaaacaa
      721 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa
      781 ttttcgcggg aatcgagatc tcgatcctct acgccggacg catcgtggcc ggca
//
