LOCUS       sequence-100243-        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template vector for generating methotrexate resistance 
            cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   MacDonald C, Piper RC
  TITLE     Puromycin and Methotrexate Resistance Cassettes and Optimized 
            cre-recombinase Expression Plasmids for use in Yeast.
  JOURNAL   Yeast. 2015 Feb 16. doi: 10.1002/yea.3069.
  PUBMED    25688547
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Feb 16. doi: 10.1002/yea.3069."
COMMENT     Sequence Label: pPL5618_pUG_MtxR
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        59..77
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     59..76
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    128..161
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        216..559
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
       61 ttaggtgaca ctatagaacg cggccgccag ctgaagcttc gtacgctgca ggtcgacaac
      121 ccttaatata acttcgtata atgtatgcta tacgaagtta ttaggtctag agatctgttt
      181 agcttgcctc gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc
      241 ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc
      301 ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa
      361 gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg
      421 cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac
      481 tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca
      541 catccgaaca taaacaacca tggttggttc tttgaactgt atcgttgctg tttctcaaaa
      601 catgggtatc ggtaagaacg gtgacttccc atggccacca ttgagaaacg aatctagata
      661 cttccaaaga atgaccacca cctcttctgt tgaaggtaag caaaacttgg ttatcatggg
      721 taagaagacc tggttctcta tcccagaaaa gaacagacca ttgaagggta gaatcaactt
      781 ggttttgtct agagaattga aggaaccacc acaaggtgct cacttcttgt ctagatcttt
      841 ggacgacgct ttgaagttga ccgaacaacc agaattggct aacaaggttg acatggtttg
      901 gatcgttggt ggttcttctg tttacaagga agctatgaac cacccaggtc acttgaagtt
      961 gttcgttacc agaatcatgc aagacttcga atctgacacc ttcttcccag aaatcgactt
     1021 ggaaaagtac aagttgttgc cagaataccc aggtgttttg tctgacgttc aagaagaaaa
     1081 gggtatc
//
