LOCUS       sequence-100245-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template vector for generating methotrexate resistance 
            cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   MacDonald C, Piper RC
  TITLE     Puromycin and Methotrexate Resistance Cassettes and Optimized 
            cre-recombinase Expression Plasmids for use in Yeast.
  JOURNAL   Yeast. 2015 Feb 16. doi: 10.1002/yea.3069.
  PUBMED    25688547
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Feb 16. doi: 10.1002/yea.3069."
COMMENT     Sequence Label: pPL5618_pUG_MtxR
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(134..167)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(227..424)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag gttaacctgg cttatcgaaa
       61 ttaatacgac tcactatagg gagaccggca gatccgcggc cgcataggcc actagtggat
      121 ctgatatcac ctaataactt cgtatagcat acattatacg aagttatatt aagggttctc
      181 gagagctcgt ttaaactgga tggcggcgtt agtatcgaat cgacagcagt atagcgacca
      241 gcattcacat acgattgacg catgatatta ctttctgcgc acttaacttc gcatctgggc
      301 agatgatgtc gaggcgaaaa aaaatataaa tcacgctaac atttgattaa aatagaacaa
      361 ctacaatata aaaaaactat acaaatgaca agttcttgaa aacaagaatc tttttattgt
      421 cagtactctt tagtcgttct tttcgtaaac ttcgagcttg tacttgatac ccttttcttc
      481 ttgaacgtca gacaaaacac ctgggtattc tggcaacaac ttgtactttt ccaagtcgat
      541 ttctgggaag aaggtgtcag attcgaagtc ttgcatgatt ctggtaacga acaacttcaa
      601 gtgacctggg tggttcatag cttccttgta aacagaagaa ccaccaacga tccaaaccat
      661 gtcaaccttg ttagccaatt ctggttgttc ggtcaacttc aaagcgtcgt ccaaagatct
      721 agacaagaag tgagcacctt gtggtggttc cttcaattct ctagacaaaa ccaagttgat
      781 tctacccttc aatggtctgt tcttttctgg gatagagaac caggtcttct tacccatgat
      841 aaccaagttt tgcttacctt caacagaaga ggtggtggtc attctttgga agtatctaga
      901 ttcgtttctc aatggtggcc atgggaagtc accgttctta ccgataccca tgttttgaga
      961 aacagcaacg atacagttca aagaaccaac catggttgtt tatgttcgga tgtgatgtga
     1021 gaactgtatc ctagcaagat tttaaaagga agtatatgaa agaagaacct cagtggcaaa
     1081 tcctaacc
//
