LOCUS       sequence-100257-        1363 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human FK506 binding protein 1A (FKBP1A) tagged with 
            mCherry, Tom20, and AIP with TD mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: Tom20-mChF-AIP(TD)
FEATURES             Location/Qualifiers
     source          1..1363
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             149..856
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(294..312)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     562..581
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             869..1189
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 cgctagcgct accggtgaat tcgccaccat ggtgggtcgg aacagcgcca tcgccgcggg
       61 cgtgtgcggt gccctcttca tagggtactg catctacttt gaccgcaaaa gacgaagtga
      121 ccccaacttc ggatccggag caagtggaat ggtgagcaag ggcgaggagg ataacatggc
      181 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      241 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      301 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
      361 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
      421 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      481 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
      541 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
      601 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
      661 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
      721 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
      781 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
      841 ggacgaactg tacaagagtg ctggtggtgg agtgcaggtg gaaaccatct ccccaggaga
      901 cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg cactacaccg ggatgcttga
      961 agatggaaag aaatttgatt cctcccggga cagaaacaag ccctttaagt ttatgctagg
     1021 caagcaggag gtgatccgag gctgggaaga aggggttgcc cagatgagtg tgggtcagag
     1081 agccaaactg actatatctc cagattatgc ctatggtgcc actgggcacc caggcatcat
     1141 cccaccacat gccactctcg tcttcgatgt ggagcttcta aaactggaaa gtgctggtgg
     1201 tagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggtc ctcgagctca
     1261 agcttcgaat tcaggatcag gagagggatc aaccaagatg agaagagtgg ctgatctggt
     1321 ggaccttgga accggaggat cagagctctg actgcaggga tcc
//
