LOCUS       sequence-100261-         474 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), 
            expresses RFP and hygromycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Li HL, Fujimoto N, Sasakawa N, Shirai S, Ohkame T, Sakuma T, Tanaka 
            M, Amano N, Watanabe A, Sakurai H, Yamamoto T, Yamanaka S, Hotta A
  TITLE     Precise Correction of the Dystrophin Gene in Duchenne Muscular 
            Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and 
            CRISPR-Cas9.
  JOURNAL   Stem Cell Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 
            10.1016/j.stemcr.2014.10.013.
  PUBMED    25434822
REFERENCE   2  (bases 1 to 474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 
            10.1016/j.stemcr.2014.10.013."
COMMENT     Sequence Label: pHL-H1-ccdB-mEF1a-RiH
FEATURES             Location/Qualifiers
     source          1..474
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(195..213)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aacccttttt taggtgttgt gaaaaccacc gctaattcaa agcaaccggt gatatcggat
       61 caccatggcc tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga
      121 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
      181 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
      241 catcctgtcc cctcagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat
      301 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
      361 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
      421 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgca
//
