LOCUS       sequence-100263-        1643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human golbin B1 and FK506 binding protein 1A (FKBP1A) 
            tagged with mCherry and AIP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: AIP(WT)-mChF-giantin
FEATURES             Location/Qualifiers
     source          1..1643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      129..138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             135..842
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(280..298)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     548..567
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             855..1175
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 cagagctggt ttagtgancc gtcagatccg ctagcaccgc catgggatca ggagagggat
       61 caaccaagat gagaagagtg gctactctgg tggaccttgg aaccggagga tcagagctca
      121 taccggtcgc caccatggta agcaagggcg aggaggataa catggccatc atcaaggagt
      181 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      241 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      301 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
      361 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      421 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
      481 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
      541 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
      601 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
      661 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
      721 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
      781 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
      841 agagtgctgg tggtggagtg caggtggaaa ccatctcccc aggagacggg cgcaccttcc
      901 ccaagcgcgg ccagacctgc gtggtgcact acaccgggat gcttgaagat ggaaagaaat
      961 ttgattcctc ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga
     1021 tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc aaactgacta
     1081 tatctccaga ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca
     1141 ctctcgtctt cgatgtggag cttctaaaac tggaaagtgc tggtggtagt gctggtggta
     1201 gtgctggtgg tagtgctggt ggtagtgctg gtggtcctcg aggagaaccg cagcaaagct
     1261 tttctgaagc tcagcagcag ctatgcaaca ccagacagga agtgaatgaa ttaaggaagc
     1321 tgctggaaga agaacgagac caaagagtgg ctgctgagaa tgctctctct gtggccgagg
     1381 agcagatcag acggttagag cacagtgaat gggactcttc ccggactcct atcattggct
     1441 cctgtggcac tcaggagcag gcactgttaa tagatcttac aagcaacagt tgtcgaagga
     1501 cccggagtgg cgttggatgg aagcgagtcc tgcgttcact ctgtcattca cggacccgag
     1561 tgccacttct agcagccatc tactttctaa tgattcatgt cctgctcatt ctgtgnttta
     1621 cgggccatct atagggatcc acc
//
