LOCUS       sequence-100265-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knock-in donor vector to insert Exon 44 in front of Exon 45 of human
            DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette 
            flanked by loxP sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Li HL, Fujimoto N, Sasakawa N, Shirai S, Ohkame T, Sakuma T, Tanaka 
            M, Amano N, Watanabe A, Sakurai H, Yamamoto T, Yamanaka S, Hotta A
  TITLE     Precise Correction of the Dystrophin Gene in Duchenne Muscular 
            Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and 
            CRISPR-Cas9.
  JOURNAL   Stem Cell Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 
            10.1016/j.stemcr.2014.10.013.
  PUBMED    25434822
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 
            10.1016/j.stemcr.2014.10.013."
COMMENT     Sequence Label: pENTR-DMD-Donor
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctggcgcc ggaaccaatt cagtcgactg
      121 gatccaagag cttggcaaaa gaacgaagtt ttcattgttc ataacaatag tctcatttgg
      181 taaataaagg ccaagtcttc ctttacgaaa caagacacat taacatcaac aactggaagc
      241 ataatacaaa atcccattta taaactctct aggctttcca actgcagcag cacgcatttg
      301 gctttctgtg ccttcaatac attccaaggg aaatttaaat gatgattgaa tttgacagta
      361 acctttttga ggttttgttt tccccattaa acttgtacct ctttggctca agttcccctt
      421 caagaatgta ttcacaaatg tggtgaaact agaggtaagt gacactatca ctttttttag
      481 cttcatagtc atattcatag ctatttttaa aactaagcaa agatctgtct ttcctacaaa
      541 acaatcattt ataattgctt tctaaaatct tcttgaaaaa caactgagat tcagcttgtt
      601 gaagttaaaa tatattgaag atattcacct ttaagcaatc atgggtgatt tttaaagcaa
      661 acttcaagtt taaaatagca gaaaaccact aactagccac aagtatatat tttagtatat
      721 gaaa
//
