LOCUS       sequence-100266-        2522 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Homo sapiens lysosomal-associated membrane protein 1 
            (LAMP1) tagged with mCherry and AIP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: LAMP-mChF-AIP
FEATURES             Location/Qualifiers
     source          1..2522
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(33..116,117..1283)
                     /codon_start=1
                     /product="human lysosome-associated membrane glycoprotein 
                     1"
                     /label=LAMP1
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMAN
                     FSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNF
                     TRNATRYSVQLMSFAYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHM
                     NNVTVTPHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKY
                     NVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSE
                     GTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNA
                     EEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIV
                     LIAYLVGRKRSHAGYQTI"
     regulatory      1293..1302
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1299..2006
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1444..1462)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1712..1731
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2019..2339
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 cnnncnctag cgctaccggt ganttcgcca ccatggcggc ccccggcagc gcccggcgac
       61 ccctgctgct gctactgctg ttgctgctgc tcggcctcat gcattgtgcg tcagcagcaa
      121 tgtttatggt gaaaaatggc aacgggaccg cgtgcataat ggccaacttc tctgctgcct
      181 tctcagtgaa ctacgacacc aagagtggcc ctaagaacat gacctttgac ctgccatcag
      241 atgccacagt ggtgctcaac cgcagctcct gtggaaaaga gaacacttct gaccccagtc
      301 tcgtgattgc ttttggaaga ggacatacac tcactctcaa tttcacgaga aatgcaacac
      361 gttacagcgt ccagctcatg agttttgctt ataacttgtc agacacacac cttttcccca
      421 atgcgagctc caaagaaatc aagactgtgg aatctataac tgacatcagg gcagatatag
      481 ataaaaaata cagatgtgtt agtggcaccc aggtccacat gaacaacgtg accgtaacgc
      541 cccatgatgc caccatccag gcgtaccttt ccaacagcag cttcagcagg ggagagacac
      601 gctgtgaaca agacaggcct tccccaacca cagcgccccc tgcgccaccc agcccctcgc
      661 cctcacccgt gcccaagagc ccctctgtgg acaagtacaa cgtgagcggc accaacggga
      721 cctgcctgct ggccagcatg gggctgcagc tgaacctcac ctatgagagg aaggacaaca
      781 cgacggtgac aaggcttctc aacatcaacc ccaacaagac ctcggccagc gggagctgcg
      841 gcgcccacct ggtgactctg gagctgcaca gcgagggcac caccgtcctg ctcttccagt
      901 tcgggatgaa tgcaagttct agccggtttt tcctacaagg aatccagttg aatacaattc
      961 ttcctgacgc cagagaccct gcctttaaag ctgccaacgg ctccctgcga gcgctgcagg
     1021 ccacagtcgg caattcctac aagtgcaacg cggaggagca cgtccgtgtc acgaaggcgt
     1081 tttcagtcaa tatattcaaa gtgtgggtcc aggctttcaa ggtggaaggt ggccagtttg
     1141 gctctgtgga ggagtgtctg ctggacgaga acagcatgct gatccccatc gctgtgggtg
     1201 gtgccctggc ggggctggtc ctcatcgtcc tcatcgccta cctcgtcggc aggaagagga
     1261 gtcacgcagg ctaccagact atcccaccgg tcgccaccat ggtaagcaag ggcgaggagg
     1321 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     1381 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     1441 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     1501 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     1561 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     1621 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     1681 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag actatgggct
     1741 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     1801 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     1861 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     1921 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     1981 ccggcggcat ggacgaactg tacaagagtg ctggtggtgg agtgcaggtg gaaaccatct
     2041 ccccaggaga cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg cactacaccg
     2101 ggatgcttga agatggaaag aaatttgatt cctcccggga cagaaacaag ccctttaagt
     2161 ttatgctagg caagcaggag gtgatccgag gctgggaaga aggggttgcc cagatgagtg
     2221 tgggtcagag agccaaactg actatatctc cagattatgc ctatggtgcc actgggcacc
     2281 caggcatcat cccaccacat gccactctcg tcttcgatgt ggagcttcta aaactggaaa
     2341 gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggtc
     2401 ctcgagctca agcttcgaat tcaggatcag gagagggatc aaccaagatg agaagagtgg
     2461 ctactctggt ggaccttgga accggaggat cagagctctg actgcagggn nccaccnnnc
     2521 na
//
