LOCUS       sequence-100271-        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human FK506 binding protein 1A (FKBP1A) tagged with 
            mCherry and AIP (with TD mutation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: mChF-AIP (TD)
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(182..502)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     primer_bind     complement(790..809)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gnnnggtggn nccctgcagt cagagctctg atcctccggt tccaaggtcc accagatcag
       61 ccactcttct catcttggtt gatccctctc ctgatcctga attcgaagct tgagctcgag
      121 gaccaccagc actaccacca gcactaccac cagcactacc accagcacta ccaccagcac
      181 tttccagttt tagaagctcc acatcgaaga cgagagtggc atgtggtggg atgatgcctg
      241 ggtgcccagt ggcaccatag gcataatctg gagatatagt cagtttggct ctctgaccca
      301 cactcatctg ggcaacccct tcttcccagc ctcggatcac ctcctgcttg cctagcataa
      361 acttaaaggg cttgtttctg tcccgggagg aatcaaattt ctttccatct tcaagcatcc
      421 cggtgtagtg caccacgcag gtctggccgc gcttggggaa ggtgcgcccg tctcctgggg
      481 agatggtttc cacctgcact ccaccaccag cactcttgta cagctcgtcc atgccgccgg
      541 tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg
      601 aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg
      661 ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct
      721 gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc
      781 agcccatggt cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct
      841 tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc
      901 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca
      961 agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgan
     1021 gggacaggat gtcccag
//
