LOCUS       sequence-100272-        1225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human FK506 binding protein 1A (FKBP1A) tagged with 
            mCherry and AIP (with TE mutation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: mChF-AIP (TE)
FEATURES             Location/Qualifiers
     source          1..1225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..723
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(161..179)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     429..448
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             736..1056
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 ccaccggtcg ccaccatggt aagcaagggc gaggaggata acatggccat catcaaggag
       61 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
      121 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      181 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
      241 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
      301 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
      361 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
      421 tccgacggcc ccgtaatgca gaagaagact atgggctggg aggcctcctc cgagcggatg
      481 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
      541 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      601 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      661 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      721 aagagtgctg gtggtggagt gcaggtggaa accatctccc caggagacgg gcgcaccttc
      781 cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa
      841 tttgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg
      901 atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc caaactgact
      961 atatctccag attatgccta tggtgccact gggcacccag gcatcatccc accacatgcc
     1021 actctcgtct tcgatgtgga gcttctaaaa ctggaaagtg ctggtggtag tgctggtggt
     1081 agtgctggtg gtagtgctgg tggtagtgct ggtggtcctc gagctcaagc ttcgaattca
     1141 ggatcaggag agggatcaac caagatgaga agagtggctg aactggtgga ccttggaacc
     1201 ggaggatcag agctctgact gcagg
//
