LOCUS       sequence-100283-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to express human Pol iota recoded to yeast codons in yeast .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Makarova AV, Grabow C, Gening LV, Tarantul VZ, Tahirov TH, Bessho T,
            Pavlov YI
  TITLE     Inaccurate DNA synthesis in cell extracts of yeast producing active 
            human DNA polymerase iota.
  JOURNAL   PLoS One. 2011 Jan 31;6(1):e16612. doi: 
            10.1371/journal.pone.0016612.
  PUBMED    21304950
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-01-31"; volume: "6(1):e16612. doi"; pages: " 
            10.1371/journal.pone.0016612"
COMMENT     Sequence Label: pESC-URA-hPOLISc
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(45..234)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(573..584)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggatcttcga gcgtcccaaa
       61 accttctcaa gcaaggtttt cagtataatg ttacatgcgt acacgcgtct gtacagaaaa
      121 aaaagaaaaa tttgaaatat aaataacgtt cttaatacta acataactat aaaaaaataa
      181 atagggacct agacttcagg ttgtctaact ccttcctttt cggttagagc ggatcttagc
      241 tagcttactt gtgaccgatg tggaagtcag aaccggttct cttccattca gccaacaatt
      301 ccttttgaac agcttctggc aattcgtaga aaacttgtgg gtcgatgtca gatgggaagg
      361 tgatcttttc gtcaacagag tctggttctc tgttttcggt caaaccttcg tgagagtcgg
      421 tagcaacggt ttgcttgtga gagtcggtgg tgtggtttct agagaacaat tgttcagatt
      481 gcaagtttgg gaaagagtgg aaagcagaaa cagctgggtt agagttggtg aagtggaaac
      541 cttgtggttc ctttggacct tgagagattc tttcgtcctt caatctgttg tccaagtagt
      601 aagagtagtc cttttgagaa gacatgtaag aagaagaaga agagttgaaa ccagaggtac
      661 ctggttcaca tggagaaaca gaagaaactt gcttagaaga agacaagtgg tctcttgggt
      721 tgattgggat gtcttgcatt tgcttcttag agaa
//
