LOCUS       sequence-100292-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a TALEN protein specific for mouse ROSA26 locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Kasparek P, Krausova M, Haneckova R, Kriz V, Zbodakova O, Korinek V,
            Sedlacek R
  TITLE     Efficient gene targeting of the Rosa26 locus in mouse zygotes using 
            TALE nucleases.
  JOURNAL   FEBS Lett. 2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014.
  PUBMED    25241166
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014."
COMMENT     Sequence Label: TALEN-mROSA26 KKR
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      60..69
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             69..134
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             141..161
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcccagttc aattacagct cttaaggcta gagtacttaa tacgactcac tataggctag
       61 ccaccatgga ctataaggac cacgacggag actacaagga tcatgatatt gattacaaag
      121 acgatgacga taagatggcc ccaaagaaga agcggaaggt cggtatccac ggagtcccag
      181 cagccgtaga tttgagaact ttgggatatt cacagcagca gcaggaaaag atcaagccca
      241 aagtgaggtc gacagtcgcg cagcatcacg aagcgctggt gggtcatggg tttacacatg
      301 cccacatcgt agccttgtcg cagcaccctg cagcccttgg cacggtcgcc gtcaagtacc
      361 aggacatgat tgcggcgttg ccggaagcca cacatgaggc gatcgtcggt gtggggaaac
      421 agtggagcgg agcccgagcg cttgaggccc tgttgacggt cgcgggagag ctgagagggc
      481 ctccccttca gctggacacg ggccagttgc tgaagatcgc gaagcgggga ggagtcacgg
      541 cggtcgaggc ggtgcacgcg tggcgcaatg cactgacggg tgcccccctg aacctgaccc
      601 cggaccaagt ggtggctatc gccagccacg atggcggcaa gcaagcgctc gaaacggtgc
      661 agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta
      721 tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
      781 tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc aacggtggcg
      841 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
      901 tgaccccgga ccaagtggtg gctatcgcca gcaacaatgg cggca
//
