LOCUS       sequence-100296-        3302 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a TALEN protein specific for mouse ROSA26 locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Kasparek P, Krausova M, Haneckova R, Kriz V, Zbodakova O, Korinek V,
            Sedlacek R
  TITLE     Efficient gene targeting of the Rosa26 locus in mouse zygotes using 
            TALE nucleases.
  JOURNAL   FEBS Lett. 2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014.
  PUBMED    25241166
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014."
COMMENT     Sequence Label: TALEN-mROSA26 KKR
FEATURES             Location/Qualifiers
     source          1..3302
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      60..69
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             69..134
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             141..161
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcccagttc aattacagct cttaaggcta gagtacttaa tacgactcac tataggctag
       61 ccaccatgga ctataaggac cacgacggag actacaagga tcatgatatt gattacaaag
      121 acgatgacga taagatggcc ccaaagaaga agcggaaggt cggtatccac ggagtcccag
      181 cagccgtaga tttgagaact ttgggatatt cacagcagca gcaggaaaag atcaagccca
      241 aagtgaggtc gacagtcgcg cagcatcacg aagcgctggt gggtcatggg tttacacatg
      301 cccacatcgt agccttgtcg cagcaccctg cagcccttgg cacggtcgcc gtcaagtacc
      361 aggacatgat tgcggcgttg ccggaagcca cacatgaggc gatcgtcggt gtggggaaac
      421 agtggagcgg agcccgagcg cttgaggccc tgttgacggt cgcgggagag ctgagagggc
      481 ctccccttca gctggacacg ggccagttgc tgaagatcgc gaagcgggga ggagtcacgg
      541 cggtcgaggc ggtgcacgcg tggcgcaatg cactgacggg tgcccccctg aacctgaccc
      601 cggaccaagt ggtggctatc gccagccacg atggcggcaa gcaagcgctc gaaacggtgc
      661 agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta
      721 tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
      781 tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc aacggtggcg
      841 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
      901 tgaccccgga ccaagtggtg gctatcgcca gcaacaatgg cggcaagcaa gcgctcgaaa
      961 cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg
     1021 tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc
     1081 cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaacg
     1141 gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc
     1201 atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc aagcaagcgc
     1261 tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc
     1321 aagtggtggc tatcgccagc aacggtggcg gcaagcaagc gctcgaaacg gtgcagcggc
     1381 tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca
     1441 gcaacaatgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc
     1501 aggaccatgg cctgactccg gaccaagtgg tggctatcgc cagccacgat ggcggcaagc
     1561 aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc
     1621 cggaccaagt ggtggctatc gccagcaaca ttggcggcaa gcaagcgctc gaaacggtgc
     1681 agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta
     1741 tcgccagcaa cattggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
     1801 tgtgccagga ccatggcctg actccggacc aagtggtggc tatcgccagc cacgatggcg
     1861 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
     1921 tgaccccgga ccaagtggtg gctatcgcca gcaacggtgg cggcaagcaa gcgctcgaaa
     1981 cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgactccg gaccaagtgg
     2041 tggctatcgc cagccacgat ggcggcaagc aagcgctcga aacggtgcag cggctgttgc
     2101 cggtgctgtg ccaggaccat ggcctgactc cggaccaagt ggtggctatc gccagccacg
     2161 atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc
     2221 atggcctgac cccggaccaa gtggtggcta tcgccagcaa cattggcggc aagcaagcgc
     2281 tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc
     2341 aagtggtggc tatcgccagc aacaatggcg gcaagcaagc gctcgaaacg gtgcagcggc
     2401 tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca
     2461 gcaacggtgg cggcaagcaa gcgctcgaaa gcattgtggc ccagctgagc cggcctgatc
     2521 cggcgttggc cgcgttgacc aacgaccacc tcgtagcgct ggcctgcctc ggcggacgac
     2581 ccgccttgga tgcggtgaag aaggggctcc cgcacgcgcc tgcattgatt aagcggacca
     2641 acagaaggat tcccgagagg acatcacatc gagtggcagg ttcccaactc gtgaagagtg
     2701 aacttgagga gaaaaagtcg gagctgcggc acaaattgaa atacgtaccg catgaataca
     2761 tcgaacttat cgaaattgct aggaactcga ctcaagacag aatccttgag atgaaggtaa
     2821 tggagttctt tatgaaggtt tatggatacc gagggaagca tctcggtgga tcacgaaaac
     2881 ccgacggagc aatctatacg gtggggagcc cgattgatta cggagtgatc gtcgacacga
     2941 aagcctacag cggtgggtac aatcttccca tcgggcaggc agatgagatg caacgttatg
     3001 tcaaagaaaa tcagaccagg aacaaacaca tcaatccaaa tgagtggtgg aaagtgtatc
     3061 cttcatcagt gaccgagttt aagtttttgt ttgtctctgg gcatttcaaa ggcaactata
     3121 aggcccagct cacacggttg aatcgcaaaa cgaactgcaa tggtgcggtt ttgtccgtag
     3181 aggaactgct cattggtgga gaaatgatca aagcgggaac tctgacactg gaagaagtca
     3241 gacgcaagtt taacaatggc gagatcaatt tccgctcata atctagagcg gccgcttcga
     3301 gc
//
