LOCUS       sequence-10030-e        9869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Charpentier X, Gabay JE, Reyes M, Zhu JW, Weiss A, Shuman HA
  TITLE     Chemical genetics reveals bacterial and host cell functions critical
            for type IV effector translocation by Legionella pneumophila.
  JOURNAL   PLoS Pathog. 2009 Jul . 5(7):e1000501.
  PUBMED    19578436
REFERENCE   2  (bases 1 to 9869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Jul . 5(7):e1000501."
COMMENT     Sequence Label: pXDC61
FEATURES             Location/Qualifiers
     source          1..9869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..22,23..29)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    37..53
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     42..64
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             101..886
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     primer_bind     complement(247..266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(953..970)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1123..1209
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1301..1328
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1347..1438
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1889..1991
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1992..2651
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2058..2077)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2983..3377
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            3719..3806
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             5045..6016
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDAPTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGHDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6527..7366
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             7353..8204
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRKRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     9524..9543
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9563..9640)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca
       61 caggaaacag aattcagaag gagatataca tatggctcac ccagaaacgc tggtgaaagt
      121 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      181 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      241 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      301 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      361 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      421 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      481 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      541 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
      601 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      661 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      721 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      781 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
      841 aaatagacag atcgctgaga taggtgcctc actgattaag cattggggaa gcggtacccg
      901 gggatcctct agataagctt ggctgttttg gcggatgaga gaagattttc agcctgatac
      961 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
     1021 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
     1081 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     1141 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     1201 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     1261 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
     1321 ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta
     1381 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1441 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1501 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1561 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     1621 agaacgtgat gtccggcggt gcttttgccg ttacgcacca ccccgtcagt agctgaacag
     1681 gagggacagc tgatagaaac agaagccact ggagcaagca cctcaaaaac accatcatac
     1741 actaaatcag taagttggca gcatcacccg acgcactttg cgccgaataa atacctgtga
     1801 cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct
     1861 gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc
     1921 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
     1981 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     2041 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     2101 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     2161 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaatta cgtatggcaa
     2221 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     2281 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     2341 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     2401 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     2461 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     2521 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     2581 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     2641 gcggggcgta atttttttaa ggcagttatt gtcttcaaat tcccgttgca catagcccgg
     2701 caattccttt ccctgctctg ccataagcgc agcgaatgcc gggtaatact cgtcaacgat
     2761 ctgatagaga agggtttgct cgggtcggtg gctctggtaa cgaccagtat cccgatcccg
     2821 gctggccgtc ctggccgcca catgaggcat gttccgcgtc cttgcaatac tgtgtttaca
     2881 tacagtctat cgcttagcgg aaagttcttt taccctcagc cgaaatgcct gccgttgcta
     2941 gacattgcca gccagtgccc gtcactcccg tactaactgt cacgaacccc tgcaataact
     3001 gtcacgcccc cctgcaataa ctgtcacgaa cccctgcaat aactgtcacg cccccaaacc
     3061 tgcaaaccca gcaggggcgg gggctggcgg ggtgttggaa aaatccatcc atgattatct
     3121 aagaataatc cactaggcgc ggttatcagc gcccttgtgg ggcgctgctg cccttgccca
     3181 atatgcccgg ccagaggccg gatagctggt ctattcgctg cgctaggcta cacaccgccc
     3241 caccgctgcg cggcaggggg aaaggcgggc aaagcccgct aaaccccaca ccaaaccccg
     3301 cagaaatacg ctggagcgct tttagccgct ttagcggcct ttccccctac ccgaagggtg
     3361 ggggcgcgtg tgcagccccg cagggcctgt ctcggtcgat cattcagccc ggctcatcct
     3421 tctggcgtgg cggcagaccg aacaaggcgc ggtcgtggtc gcgttcaagg tacgcatcca
     3481 ttgccgccat gagccgatcc tccggccact cgctgctgtt caccttggcc aaaatcatgg
     3541 cccccaccag caccttgcgc cttgtttcgt tcttgcgctc ttgctgctgt tcccttgccc
     3601 gctcccgctg aatttcggca ttgattcgcg ctcgttgttc ttcgagcttg gccagccgat
     3661 ccgccgcctt gttgctcccc ttaaccatct tgacacccca ttgttaatgt gctgtctcgt
     3721 aggctatcat ggaggcacag cggcggcaat cccgacccta ctttgtaggg gagggcgcac
     3781 ttaccggttt ctcttcgaga aactggccta acggccaccc ttcgggcggt gcgctctccg
     3841 agggccattg catggagccg aaaagcaaaa gcaacagcga ggcagcatgg cgatttatca
     3901 ccttacggcg aaaaccggca gcaggtcggg cggccaatcg gccagggcca aggccgacta
     3961 catccagcgc gaaggcaagt atgcccgcga catggatgaa gtcttgcacg ccgaatccgg
     4021 gcacatgccg gagttcgtcg agcggcccgc cgactactgg gatgctgccg acctgtatga
     4081 acgcgccaat gggcggctgt tcaaggaggt cgaatttgcc ctgccggtcg agctgaccct
     4141 cgaccagcag aaggcgctgg cgtccgagtt cgcccagcac ctgaccggtg ccgagcgcct
     4201 gccgtatacg ctggccatcc atgccggtgg cggcgagaac ccgcactgcc acctgatgat
     4261 ctccgagcgg atcaatgacg gcatcgagcg gcccgccgct cagtggttca agcggtacaa
     4321 cggcaagacc ccggagaagg gcggggcaca gaagaccgaa gcgctcaagc ccaaggcatg
     4381 gcttgagcag acccgcgagg catgggccga ccatgccaac cgggcattag agcgggctgg
     4441 ccacgacgcc cgcattgacc acagaacact tgaggcgcag ggcatcgagc gcctgcccgg
     4501 tgttcacctg gggccgaacg tggtggagat ggaaggccgg ggcatccgca ccgaccgggc
     4561 agacgtggcc ctgaacatcg acaccgccaa cgcccagatc atcgacttac aggaataccg
     4621 ggaggcaata gaccatgaac gcaatcgaca gagtgaagaa atccagaggc atcaacgagt
     4681 tagcggagca gatcgaaccg ctggcccaga gcatggcgac actggccgac gaagcccggc
     4741 aggtcatgag ccagaccaag caggccagcg aggcgcaggc ggcggagtgg ctgaaagccc
     4801 agcgccagac aggggcggca tgggtggagc tggccaaaga gttgcgggag gtagccgccg
     4861 aggtgagcag cgccgcgcag agcgcccgga gcgcgtcgcg ggggtggcac tggaagctat
     4921 ggctaaccgt gatgctggct tccatgatgc ctacggtggt gctgctgatc gcatcgttgc
     4981 tcttgctcga cctgacgcca ctgacaaccg aggacggctc gatctggctg cgcttggtgg
     5041 cccgatgaag aacgacagga ctttgcaggc cataggccga cagctcaagg ccatgggctg
     5101 tgagcgcttc gatatcggcg tcagggacgc acccaccggc cagatgatga accgggaatg
     5161 gtcagccgcc gaagtgctcc agaacacgcc atggctcaag cggatgaatg cccagggcaa
     5221 tgacgtgtat atcaggcccg ccgagcagga gcggcatggt ctggtgctgg tggacgacct
     5281 cagcgagttt gacctggatg acatgaaagc cgagggccgg gagcctgccc tggtagtgga
     5341 aaccagcccg aagaactatc aggcatgggt caaggtggcc gacgccgcag gcggtgaact
     5401 tcgggggcag attgcccgga cgctggccag cgagtacgac gccgacccgg ccagcgccga
     5461 cagccgccac tatggccgct tggcgggctt caccaaccgc aaggacaagc acaccacccg
     5521 cgccggttat cagccgtggg tgctgctgcg tgaatccaag ggcaagaccg ccaccgctgg
     5581 cccggcgctg gtgcagcagg ctggccagca gatcgagcag gcccagcggc agcaggagaa
     5641 ggcccgcagg ctggccagcc tcgaactgcc cgagcggcag cttagccgcc accggcgcac
     5701 ggcgctggac gagtaccgca gcgagatggc cgggctggtc aagcgcttcg gtcatgacct
     5761 cagcaagtgc gactttatcg ccgcgcagaa gctggccagc cggggccgca gtgccgagga
     5821 aatcggcaag gccatggccg aggccagccc agcgctggca gagcgcaagc ccggccacga
     5881 agcggattac atcgagcgca ccgtcagcaa ggtcatgggt ctgcccagcg tccagcttgc
     5941 gcgggccgag ctggcacggg caccggcacc ccgccagcga ggcatggaca ggggcgggcc
     6001 agatttcagc atgtagtgct tgcgttggta ctcacgcctg ttatactatg agtactcacg
     6061 cacagaaggg ggttttatgg aatacgaaaa aagcgcttca gggtcggtct acctgatcaa
     6121 aagtgacaag ggctattggt tgcccggtgg ctttggttat acgtcaaaca aggccgaggc
     6181 tggccgcttt tcagtcgctg atatggccag ccttaacctt gacggctgca ccttgtcctt
     6241 gttccgcgaa gacaagcctt tcggccccgg caagtttctc ggtgactgat atgaaagacc
     6301 aaaaggacaa gcagaccggc gacctgctgg ccagccctga cgctgtacgc caagcgcgat
     6361 atgccgagcg catgaaggcc aaagggatgc gtcagcgcaa gttctggctg accgacgacg
     6421 aatacgaggc gctgcgcgag tgcctggaag aactcagagc ggcgcagggc gggggtagtg
     6481 accccgccag cgcctaacca ccaactgcct gcaaaggagg caatcaatgg ctacccataa
     6541 gcctatcaat attctggagg cgttcgcagc agcgccgcca ccgctggact acgttttgcc
     6601 caacatggtg gccggtacgg tcggggcgct ggtgtcgccc ggtggtgccg gtaaatccat
     6661 gctggccctg caactggccg cacagattgc aggcgggccg gatctgctgg aggtgggcga
     6721 actgcccacc ggcccggtga tctacctgcc cgccgaagac ccgcccaccg ccattcatca
     6781 ccgcctgcac gcccttgggg cgcacctcag cgccgaggaa cggcaagccg tggctgacgg
     6841 cctgctgatc cagccgctga tcggcagcct gcccaacatc atggccccgg agtggttcga
     6901 cggcctcaag cgcgccgccg agggccgccg cctgatggtg ctggacacgc tgcgccggtt
     6961 ccacatcgag gaagaaaacg ccagcggccc catggcccag gtcatcggtc gcatggaggc
     7021 catcgccgcc gataccgggt gctctatcgt gttcctgcac catgccagca agggcgcggc
     7081 catgatgggc gcaggcgacc agcagcaggc cagccggggc agctcggtac tggtcgataa
     7141 catccgctgg cagtcctacc tgtcgagcat gaccagcgcc gaggccgagg aatggggtgt
     7201 ggacgacgac cagcgccggt tcttcgtccg cttcggtgtg agcaaggcca actatggcgc
     7261 accgttcgct gatcggtggt tcaggcggca tgacggcggg gtgctcaagc ccgccgtgct
     7321 ggagaggcag cgcaagagca agggggtgcc ccgtggtgaa gcctaagaac aagcacagcc
     7381 tcagccacgt ccggcacgac ccggcgcact gtctggcccc cggcctgttc cgtgccctca
     7441 agcggggcga gcgcaagcgc agcaagctgg acgtgacgta tgactacggc gacggcaagc
     7501 ggatcgagtt cagcggcccg gagccgctgg gcgctgatga tctgcgcatc ctgcaagggc
     7561 tggtggccat ggctgggcct aatggcctag tgcttggccc ggaacccaag accgaaggcg
     7621 gacggcagct ccggctgttc ctggaaccca agtgggaggc cgtcaccgct gaatgccatg
     7681 tggtcaaagg tagctatcgg gcgctggcaa aggaaatcgg ggcagaggtc gatagtggtg
     7741 gggcgctcaa gcacatacag gactgcatcg agcgcctttg gaaggtatcc atcatcgccc
     7801 agaatggccg caagcggcag gggtttcggc tgctgtcgga gtacgccagc gacgaggcgg
     7861 acgggcgcct gtacgtggcc ctgaacccct tgatcgcgca ggccgtcatg ggtggcggcc
     7921 agcatgtgcg catcagcatg gacgaggtgc gggcgctgga cagcgaaacc gcccgcctgc
     7981 tgcaccagcg gctgtgtggc tggatcgacc ccggcaaaac cggcaaggct tccatagata
     8041 ccttgtgcgg ctatgtctgg ccgtcagagg ccagtggttc gaccatgcgc aagcgccgca
     8101 agcgggtgcg cgaggcgttg ccggagctgg tcgcgctggg ctggacggta accgagttcg
     8161 cggcgggcaa gtacgacatc acccggccca aggcggcagg ctgacccccc ccactctatt
     8221 gtaaacaaga catttttatc ttttatattc aatggcttat tttcctgcta attggtaata
     8281 ccatgaaaaa taccatgctc agaaaaggct taacaatatt ttgaaaaatt gcctactgag
     8341 cgctgccgca cagctccata ggccgctttc ctggctttgc ttccagatgt atgctcttct
     8401 gctcccgaac gccagcaaga cgtagcccag cgcgtcggcc agcttgcaat tcgcgctaac
     8461 ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8521 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg
     8581 gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga
     8641 gagagttgca gcaagcggtc cacgtggttt gccccagcag gcgaaaatcc tgtttgatgg
     8701 tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga
     8761 tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct
     8821 gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt
     8881 gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat
     8941 tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg
     9001 ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc
     9061 gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa
     9121 gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca
     9181 gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt
     9241 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat
     9301 cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg
     9361 tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa
     9421 tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc
     9481 tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat
     9541 cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc
     9601 atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc aacgtaaatg ccgcttcgcc
     9661 ttcgcgcgcg aattgcaagc tgatccgggc ttatcgactg cacggtgcac caatgcttct
     9721 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa
     9781 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
     9841 ggttctggca aatattctga aatgagctg
//
