LOCUS       sequence-100305-        3102 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a TALEN protein specific for mouse ROSA26 locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Kasparek P, Krausova M, Haneckova R, Kriz V, Zbodakova O, Korinek V,
            Sedlacek R
  TITLE     Efficient gene targeting of the Rosa26 locus in mouse zygotes using 
            TALE nucleases.
  JOURNAL   FEBS Lett. 2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014.
  PUBMED    25241166
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014."
COMMENT     Sequence Label: TALEN-mROSA26 ELD
FEATURES             Location/Qualifiers
     source          1..3102
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      60..69
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             69..134
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             141..161
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcccagttc aattacagct cttaaggcta gagtacttaa tacgactcac tataggctag
       61 ccaccatgga ctataaggac cacgacggag actacaagga tcatgatatt gattacaaag
      121 acgatgacga taagatggcc ccaaagaaga agcggaaggt cggtatccac ggagtcccag
      181 cagccgtaga tttgagaact ttgggatatt cacagcagca gcaggaaaag atcaagccca
      241 aagtgaggtc gacagtcgcg cagcatcacg aagcgctggt gggtcatggg tttacacatg
      301 cccacatcgt agccttgtcg cagcaccctg cagcccttgg cacggtcgcc gtcaagtacc
      361 aggacatgat tgcggcgttg ccggaagcca cacatgaggc gatcgtcggt gtggggaaac
      421 agtggagcgg agcccgagcg cttgaggccc tgttgacggt cgcgggagag ctgagagggc
      481 ctccccttca gctggacacg ggccagttgc tgaagatcgc gaagcgggga ggagtcacgg
      541 cggtcgaggc ggtgcacgcg tggcgcaatg cactgacggg tgcccccctg aacctgaccc
      601 cggaccaagt ggtggctatc gccagcaaca atggcggcaa gcaagcgctc gaaacggtgc
      661 agcggctgtt gccggtgctg tgccaggacc atggcctgac tccggaccaa gtggtggcta
      721 tcgccagcca cgatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
      781 tgtgccagga ccatggcctg actccggacc aagtggtggc tatcgccagc cacgatggcg
      841 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
      901 tgactccgga ccaagtggtg gctatcgcca gccacgatgg cggcaagcaa gcgctcgaaa
      961 cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg
     1021 tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc
     1081 cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaaca
     1141 atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc
     1201 atggcctgac cccggaccaa gtggtggcta tcgccagcaa cattggcggc aagcaagcgc
     1261 tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc
     1321 aagtggtggc tatcgccagc aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc
     1381 tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca
     1441 gcaacaatgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc
     1501 aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc
     1561 aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc
     1621 cggaccaagt ggtggctatc gccagccacg atggcggcaa gcaagcgctc gaaacggtgc
     1681 agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta
     1741 tcgccagcaa cggtggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
     1801 tgtgccagga ccatggcctg actccggacc aagtggtggc tatcgccagc cacgatggcg
     1861 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
     1921 tgactccgga ccaagtggtg gctatcgcca gccacgatgg cggcaagcaa gcgctcgaaa
     1981 cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgactccg gaccaagtgg
     2041 tggctatcgc cagccacgat ggcggcaagc aagcgctcga aacggtgcag cggctgttgc
     2101 cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaaca
     2161 atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc
     2221 atggcctgac cccggaccaa gtggtggcta tcgccagcca cgatggcggc aagcaagcgc
     2281 tcgaaagcat tgtggcccag ctgagccggc ctgatccggc gttggccgcg ttgaccaacg
     2341 accacctcgt agcgctggcc tgcctcggcg gacgacccgc cttggatgcg gtgaagaagg
     2401 ggctcccgca cgcgcctgca ttgattaagc ggaccaacag aaggattccc gagaggacat
     2461 cacatcgagt ggcaggttcc caactcgtga agagtgaact tgaggagaaa aagtcggagc
     2521 tgcggcacaa attgaaatac gtaccgcatg aatacatcga acttatcgaa attgctagga
     2581 actcgactca agacagaatc cttgagatga aggtaatgga gttctttatg aaggtttatg
     2641 gataccgagg gaagcatctc ggtggatcac gaaaacccga cggagcaatc tatacggtgg
     2701 ggagcccgat tgattacgga gtgatcgtcg acacgaaagc ctacagcggt gggtacaatc
     2761 ttcccatcgg gcaggcagat gagatggaac gttatgtcga agaaaatcag accagggaca
     2821 aacacctcaa tccaaatgag tggtggaaag tgtatccttc atcagtgacc gagtttaagt
     2881 ttttgtttgt ctctgggcat ttcaaaggca actataaggc ccagctcaca cggttgaatc
     2941 acattacgaa ctgcaatggt gcggttttgt ccgtagagga actgctcatt ggtggagaaa
     3001 tgatcaaagc gggaactctg acactggaag aagtcagacg caagtttaac aatggcgaga
     3061 tcaatttccg ctcataatct agagcggccg cttcgagcag ac
//
