LOCUS       sequence-100308-        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pARv-RFP is a fluorescent reporter plasmid, that allows fast and 
            easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the 
            transfected cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Kasparek P, Krausova M, Haneckova R, Kriz V, Zbodakova O, Korinek V,
            Sedlacek R
  TITLE     Efficient gene targeting of the Rosa26 locus in mouse zygotes using 
            TALE nucleases.
  JOURNAL   FEBS Lett. 2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014.
  PUBMED    25241166
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014."
COMMENT     Sequence Label: pARv-RFP
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(457..475)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(467..513)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     misc_feature    547..991
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     primer_bind     complement(1039..1058)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gggataatcc tagcgaattc aggtttagtt cctggtgtac ttgaggggga tgagttcctc
       61 aatggtggtt ttgaccagct tgccattcat ctcaatgagc acaaagcagt caggagcata
      121 gtcagagatg agctctctgc acatgccaca ggggctgacc accctgatgg atctgtccac
      181 ctcatcagag taggggtgcc tgacagccac aatggtgtca aagtccttct gcccgttgct
      241 cacagcagac ccaatggcaa tggcttcagc acagacagtg accctgccaa tgtaggcctc
      301 aatgtggaca gcagagatga tctccccagt cttggtcctg atggccgccc cgacatggtg
      361 cttgttgtcc tcatagagca tggtgatctt ctcagtggcg acctccacca gctccagatc
      421 ctgctgagag atgttgaagg tcttcatggt ggccctccta tagtgagtcg tattatacta
      481 tgccgatata ctatgccgat gattaattgt caaacagtca gttgtattca tagggtcagt
      541 aattgcaaag gaaaggtgcc gacctccggt cacctattca ggcatagaag ctttttaaac
      601 cgagcgcttt tatagaagcc ccagtcccct tctcagatcc cgagtgtcgc gtgttacctc
      661 ggggtacctg aagggcatcc ttagcctgtc accagtgtgt gggtaccagt ttcaatgtca
      721 ctttaaagtg ggtttcgcag taaagtcgcc gtgctggggt tgcacacatg acgggcccgt
      781 gtgggcgtgg cttttggccc cgtgggtcct tgttaccaag gaggagttcc cacggccgtc
      841 atgctccgct acgaagcaac agtgctgtta ctaacactcg ccagtggatc gagcgtggag
      901 ccaaacgcag tacaaagtgt tacccctcta gacctggaaa gaccaggcgg agtttcaagt
      961 tgcacgtttt gtgtcattgg ggaaacctgc tcctagggtc gacaatcgat acgcgtggat
     1021 ccgatcaaaa gatacattga tgagtttgga caaaccacaa ctagaatgca
//
