LOCUS       sequence-100309-        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pARv-RFP is a fluorescent reporter plasmid, that allows fast and 
            easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the 
            transfected cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Kasparek P, Krausova M, Haneckova R, Kriz V, Zbodakova O, Korinek V,
            Sedlacek R
  TITLE     Efficient gene targeting of the Rosa26 locus in mouse zygotes using 
            TALE nucleases.
  JOURNAL   FEBS Lett. 2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014.
  PUBMED    25241166
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014."
COMMENT     Sequence Label: pARv-RFP
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(158..177)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    187..268
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     212..231
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cagtggatcg agcgtggagc caaacgcagt acaaagtgtt acccctctag acctggaaag
       61 accaggcgga gtttcaagtt gcacgttttg tgtcattggg gaaacctgct cctagggtcg
      121 acaatcgata cgcgtggatc cgatcaaaag atacattgat gagtttggac aaaccacaac
      181 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      241 agccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
      301 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
      361 ggctgattat gacacccggg ttatctgtgc cccagtttgc tagggaggtc gcagtacttg
      421 gccacagcca tctcgtgctg ctcgacgtag gtctctttgt cggcctcctt gattctttcc
      481 agtctgtggt ccacgaagtg gaagccgggc atcttgaggt tcttagcggg tttcttggat
      541 ctgtatgtgg tcttgaagga gcagtgcagg tagcccccgc ccacgagctt cagggccatc
      601 tggctgtggc ctctcaggcc gccgtcagcg gggtacagca tctcggtgtt ggcctcccag
      661 ccgcgtgttt tcttctgcat cacagggccg ttggatggga agttcacccc gttgatcttg
      721 acgttgtaga tgatgcagcc gttctggaag ctggtgtcct gggtagcggt cagcacgccc
      781 ccgtcttcgt atgtggtgat tctctcccat gtgaagccct cagggaagga ctgcttaaag
      841 aagtcgggga tgccctgggt gtggttgatg aaggctttgc tgccgtacat gaagctggta
      901 gccaggatgt cgaaggcgaa ggggagaggg ccgccctcga ccaccttgat cttcatggtc
      961 tgggtgccct cgtagggctt gccttcgccc tcggatgtgc acttgaagtg gtggttgttc
     1021 acggtgccct ccatgtacag cttcatgtgc atg
//
