LOCUS       sequence-10031-e       10679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Charpentier X, Gabay JE, Reyes M, Zhu JW, Weiss A, Shuman HA
  TITLE     Chemical genetics reveals bacterial and host cell functions critical
            for type IV effector translocation by Legionella pneumophila.
  JOURNAL   PLoS Pathog. 2009 Jul . 5(7):e1000501.
  PUBMED    19578436
REFERENCE   2  (bases 1 to 10679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Jul . 5(7):e1000501."
COMMENT     Sequence Label: pXDC61-FabI
FEATURES             Location/Qualifiers
     source          1..10679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..22,23..29)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    37..53
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     42..64
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             101..886
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     primer_bind     complement(247..266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(1763..1780)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1933..2019
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2111..2138
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2157..2248
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2699..2801
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2802..3461
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2868..2887)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3793..4187
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            4529..4616
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             5855..6826
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDAPTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGHDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             7337..8176
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             8163..9014
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRKRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     10334..10353
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10373..10450)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca
       61 caggaaacag aattcagaag gagatataca tatggctcac ccagaaacgc tggtgaaagt
      121 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      181 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      241 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      301 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      361 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      421 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      481 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      541 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
      601 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      661 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      721 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      781 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
      841 aaatagacag atcgctgaga taggtgcctc actgattaag cattggggaa gcggtaccat
      901 gggaggagat actattgtgg gatttttaac tggaaaaaaa gcacttattg ttggattggc
      961 aagcaacaga tcgattgcat atggcattgc taaagcattc cataatcaag gcgctgagtt
     1021 ggcttttact taccaaaacg aaaaattaca gtcacgtgtt gaaacaatgg catcggaatt
     1081 taattcaact ctagtttttc cttgtgatgt tgcaagtgat gaagaaataa aagccgtgtt
     1141 tgataatttg cgtaatcact gggataaatt agatatcctg gtccactctg tagcttatgc
     1201 tcctgctgat cagatcagtg gtgattttgt agaatgtgca aatagagaag gatttagaat
     1261 agctcatgat attagtgcct atagcctcat aggtttgtct caagccgcat tgcctatgat
     1321 gctagatact cagggttcaa ttttaacctt aagctattat ggggcagaaa aagccgtacc
     1381 aaactataac gttatgggtg ttgcgaaggc aagcttggaa gcctctgtgc gctatctggc
     1441 agccagctta ggatccagag gattaagaat taatgccata tcagctggac caattaaaac
     1501 tcttgccgct gctggaatta aagattttcg taaaattcat gctgcctatg caaacattac
     1561 tccattacaa agaaatgtta ctgctgatga agtcggaaat actgctgcct tcttatgctc
     1621 tgatctggca tccgggatta ctggtgaagt gctacatgtt gatgcaggat atcacgcagt
     1681 atcagcgatg agcgaactag gtggtacccg gggatcctct agataagctt ggctgttttg
     1741 gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga
     1801 taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
     1861 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
     1921 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1981 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     2041 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
     2101 caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg
     2161 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2221 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     2281 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     2341 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     2401 cggtaagatc cttgagagtt ttcgccccga agaacgtgat gtccggcggt gcttttgccg
     2461 ttacgcacca ccccgtcagt agctgaacag gagggacagc tgatagaaac agaagccact
     2521 ggagcaagca cctcaaaaac accatcatac actaaatcag taagttggca gcatcacccg
     2581 acgcactttg cgccgaataa atacctgtga cggaagatca cttcgcagaa taaataaatc
     2641 ctggtgtccc tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg
     2701 atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat
     2761 tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     2821 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     2881 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     2941 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     3001 tgaatgctca tccggaatta cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     3061 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     3121 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     3181 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     3241 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     3301 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     3361 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     3421 aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
     3481 gtcttcaaat tcccgttgca catagcccgg caattccttt ccctgctctg ccataagcgc
     3541 agcgaatgcc gggtaatact cgtcaacgat ctgatagaga agggtttgct cgggtcggtg
     3601 gctctggtaa cgaccagtat cccgatcccg gctggccgtc ctggccgcca catgaggcat
     3661 gttccgcgtc cttgcaatac tgtgtttaca tacagtctat cgcttagcgg aaagttcttt
     3721 taccctcagc cgaaatgcct gccgttgcta gacattgcca gccagtgccc gtcactcccg
     3781 tactaactgt cacgaacccc tgcaataact gtcacgcccc cctgcaataa ctgtcacgaa
     3841 cccctgcaat aactgtcacg cccccaaacc tgcaaaccca gcaggggcgg gggctggcgg
     3901 ggtgttggaa aaatccatcc atgattatct aagaataatc cactaggcgc ggttatcagc
     3961 gcccttgtgg ggcgctgctg cccttgccca atatgcccgg ccagaggccg gatagctggt
     4021 ctattcgctg cgctaggcta cacaccgccc caccgctgcg cggcaggggg aaaggcgggc
     4081 aaagcccgct aaaccccaca ccaaaccccg cagaaatacg ctggagcgct tttagccgct
     4141 ttagcggcct ttccccctac ccgaagggtg ggggcgcgtg tgcagccccg cagggcctgt
     4201 ctcggtcgat cattcagccc ggctcatcct tctggcgtgg cggcagaccg aacaaggcgc
     4261 ggtcgtggtc gcgttcaagg tacgcatcca ttgccgccat gagccgatcc tccggccact
     4321 cgctgctgtt caccttggcc aaaatcatgg cccccaccag caccttgcgc cttgtttcgt
     4381 tcttgcgctc ttgctgctgt tcccttgccc gctcccgctg aatttcggca ttgattcgcg
     4441 ctcgttgttc ttcgagcttg gccagccgat ccgccgcctt gttgctcccc ttaaccatct
     4501 tgacacccca ttgttaatgt gctgtctcgt aggctatcat ggaggcacag cggcggcaat
     4561 cccgacccta ctttgtaggg gagggcgcac ttaccggttt ctcttcgaga aactggccta
     4621 acggccaccc ttcgggcggt gcgctctccg agggccattg catggagccg aaaagcaaaa
     4681 gcaacagcga ggcagcatgg cgatttatca ccttacggcg aaaaccggca gcaggtcggg
     4741 cggccaatcg gccagggcca aggccgacta catccagcgc gaaggcaagt atgcccgcga
     4801 catggatgaa gtcttgcacg ccgaatccgg gcacatgccg gagttcgtcg agcggcccgc
     4861 cgactactgg gatgctgccg acctgtatga acgcgccaat gggcggctgt tcaaggaggt
     4921 cgaatttgcc ctgccggtcg agctgaccct cgaccagcag aaggcgctgg cgtccgagtt
     4981 cgcccagcac ctgaccggtg ccgagcgcct gccgtatacg ctggccatcc atgccggtgg
     5041 cggcgagaac ccgcactgcc acctgatgat ctccgagcgg atcaatgacg gcatcgagcg
     5101 gcccgccgct cagtggttca agcggtacaa cggcaagacc ccggagaagg gcggggcaca
     5161 gaagaccgaa gcgctcaagc ccaaggcatg gcttgagcag acccgcgagg catgggccga
     5221 ccatgccaac cgggcattag agcgggctgg ccacgacgcc cgcattgacc acagaacact
     5281 tgaggcgcag ggcatcgagc gcctgcccgg tgttcacctg gggccgaacg tggtggagat
     5341 ggaaggccgg ggcatccgca ccgaccgggc agacgtggcc ctgaacatcg acaccgccaa
     5401 cgcccagatc atcgacttac aggaataccg ggaggcaata gaccatgaac gcaatcgaca
     5461 gagtgaagaa atccagaggc atcaacgagt tagcggagca gatcgaaccg ctggcccaga
     5521 gcatggcgac actggccgac gaagcccggc aggtcatgag ccagaccaag caggccagcg
     5581 aggcgcaggc ggcggagtgg ctgaaagccc agcgccagac aggggcggca tgggtggagc
     5641 tggccaaaga gttgcgggag gtagccgccg aggtgagcag cgccgcgcag agcgcccgga
     5701 gcgcgtcgcg ggggtggcac tggaagctat ggctaaccgt gatgctggct tccatgatgc
     5761 ctacggtggt gctgctgatc gcatcgttgc tcttgctcga cctgacgcca ctgacaaccg
     5821 aggacggctc gatctggctg cgcttggtgg cccgatgaag aacgacagga ctttgcaggc
     5881 cataggccga cagctcaagg ccatgggctg tgagcgcttc gatatcggcg tcagggacgc
     5941 acccaccggc cagatgatga accgggaatg gtcagccgcc gaagtgctcc agaacacgcc
     6001 atggctcaag cggatgaatg cccagggcaa tgacgtgtat atcaggcccg ccgagcagga
     6061 gcggcatggt ctggtgctgg tggacgacct cagcgagttt gacctggatg acatgaaagc
     6121 cgagggccgg gagcctgccc tggtagtgga aaccagcccg aagaactatc aggcatgggt
     6181 caaggtggcc gacgccgcag gcggtgaact tcgggggcag attgcccgga cgctggccag
     6241 cgagtacgac gccgacccgg ccagcgccga cagccgccac tatggccgct tggcgggctt
     6301 caccaaccgc aaggacaagc acaccacccg cgccggttat cagccgtggg tgctgctgcg
     6361 tgaatccaag ggcaagaccg ccaccgctgg cccggcgctg gtgcagcagg ctggccagca
     6421 gatcgagcag gcccagcggc agcaggagaa ggcccgcagg ctggccagcc tcgaactgcc
     6481 cgagcggcag cttagccgcc accggcgcac ggcgctggac gagtaccgca gcgagatggc
     6541 cgggctggtc aagcgcttcg gtcatgacct cagcaagtgc gactttatcg ccgcgcagaa
     6601 gctggccagc cggggccgca gtgccgagga aatcggcaag gccatggccg aggccagccc
     6661 agcgctggca gagcgcaagc ccggccacga agcggattac atcgagcgca ccgtcagcaa
     6721 ggtcatgggt ctgcccagcg tccagcttgc gcgggccgag ctggcacggg caccggcacc
     6781 ccgccagcga ggcatggaca ggggcgggcc agatttcagc atgtagtgct tgcgttggta
     6841 ctcacgcctg ttatactatg agtactcacg cacagaaggg ggttttatgg aatacgaaaa
     6901 aagcgcttca gggtcggtct acctgatcaa aagtgacaag ggctattggt tgcccggtgg
     6961 ctttggttat acgtcaaaca aggccgaggc tggccgcttt tcagtcgctg atatggccag
     7021 ccttaacctt gacggctgca ccttgtcctt gttccgcgaa gacaagcctt tcggccccgg
     7081 caagtttctc ggtgactgat atgaaagacc aaaaggacaa gcagaccggc gacctgctgg
     7141 ccagccctga cgctgtacgc caagcgcgat atgccgagcg catgaaggcc aaagggatgc
     7201 gtcagcgcaa gttctggctg accgacgacg aatacgaggc gctgcgcgag tgcctggaag
     7261 aactcagagc ggcgcagggc gggggtagtg accccgccag cgcctaacca ccaactgcct
     7321 gcaaaggagg caatcaatgg ctacccataa gcctatcaat attctggagg cgttcgcagc
     7381 agcgccgcca ccgctggact acgttttgcc caacatggtg gccggtacgg tcggggcgct
     7441 ggtgtcgccc ggtggtgccg gtaaatccat gctggccctg caactggccg cacagattgc
     7501 aggcgggccg gatctgctgg aggtgggcga actgcccacc ggcccggtga tctacctgcc
     7561 cgccgaagac ccgcccaccg ccattcatca ccgcctgcac gcccttgggg cgcacctcag
     7621 cgccgaggaa cggcaagccg tggctgacgg cctgctgatc cagccgctga tcggcagcct
     7681 gcccaacatc atggccccgg agtggttcga cggcctcaag cgcgccgccg agggccgccg
     7741 cctgatggtg ctggacacgc tgcgccggtt ccacatcgag gaagaaaacg ccagcggccc
     7801 catggcccag gtcatcggtc gcatggaggc catcgccgcc gataccgggt gctctatcgt
     7861 gttcctgcac catgccagca agggcgcggc catgatgggc gcaggcgacc agcagcaggc
     7921 cagccggggc agctcggtac tggtcgataa catccgctgg cagtcctacc tgtcgagcat
     7981 gaccagcgcc gaggccgagg aatggggtgt ggacgacgac cagcgccggt tcttcgtccg
     8041 cttcggtgtg agcaaggcca actatggcgc accgttcgct gatcggtggt tcaggcggca
     8101 tgacggcggg gtgctcaagc ccgccgtgct ggagaggcag cgcaagagca agggggtgcc
     8161 ccgtggtgaa gcctaagaac aagcacagcc tcagccacgt ccggcacgac ccggcgcact
     8221 gtctggcccc cggcctgttc cgtgccctca agcggggcga gcgcaagcgc agcaagctgg
     8281 acgtgacgta tgactacggc gacggcaagc ggatcgagtt cagcggcccg gagccgctgg
     8341 gcgctgatga tctgcgcatc ctgcaagggc tggtggccat ggctgggcct aatggcctag
     8401 tgcttggccc ggaacccaag accgaaggcg gacggcagct ccggctgttc ctggaaccca
     8461 agtgggaggc cgtcaccgct gaatgccatg tggtcaaagg tagctatcgg gcgctggcaa
     8521 aggaaatcgg ggcagaggtc gatagtggtg gggcgctcaa gcacatacag gactgcatcg
     8581 agcgcctttg gaaggtatcc atcatcgccc agaatggccg caagcggcag gggtttcggc
     8641 tgctgtcgga gtacgccagc gacgaggcgg acgggcgcct gtacgtggcc ctgaacccct
     8701 tgatcgcgca ggccgtcatg ggtggcggcc agcatgtgcg catcagcatg gacgaggtgc
     8761 gggcgctgga cagcgaaacc gcccgcctgc tgcaccagcg gctgtgtggc tggatcgacc
     8821 ccggcaaaac cggcaaggct tccatagata ccttgtgcgg ctatgtctgg ccgtcagagg
     8881 ccagtggttc gaccatgcgc aagcgccgca agcgggtgcg cgaggcgttg ccggagctgg
     8941 tcgcgctggg ctggacggta accgagttcg cggcgggcaa gtacgacatc acccggccca
     9001 aggcggcagg ctgacccccc ccactctatt gtaaacaaga catttttatc ttttatattc
     9061 aatggcttat tttcctgcta attggtaata ccatgaaaaa taccatgctc agaaaaggct
     9121 taacaatatt ttgaaaaatt gcctactgag cgctgccgca cagctccata ggccgctttc
     9181 ctggctttgc ttccagatgt atgctcttct gctcccgaac gccagcaaga cgtagcccag
     9241 cgcgtcggcc agcttgcaat tcgcgctaac ttacattaat tgcgttgcgc tcactgcccg
     9301 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9361 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
     9421 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgtggttt
     9481 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt
     9541 cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg
     9601 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa
     9661 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc
     9721 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca
     9781 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac
     9841 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac
     9901 tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag
     9961 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc
    10021 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca
    10081 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt
    10141 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc
    10201 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca
    10261 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct
    10321 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca
    10381 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt
    10441 cgatggtgtc aacgtaaatg ccgcttcgcc ttcgcgcgcg aattgcaagc tgatccgggc
    10501 ttatcgactg cacggtgcac caatgcttct ggcgtcaggc agccatcgga agctgtggta
    10561 tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct
    10621 ggataatgtt ttttgcgccg acatcataac ggttctggca aatattctga aatgagctg
//
