LOCUS       sequence-100310-        1085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pARv-RFP is a fluorescent reporter plasmid, that allows fast and 
            easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the 
            transfected cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Kasparek P, Krausova M, Haneckova R, Kriz V, Zbodakova O, Korinek V,
            Sedlacek R
  TITLE     Efficient gene targeting of the Rosa26 locus in mouse zygotes using 
            TALE nucleases.
  JOURNAL   FEBS Lett. 2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014.
  PUBMED    25241166
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014."
COMMENT     Sequence Label: pARv-RFP
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             380..1072
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKVV
                     EGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTAT
                     QDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALKL
                     VGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYCD
                     LPSKLGHR"
ORIGIN
        1 ggtatctttt aaaccctttt ttaggtgttg tgaaaaccac cgctaattca aagcaaccgg
       61 tgatcgccac catgagcgag ctgatcaagg agaacatgca catgaagctg tacatggagg
      121 gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg
      181 gcacccagac catgaagatc aaggtggtcg agggcggccc tctccccttc gccttcgaca
      241 tcctggctac cagcttcatg tacggcagca aagccttcat caaccacacc cagggcatcc
      301 ccgacttctt taagcagtcc ttccctgatt cgccaccgct agataatgat agttaatatt
      361 gatatcatga cgatcgacta gcgagctgat caaggagaac atgcacatga agctgtacat
      421 ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta
      481 cgagggcacc cagaccatga agatcaaggt ggtcgagggc ggccctctcc ccttcgcctt
      541 cgacatcctg gctaccagct tcatgtacgg cagcaaagcc ttcatcaacc acacccaggg
      601 catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga gaatcaccac
      661 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcttccaga acggctgcat
      721 catctacaac gtcaagatca acggggtgaa cttcccatcc aacggccctg tgatgcagaa
      781 gaaaacacgc ggctgggagg ccaacaccga gatgctgtac cccgctgacg gcggcctgag
      841 aggccacagc cagatggccc tgaagctcgt gggcgggggc tacctgcact gctccttcaa
      901 gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggct tccacttcgt
      961 ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga
     1021 gatggctgtg gccaagtact gcgacctccc tagcaaactg gggcacagat aacccgggtg
     1081 tcata
//
