LOCUS       sequence-100311-        7743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pARv-RFP is a fluorescent reporter plasmid, that allows fast and 
            easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the 
            transfected cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Kasparek P, Krausova M, Haneckova R, Kriz V, Zbodakova O, Korinek V,
            Sedlacek R
  TITLE     Efficient gene targeting of the Rosa26 locus in mouse zygotes using 
            TALE nucleases.
  JOURNAL   FEBS Lett. 2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014.
  PUBMED    25241166
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014."
COMMENT     Sequence Label: pARv-RFP
FEATURES             Location/Qualifiers
     source          1..7743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        9..312
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          700..1644
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1601..1621
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(1719..1721,1722..1724,1725..2435)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNAPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1764..1785)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2025..2044)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2372..2393
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2504..2625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2813..3401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3302..3321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3523..3714
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3546..3566)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          3921..4868
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     CDS             5224..5916
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKVV
                     EGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTAT
                     QDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALKL
                     VGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYCD
                     LPSKLGHR"
     polyA_signal    6029..6110
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6187..6631
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        6665..6711
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        6703..6721
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    7170..7742
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcaggcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
       61 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      121 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      181 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      241 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      301 gctattacca tgatgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      361 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttgactagtc
      421 agggccccaa cccccccaag cccccatttc acaacacgct ggcgctacag gcgcgtgact
      481 tccccttgct ttggggcggg gggctgagac tcctatgtgc tccggattgg tcaggcacgg
      541 ccttcggccc cgcctcctgc caccgcagat tggccgctag gcctccccga gcgccctgcc
      601 tccgagggcc ggcgcaccat aaaagaagcc gccctagcca cgtcccctcg cagttcggcg
      661 gtcccgcggg tctgtctcaa gcttgccgcc agaacacagg taagtgccgt gtgtggttcc
      721 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccatgccc
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcttgggcg
      901 ctggggccgc cgcgtgctaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgctaa
      961 gtctctagcc atttaaaatt tttgataacc agctgcgacg ctttttttct ggcgagatag
     1021 tcttgtaaat gcgggccagg atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     1141 accgagaatc ggacgggggt agtctcaaac tggccggcct gctctggtgc ctggcctcgc
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1321 cccgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cttcctcatc
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttgtc
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1501 ccacactgag tgggtggaga ctgaagagtt aggccagctt ggcacttgat gtaattctcc
     1561 ttggaatttg ccctttttga gtttggatct tgcctcattc tcaagcctca gacagtggtt
     1621 caaagttttt ttcttccatt tcaggtgtcg tgaaaactac ccctaaaagc caccggcgtg
     1681 cgcaagatct gaattcttcg aactcgaggc ccgggaccat ggtgagcaag ggcgaggagc
     1741 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1801 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1861 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     1921 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1981 ctatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     2041 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     2101 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     2161 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     2221 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacgccc
     2281 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     2341 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     2401 ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct cgactagcta
     2461 gtagctagac gcgtcccggg gaattctagc tggccagaca tgataagata cattgatgag
     2521 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     2581 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     2641 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     2701 ctctacaaat gtggtatgga aatgttaatt aactagccat gaccaaaatc ccttaacgtg
     2761 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2821 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2881 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2941 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3001 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3061 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3121 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3181 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3241 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3301 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3361 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3421 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttcttaatt aacctgcagg
     3481 gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggct gaaagaacca
     3541 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     3601 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     3661 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccactagt
     3721 tccgccagag cgcgcgaggg cctccagcgg ccgcccctcc cccacagcag gggcggggtc
     3781 ccgcgcccac cggaaggagc gggctcgggg cgggcggcgc tgattggccg gggcgggcct
     3841 gacgccgacg cggctataag agaccacaag cgacccgcag ggccagacgt tcttcgccga
     3901 agcttgccgt cagaacgcag gtgaggggcg ggtgtggctt ccgcgggccg ccgagctgga
     3961 ggtcctgctc cgagcgggcc gggccccgct gtcgtcggcg gggattagct gcgagcattc
     4021 ccgcttcgag ttgcgggcgg cgcgggaggc agagtgcgag gcctagcggc aaccccgtag
     4081 cctcgcctcg tgtccggctt gaggcctagc gtggtgtccg cgccgccgcc gcgtgctact
     4141 ccggccgcac tctggtcttt tttttttttg ttgttgttgc cctgctgcct tcgattgccg
     4201 ttcagcaata ggggctaaca aagggagggt gcggggcttg ctcgcccgga gcccggagag
     4261 gtcatggttg gggaggaatg gagggacagg agtggcggct ggggcccgcc cgccttcgga
     4321 gcacatgtcc gacgccacct ggatggggcg aggcctgggg tttttcccga agcaaccagg
     4381 ctggggttag cgtgccgagg ccatgtggcc ccagcacccg gcacgatctg gcttggcggc
     4441 gccgcgttgc cctgcctccc taactagggt gaggccatcc cgtccggcac cagttgcgtg
     4501 cgtggaaaga tggccgctcc cgggccctgt tgcaaggagc tcaaaatgga ggacgcggca
     4561 gcccggtgga gcgggcgggt gagtcaccca cacaaaggaa gagggcctgg tccctcaccg
     4621 gctgctgctt cctgtgaccc cgtggtccta tcggccgcaa tagtcacctc gggcttttga
     4681 gcacggctag tcgcggcggg gggaggggat gtaatggcgt tggagtttgt tcacatttgg
     4741 tgggtggaga ctagtcaggc cagcctggcg ctggaagtca tttttggaat ttgtcccctt
     4801 gagttttgag cggagctaat tctcgggctt cttagcggtt caaaggtatc ttttaaaccc
     4861 ttttttaggt gttgtgaaaa ccaccgctaa ttcaaagcaa ccggtgatcg ccaccatgag
     4921 cgagctgatc aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca
     4981 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgaa
     5041 gatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
     5101 catgtacggc agcaaagcct tcatcaacca cacccagggc atccccgact tctttaagca
     5161 gtccttccct gattcgccac cgctagataa tgatagttaa tattgatatc atgacgatcg
     5221 actagcgagc tgatcaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac
     5281 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     5341 atgaagatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
     5401 agcttcatgt acggcagcaa agccttcatc aaccacaccc agggcatccc cgacttcttt
     5461 aagcagtcct tccctgaggg cttcacatgg gagagaatca ccacatacga agacgggggc
     5521 gtgctgaccg ctacccagga caccagcttc cagaacggct gcatcatcta caacgtcaag
     5581 atcaacgggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac acgcggctgg
     5641 gaggccaaca ccgagatgct gtaccccgct gacggcggcc tgagaggcca cagccagatg
     5701 gccctgaagc tcgtgggcgg gggctacctg cactgctcct tcaagaccac atacagatcc
     5761 aagaaacccg ctaagaacct caagatgccc ggcttccact tcgtggacca cagactggaa
     5821 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgagatggc tgtggccaag
     5881 tactgcgacc tccctagcaa actggggcac agataacccg ggtgtcataa tcagccatac
     5941 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     6001 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggctacaa
     6061 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6121 tggtttgtcc aaactcatca atgtatcttt tgatcggatc cacgcgtatc gattgtcgac
     6181 cctaggagca ggtttcccca atgacacaaa acgtgcaact tgaaactccg cctggtcttt
     6241 ccaggtctag aggggtaaca ctttgtactg cgtttggctc cacgctcgat ccactggcga
     6301 gtgttagtaa cagcactgtt gcttcgtagc ggagcatgac ggccgtggga actcctcctt
     6361 ggtaacaagg acccacgggg ccaaaagcca cgcccacacg ggcccgtcat gtgtgcaacc
     6421 ccagcacggc gactttactg cgaaacccac tttaaagtga cattgaaact ggtacccaca
     6481 cactggtgac aggctaagga tgcccttcag gtaccccgag gtaacacgcg acactcggga
     6541 tctgagaagg ggactggggc ttctataaaa gcgctcggtt taaaaagctt ctatgcctga
     6601 ataggtgacc ggaggtcggc acctttcctt tgcaattact gaccctatga atacaactga
     6661 ctgtttgaca attaatcatc ggcatagtat atcggcatag tataatacga ctcactatag
     6721 gagggccacc atgaagacct tcaacatctc tcagcaggat ctggagctgg tggaggtcgc
     6781 cactgagaag atcaccatgc tctatgagga caacaagcac catgtcgggg cggccatcag
     6841 gaccaagact ggggagatca tctctgctgt ccacattgag gcctacattg gcagggtcac
     6901 tgtctgtgct gaagccattg ccattgggtc tgctgtgagc aacgggcaga aggactttga
     6961 caccattgtg gctgtcaggc acccctactc tgatgaggtg gacagatcca tcagggtggt
     7021 cagcccctgt ggcatgtgca gagagctcat ctctgactat gctcctgact gctttgtgct
     7081 cattgagatg aatggcaagc tggtcaaaac caccattgag gaactcatcc ccctcaagta
     7141 caccaggaac taaacctgaa ttcgctagga ttatccctaa tacctgccac cccactctta
     7201 atcagtggtg gaagaacggt ctcagaactg tttgtttcaa ttggccattt aagtttagta
     7261 gtaaaagact ggttaatgat aacaatgcat cgtaaaacct tcagaaggaa aggagaatgt
     7321 tttgtggacc actttggttt tcttttttgc gtgtggcagt tttaagttat tagtttttaa
     7381 aatcagtact ttttaatgga aacaacttga ccaaaaattt gtcacagaat tttgagaccc
     7441 attaaaaaag ttaaatgaga aacctgtgtg ttcctttggt caacaccgag acatttaggt
     7501 gaaagacatc taattctggt tttacgaatc tggaaacttc ttgaaaatgt aattcttgag
     7561 ttaacacttc tgggtggaga atagggttgt tttcccccca cataattgga aggggaagga
     7621 atatcattta aagctatggg agggttgctt tgattacaac actggagaga aatgcagcat
     7681 gttgctgatt gcctgtcact aaaacaggcc aaaaactgag tccttgggtt gcatagaaag
     7741 ctg
//
