LOCUS       sequence-10032-e       13313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13313)
  AUTHORS   Charpentier X, Gabay JE, Reyes M, Zhu JW, Weiss A, Shuman HA
  TITLE     Chemical genetics reveals bacterial and host cell functions critical
            for type IV effector translocation by Legionella pneumophila.
  JOURNAL   PLoS Pathog. 2009 Jul . 5(7):e1000501.
  PUBMED    19578436
REFERENCE   2  (bases 1 to 13313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Jul . 5(7):e1000501."
COMMENT     Sequence Label: pXDC61-LepA
FEATURES             Location/Qualifiers
     source          1..13313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..22,23..29)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    37..53
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     42..64
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             101..886
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     primer_bind     complement(247..266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(4397..4414)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4567..4653
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4745..4772
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4791..4882
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5333..5435
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             5436..6095
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5502..5521)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      6427..6821
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            7163..7250
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             8489..9460
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDAPTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGHDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             9971..10810
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             10797..11648
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRKRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     12968..12987
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(13007..13084)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca
       61 caggaaacag aattcagaag gagatataca tatggctcac ccagaaacgc tggtgaaagt
      121 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      181 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      241 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      301 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      361 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      421 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      481 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      541 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
      601 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      661 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      721 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      781 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
      841 aaatagacag atcgctgaga taggtgcctc actgattaag cattggggaa gcggtaccat
      901 gaacgaaatc atactgcact taatgaatga aatctctacc ttgactccac caattaaccc
      961 cttaaattta aagcaaatat ttagttcaac cctactgccg gaaggaaaag ctcctgttga
     1021 agttgaatcc cagttaaatc ttttattgtt aattaaaaaa tatgttgatg aatattccag
     1081 tttagctgaa gaacaagaag agtataaaag aaaaaaacac aaactagatg aggtcatgaa
     1141 aaaaggagaa gaactgcaaa gaaaaatttc tgatttggct aaaaaaattg agcacgcaaa
     1201 aaatgaatta aatcctttat acaaagaaat cgagcctaaa gcgaacgaag tcctgtccaa
     1261 acaaaatcca ttagcgcctc cttataaatt ccctgaatac ggagaggttc aaaaatcaat
     1321 cgtggttggg cactacagtt atctcttcaa aaccagtaag gaaatagaaa ctacacgtgc
     1381 caaattaaag ctggttttgc tgaaaaataa atggaatgcc ttaatggcag atttgtctca
     1441 gttcggcatt caaccccctg tggaagaaga tgctgttgat agtttacaaa aatttatagc
     1501 tgcagttgaa ttggaaatta aaaaattaac tcgcgcttgt gaaaaatggg atgaattgca
     1561 aaagcgattc gccaaacaat tatctgcaac tgatattaat aaaaaagaga ctgagctaga
     1621 aaaacaaatt gaactattgc ttaaagacct gggtcgtatt gaaaccgtag taactaacca
     1681 ctccctggca cccgatttaa aagcagagtt aaccaggaaa tttcaagaaa gtgaagacac
     1741 tcaaggattg atacaggaat accaaaacca gattgatgga ttattatcta attttaaccc
     1801 atcttcctgg ctttcatggt attcggattc taattattct gataatcagc aaaaacttaa
     1861 gaattcagtt agcttcctgc aattattagc tcaacaaaaa gagctaaaaa tcaaacatca
     1921 tgaacttgtg aaacagcaag aactgttgat taaagcgata ccagaaactc cttcaaatga
     1981 agaggatata tctatttata aaaaattagt tcctgatgcc attgacctga taaatgaaat
     2041 accaatagaa agtataccaa attttgtttt accaagtggc ctttcttatg actcttcgcc
     2101 ggccgatttt tatttggtac ttctcattct catgccaaaa gtttcggaaa agaaggaaca
     2161 attttcacaa gcgttggaaa aacttcaaga aatgcctgct ctgggcaaac aaatacaaca
     2221 actaagaagt ggatacgatt tacctataac tatggatgta caacttccaa gtttgcaaga
     2281 agctgaagaa agtaaagatt cattgaatga agaggatcca cttaaagaag agttaaaaag
     2341 ccagattaaa ctatgccaat cttatcttga aacagccaag caaattgatt tgcttatgaa
     2401 agatcatgga caaacaacca atgaagcaaa agcacttatt gagcaattac gtttaatttc
     2461 aaatcaacca ctaaagaacg aagacataaa ctctattaaa gaagatttat ctactcttgc
     2521 caatcaaatc caatcccttg tgtctgaatt aaatcaatta cctttgccta atctgtccgg
     2581 tgaaaaaatt gatcctccat tggaaaaaca ggaaatagca gtagataaaa caatccctct
     2641 tactgtggaa ttgatgcacc ctgcccaagt aattcagata ggcaatgatt ctcccataga
     2701 aaaacagcag ctagacaatg aggaagaaac gccggttgtt ctggaatcat tacgcccccg
     2761 tccagtcatt cataaggaat ctgaatctat cacccaaaaa ctgcaagtag acaatggaca
     2821 agaaatgccg gttgttctgg attcaacatg cactcctcaa gccattcata aggaacttga
     2881 atctaccact gaaaaactgc aagtagacaa tggacacgaa ataccggttg ttctcgagtc
     2941 aacacgctct cctcaaatca ttcatactga gtctgaatct gttggtaaaa aacagcatgt
     3001 agaagtagag caagagatac cagtcacact ggaattaata cgaccctctt ctatggttga
     3061 aaaggattcc gtggctactg tagaaaaaca gcaagtaact gagagacagg aaacaccaat
     3121 cgtccttgaa tcaacacgac tctctcctat ggttcagaaa gacactgggc tttctggcga
     3181 aaagcaacat aaagagatag aacaagaaat atctgtcgtt acggaattaa caccccacac
     3241 ccaagtggtt cggcaaggct ctgagtccag tttagaacaa caagtaccca acggacaaga
     3301 aacactggct gttgtcaaat caagcccatc ggaaccattg agaccatcaa cccctgaagt
     3361 tccagctata tccaggaaac caaatggctt gtcattattt aatggtcacg atgaattatc
     3421 agaagataat attttagcct tttttgatga ggctggtaat caaattagca tctcatctga
     3481 agaagattct gagacgttta ccgtagacag gggaaaagta atttcctcag ggattgataa
     3541 agcatttaaa gaaaagcctg tttctacagg ccatgagtta gataaattat caaatcctca
     3601 acaccaggaa tctgccccta gcatctcccc tttaccacca tcttctatcc tgcttaagca
     3661 aaaattagat tcatttcatg tacaaaacat ggaatatata aagcaacatt cagaagaaat
     3721 acagctatgg tataaaggtt tatatgatgc agcgcaaagt tcttgtgtaa atgaggcatt
     3781 gggtcttaaa gcacttcatc ttttaaaaga catcttgttt gaattaaaaa atcaaaatga
     3841 tttatctgta ttactagctt acaagcgcat gtgcccaaac ccattgcaag atatacaaaa
     3901 tattctccgc ttaaaacctg ctctgcctat agttgatgaa tcgattgatg aagaacagca
     3961 attaaagaat tggcctgaag aattgcaaaa atttcatcag caatatgtaa aattgaaaaa
     4021 ggaacatcca ttggaagctg aattatttat tcaggcaatc cattcactta tttcaattaa
     4081 acatttgatg gaactccctg atgcaaaaac ttcaaaccga gaagccatgc ctctcattac
     4141 tcaagatccc cgatatgaac ctttaaaacg gcatagagga tttataagag catgggaata
     4201 tattgaagat tttttccgaa tgttaatagg taagttaaca gggcaagatg aatatgagta
     4261 ttcaaaaagg ccttgttttt tcaaaacaag atctcaccga ttacttgaag aggttgacac
     4321 aatactccac tccatggctc caacaagtag ttctagataa gcttggctgt tttggcggat
     4381 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
     4441 agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     4501 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc
     4561 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     4621 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
     4681 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt
     4741 aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt
     4801 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4861 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4921 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4981 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5041 gatccttgag agttttcgcc ccgaagaacg tgatgtccgg cggtgctttt gccgttacgc
     5101 accaccccgt cagtagctga acaggaggga cagctgatag aaacagaagc cactggagca
     5161 agcacctcaa aaacaccatc atacactaaa tcagtaagtt ggcagcatca cccgacgcac
     5221 tttgcgccga ataaatacct gtgacggaag atcacttcgc agaataaata aatcctggtg
     5281 tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac gttgatcggc
     5341 acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg
     5401 agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     5461 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     5521 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     5581 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     5641 ctcatccgga attacgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     5701 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     5761 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     5821 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     5881 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     5941 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     6001 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     6061 aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattgtcttc
     6121 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     6181 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     6241 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     6301 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     6361 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccgtactaa
     6421 ctgtcacgaa cccctgcaat aactgtcacg cccccctgca ataactgtca cgaacccctg
     6481 caataactgt cacgccccca aacctgcaaa cccagcaggg gcgggggctg gcggggtgtt
     6541 ggaaaaatcc atccatgatt atctaagaat aatccactag gcgcggttat cagcgccctt
     6601 gtggggcgct gctgcccttg cccaatatgc ccggccagag gccggatagc tggtctattc
     6661 gctgcgctag gctacacacc gccccaccgc tgcgcggcag ggggaaaggc gggcaaagcc
     6721 cgctaaaccc cacaccaaac cccgcagaaa tacgctggag cgcttttagc cgctttagcg
     6781 gcctttcccc ctacccgaag ggtgggggcg cgtgtgcagc cccgcagggc ctgtctcggt
     6841 cgatcattca gcccggctca tccttctggc gtggcggcag accgaacaag gcgcggtcgt
     6901 ggtcgcgttc aaggtacgca tccattgccg ccatgagccg atcctccggc cactcgctgc
     6961 tgttcacctt ggccaaaatc atggccccca ccagcacctt gcgccttgtt tcgttcttgc
     7021 gctcttgctg ctgttccctt gcccgctccc gctgaatttc ggcattgatt cgcgctcgtt
     7081 gttcttcgag cttggccagc cgatccgccg ccttgttgct ccccttaacc atcttgacac
     7141 cccattgtta atgtgctgtc tcgtaggcta tcatggaggc acagcggcgg caatcccgac
     7201 cctactttgt aggggagggc gcacttaccg gtttctcttc gagaaactgg cctaacggcc
     7261 acccttcggg cggtgcgctc tccgagggcc attgcatgga gccgaaaagc aaaagcaaca
     7321 gcgaggcagc atggcgattt atcaccttac ggcgaaaacc ggcagcaggt cgggcggcca
     7381 atcggccagg gccaaggccg actacatcca gcgcgaaggc aagtatgccc gcgacatgga
     7441 tgaagtcttg cacgccgaat ccgggcacat gccggagttc gtcgagcggc ccgccgacta
     7501 ctgggatgct gccgacctgt atgaacgcgc caatgggcgg ctgttcaagg aggtcgaatt
     7561 tgccctgccg gtcgagctga ccctcgacca gcagaaggcg ctggcgtccg agttcgccca
     7621 gcacctgacc ggtgccgagc gcctgccgta tacgctggcc atccatgccg gtggcggcga
     7681 gaacccgcac tgccacctga tgatctccga gcggatcaat gacggcatcg agcggcccgc
     7741 cgctcagtgg ttcaagcggt acaacggcaa gaccccggag aagggcgggg cacagaagac
     7801 cgaagcgctc aagcccaagg catggcttga gcagacccgc gaggcatggg ccgaccatgc
     7861 caaccgggca ttagagcggg ctggccacga cgcccgcatt gaccacagaa cacttgaggc
     7921 gcagggcatc gagcgcctgc ccggtgttca cctggggccg aacgtggtgg agatggaagg
     7981 ccggggcatc cgcaccgacc gggcagacgt ggccctgaac atcgacaccg ccaacgccca
     8041 gatcatcgac ttacaggaat accgggaggc aatagaccat gaacgcaatc gacagagtga
     8101 agaaatccag aggcatcaac gagttagcgg agcagatcga accgctggcc cagagcatgg
     8161 cgacactggc cgacgaagcc cggcaggtca tgagccagac caagcaggcc agcgaggcgc
     8221 aggcggcgga gtggctgaaa gcccagcgcc agacaggggc ggcatgggtg gagctggcca
     8281 aagagttgcg ggaggtagcc gccgaggtga gcagcgccgc gcagagcgcc cggagcgcgt
     8341 cgcgggggtg gcactggaag ctatggctaa ccgtgatgct ggcttccatg atgcctacgg
     8401 tggtgctgct gatcgcatcg ttgctcttgc tcgacctgac gccactgaca accgaggacg
     8461 gctcgatctg gctgcgcttg gtggcccgat gaagaacgac aggactttgc aggccatagg
     8521 ccgacagctc aaggccatgg gctgtgagcg cttcgatatc ggcgtcaggg acgcacccac
     8581 cggccagatg atgaaccggg aatggtcagc cgccgaagtg ctccagaaca cgccatggct
     8641 caagcggatg aatgcccagg gcaatgacgt gtatatcagg cccgccgagc aggagcggca
     8701 tggtctggtg ctggtggacg acctcagcga gtttgacctg gatgacatga aagccgaggg
     8761 ccgggagcct gccctggtag tggaaaccag cccgaagaac tatcaggcat gggtcaaggt
     8821 ggccgacgcc gcaggcggtg aacttcgggg gcagattgcc cggacgctgg ccagcgagta
     8881 cgacgccgac ccggccagcg ccgacagccg ccactatggc cgcttggcgg gcttcaccaa
     8941 ccgcaaggac aagcacacca cccgcgccgg ttatcagccg tgggtgctgc tgcgtgaatc
     9001 caagggcaag accgccaccg ctggcccggc gctggtgcag caggctggcc agcagatcga
     9061 gcaggcccag cggcagcagg agaaggcccg caggctggcc agcctcgaac tgcccgagcg
     9121 gcagcttagc cgccaccggc gcacggcgct ggacgagtac cgcagcgaga tggccgggct
     9181 ggtcaagcgc ttcggtcatg acctcagcaa gtgcgacttt atcgccgcgc agaagctggc
     9241 cagccggggc cgcagtgccg aggaaatcgg caaggccatg gccgaggcca gcccagcgct
     9301 ggcagagcgc aagcccggcc acgaagcgga ttacatcgag cgcaccgtca gcaaggtcat
     9361 gggtctgccc agcgtccagc ttgcgcgggc cgagctggca cgggcaccgg caccccgcca
     9421 gcgaggcatg gacaggggcg ggccagattt cagcatgtag tgcttgcgtt ggtactcacg
     9481 cctgttatac tatgagtact cacgcacaga agggggtttt atggaatacg aaaaaagcgc
     9541 ttcagggtcg gtctacctga tcaaaagtga caagggctat tggttgcccg gtggctttgg
     9601 ttatacgtca aacaaggccg aggctggccg cttttcagtc gctgatatgg ccagccttaa
     9661 ccttgacggc tgcaccttgt ccttgttccg cgaagacaag cctttcggcc ccggcaagtt
     9721 tctcggtgac tgatatgaaa gaccaaaagg acaagcagac cggcgacctg ctggccagcc
     9781 ctgacgctgt acgccaagcg cgatatgccg agcgcatgaa ggccaaaggg atgcgtcagc
     9841 gcaagttctg gctgaccgac gacgaatacg aggcgctgcg cgagtgcctg gaagaactca
     9901 gagcggcgca gggcgggggt agtgaccccg ccagcgccta accaccaact gcctgcaaag
     9961 gaggcaatca atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc
    10021 gccaccgctg gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc
    10081 gcccggtggt gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg
    10141 gccggatctg ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga
    10201 agacccgccc accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga
    10261 ggaacggcaa gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa
    10321 catcatggcc ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat
    10381 ggtgctggac acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc
    10441 ccaggtcatc ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct
    10501 gcaccatgcc agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg
    10561 gggcagctcg gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag
    10621 cgccgaggcc gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg
    10681 tgtgagcaag gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg
    10741 cggggtgctc aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg
    10801 tgaagcctaa gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg
    10861 cccccggcct gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga
    10921 cgtatgacta cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg
    10981 atgatctgcg catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg
    11041 gcccggaacc caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg
    11101 aggccgtcac cgctgaatgc catgtggtca aaggtagcta tcgggcgctg gcaaaggaaa
    11161 tcggggcaga ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc
    11221 tttggaaggt atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt
    11281 cggagtacgc cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg
    11341 cgcaggccgt catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc
    11401 tggacagcga aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca
    11461 aaaccggcaa ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg
    11521 gttcgaccat gcgcaagcgc cgcaagcggg tgcgcgaggc gttgccggag ctggtcgcgc
    11581 tgggctggac ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg
    11641 caggctgacc ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc
    11701 ttattttcct gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa
    11761 tattttgaaa aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct
    11821 ttgcttccag atgtatgctc ttctgctccc gaacgccagc aagacgtagc ccagcgcgtc
    11881 ggccagcttg caattcgcgc taacttacat taattgcgtt gcgctcactg cccgctttcc
    11941 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    12001 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga
    12061 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgtg gtttgcccca
    12121 gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg
    12181 tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg
    12241 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
    12301 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
    12361 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
    12421 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
    12481 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
    12541 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
    12601 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
    12661 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
    12721 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
    12781 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
    12841 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
    12901 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
    12961 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
    13021 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac cattcgatgg
    13081 tgtcaacgta aatgccgctt cgccttcgcg cgcgaattgc aagctgatcc gggcttatcg
    13141 actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg
    13201 tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa
    13261 tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctg
//
