LOCUS       sequence-100329-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule fused to dockerin type III 
            domain to perform single molecule force spectroscopy experiments; 
            covalently immobilized via introduced Cystein in Xylanase T120C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Schoeler C, Malinowska KH, Bernardi RC, Milles LF, Jobst MA, Durner 
            E, Ott W, Fried DB, Bayer EA, Schulten K, Gaub HE, Nash MA
  TITLE     Ultrastable cellulosome-adhesion complex tightens under load.
  JOURNAL   Nat Commun. 2014 Dec 8;5:5635. doi: 10.1038/ncomms6635.
  PUBMED    25482395
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-12-8"; volume: "5:5635. doi"; pages: " 
            10.1038/ncomms6635"
COMMENT     Sequence Label: pET28a-HIS-Xyl-Xmod-DocIII
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgagccacc atcaccatca ccataagaat
       61 gcagattcct atgcgaaaaa acctcacatc agcgcattga atgccccaca attggatcaa
      121 cgctacaaaa acgagttcac gattggtgcg gcagtagaac cttatcaact acaaaatgaa
      181 aaagacgtac aaatgctaaa gcgccacttc aacagcattg ttgccgagaa cgtaatgaaa
      241 ccgatcagca ttcaacctga ggaaggaaaa ttcaattttg aacaagcgga tcgaattgtg
      301 aagttcgcta aggcaaatgg catggatatt cgcttccata cactcgtttg gcacagccaa
      361 gtacctcaat ggttctttct tgacaaggaa ggtaagccaa tggttaatga atgcgatcca
      421 gtgaaacgtg aacaaaataa acaactgctg ttaaaacgac ttgaaactca tattaaaacg
      481 atcgtcgagc ggtacaaaga tgacattaag tactgggacg ttgtaaatga ggttgtgggg
      541 gacgacggaa aactgcgcaa ctctccatgg tatcaaatcg ccggcatcga ttatattaaa
      601 gtggcattcc aagcagctag aaaatatggc ggagacaaca ttaagcttta catgaatgat
      661 tacaatacag aagtcgaacc gaagcgaacc gctctttaca atttagtcaa acaactgaaa
      721 gaagagggtg ttccgatcga cggcatcggc catcaatccc acatcc
//
