LOCUS       sequence-10033-e       10982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10982)
  AUTHORS   Charpentier X, Gabay JE, Reyes M, Zhu JW, Weiss A, Shuman HA
  TITLE     Chemical genetics reveals bacterial and host cell functions critical
            for type IV effector translocation by Legionella pneumophila.
  JOURNAL   PLoS Pathog. 2009 Jul . 5(7):e1000501.
  PUBMED    19578436
REFERENCE   2  (bases 1 to 10982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Jul . 5(7):e1000501."
COMMENT     Sequence Label: pXDC61-RalF
FEATURES             Location/Qualifiers
     source          1..10982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..22,23..29)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    37..53
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     42..64
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             101..886
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     primer_bind     complement(247..266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(2066..2083)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2236..2322
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2414..2441
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2460..2551
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3002..3104
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3105..3764
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3171..3190)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      4096..4490
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            4832..4919
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             6158..7129
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDAPTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGHDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             7640..8479
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             8466..9317
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRKRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     10637..10656
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10676..10753)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca
       61 caggaaacag aattcagaag gagatataca tatggctcac ccagaaacgc tggtgaaagt
      121 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      181 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      241 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      301 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      361 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      421 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      481 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      541 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
      601 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      661 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      721 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      781 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
      841 aaatagacag atcgctgaga taggtgcctc actgattaag cattggggaa gcggtaccat
      901 gcatccagaa attgaaaaag cccaaagaga gattattgag gcattcaatg ccaagccaaa
      961 aaatgggata aataaaataa aagaaatatg tgaacaatat aaaatttctc ccaatgaaga
     1021 gatagctgag ttctttcatc aacaaagaaa aaatttggat ttagaagcgg ttggcgatta
     1081 tttaagcagc ccagaggcag aaaaccagca agtattaaaa gcttttacca gtcaaatgaa
     1141 tttcaatggg caaagttttg ttgagggttt aaggaccttt ttgaaaacat ttaaattacc
     1201 aggcgaagct caaaaaattg accggttagt gcaaagcttt agtggtgcct atttccagca
     1261 aaatcctgat gtggtttcca atgcggatgc tgcctatctt ttagcatttc aaaccattat
     1321 gcttaatact gatttacata acccaagtat ccctgagaaa aataaaatga ccgtggatgg
     1381 actcaaaaga aatctcagag gaggtaataa tggtggagat tttgatgcca aatttttaga
     1441 ggaactatac agtgagatta aagccaaacc ttttgagttg aatttcgtta aaaccagccc
     1501 aggatatgaa cttacttcaa ccactcttaa taaagattct acctttaaaa agctggattc
     1561 attcttgcat tcaacggatg taaacatcaa tacagttttt ccaggaatag gtgataatgt
     1621 taaaacaact gtagatcaac caaaatcatg gttaagtttt tttaccggat ataagggaac
     1681 aataacgttg acagataaca aaacatccgc acaagctact atccaggttt atactcccaa
     1741 tatattttca aaatggctat ttggagaaca accgagagta ataatacaac ctggacaaac
     1801 caaagagtca atcgatttgg cagcaaaagc agcagctgac ttttcatcgc cagtaaaaaa
     1861 ttttaaagcg acgtatgatt atgaggtagg tgatttaata aaagcatatg ataaccagaa
     1921 aaaactcatt acgattgaga gaaacctggc acttaaggag ggcgttccca aagatccaga
     1981 cgctgaaatg caaaaagaaa aaggtagaca attaaaattt tctagataag cttggctgtt
     2041 ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc
     2101 tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
     2161 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
     2221 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     2281 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     2341 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     2401 catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
     2461 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2521 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     2581 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     2641 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     2701 cagcggtaag atccttgaga gttttcgccc cgaagaacgt gatgtccggc ggtgcttttg
     2761 ccgttacgca ccaccccgtc agtagctgaa caggagggac agctgataga aacagaagcc
     2821 actggagcaa gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac
     2881 ccgacgcact ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa
     2941 atcctggtgt ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg
     3001 ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     3061 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     3121 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     3181 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     3241 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     3301 tgatgaatgc tcatccggaa ttacgtatgg caatgaaaga cggtgagctg gtgatatggg
     3361 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     3421 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     3481 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     3541 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     3601 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     3661 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     3721 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt
     3781 attgtcttca aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag
     3841 cgcagcgaat gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg
     3901 gtggctctgg taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg
     3961 catgttccgc gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc
     4021 ttttaccctc agccgaaatg cctgccgttg ctagacattg ccagccagtg cccgtcactc
     4081 ccgtactaac tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa taactgtcac
     4141 gaacccctgc aataactgtc acgcccccaa acctgcaaac ccagcagggg cgggggctgg
     4201 cggggtgttg gaaaaatcca tccatgatta tctaagaata atccactagg cgcggttatc
     4261 agcgcccttg tggggcgctg ctgcccttgc ccaatatgcc cggccagagg ccggatagct
     4321 ggtctattcg ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg gggaaaggcg
     4381 ggcaaagccc gctaaacccc acaccaaacc ccgcagaaat acgctggagc gcttttagcc
     4441 gctttagcgg cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc
     4501 tgtctcggtc gatcattcag cccggctcat ccttctggcg tggcggcaga ccgaacaagg
     4561 cgcggtcgtg gtcgcgttca aggtacgcat ccattgccgc catgagccga tcctccggcc
     4621 actcgctgct gttcaccttg gccaaaatca tggcccccac cagcaccttg cgccttgttt
     4681 cgttcttgcg ctcttgctgc tgttcccttg cccgctcccg ctgaatttcg gcattgattc
     4741 gcgctcgttg ttcttcgagc ttggccagcc gatccgccgc cttgttgctc cccttaacca
     4801 tcttgacacc ccattgttaa tgtgctgtct cgtaggctat catggaggca cagcggcggc
     4861 aatcccgacc ctactttgta ggggagggcg cacttaccgg tttctcttcg agaaactggc
     4921 ctaacggcca cccttcgggc ggtgcgctct ccgagggcca ttgcatggag ccgaaaagca
     4981 aaagcaacag cgaggcagca tggcgattta tcaccttacg gcgaaaaccg gcagcaggtc
     5041 gggcggccaa tcggccaggg ccaaggccga ctacatccag cgcgaaggca agtatgcccg
     5101 cgacatggat gaagtcttgc acgccgaatc cgggcacatg ccggagttcg tcgagcggcc
     5161 cgccgactac tgggatgctg ccgacctgta tgaacgcgcc aatgggcggc tgttcaagga
     5221 ggtcgaattt gccctgccgg tcgagctgac cctcgaccag cagaaggcgc tggcgtccga
     5281 gttcgcccag cacctgaccg gtgccgagcg cctgccgtat acgctggcca tccatgccgg
     5341 tggcggcgag aacccgcact gccacctgat gatctccgag cggatcaatg acggcatcga
     5401 gcggcccgcc gctcagtggt tcaagcggta caacggcaag accccggaga agggcggggc
     5461 acagaagacc gaagcgctca agcccaaggc atggcttgag cagacccgcg aggcatgggc
     5521 cgaccatgcc aaccgggcat tagagcgggc tggccacgac gcccgcattg accacagaac
     5581 acttgaggcg cagggcatcg agcgcctgcc cggtgttcac ctggggccga acgtggtgga
     5641 gatggaaggc cggggcatcc gcaccgaccg ggcagacgtg gccctgaaca tcgacaccgc
     5701 caacgcccag atcatcgact tacaggaata ccgggaggca atagaccatg aacgcaatcg
     5761 acagagtgaa gaaatccaga ggcatcaacg agttagcgga gcagatcgaa ccgctggccc
     5821 agagcatggc gacactggcc gacgaagccc ggcaggtcat gagccagacc aagcaggcca
     5881 gcgaggcgca ggcggcggag tggctgaaag cccagcgcca gacaggggcg gcatgggtgg
     5941 agctggccaa agagttgcgg gaggtagccg ccgaggtgag cagcgccgcg cagagcgccc
     6001 ggagcgcgtc gcgggggtgg cactggaagc tatggctaac cgtgatgctg gcttccatga
     6061 tgcctacggt ggtgctgctg atcgcatcgt tgctcttgct cgacctgacg ccactgacaa
     6121 ccgaggacgg ctcgatctgg ctgcgcttgg tggcccgatg aagaacgaca ggactttgca
     6181 ggccataggc cgacagctca aggccatggg ctgtgagcgc ttcgatatcg gcgtcaggga
     6241 cgcacccacc ggccagatga tgaaccggga atggtcagcc gccgaagtgc tccagaacac
     6301 gccatggctc aagcggatga atgcccaggg caatgacgtg tatatcaggc ccgccgagca
     6361 ggagcggcat ggtctggtgc tggtggacga cctcagcgag tttgacctgg atgacatgaa
     6421 agccgagggc cgggagcctg ccctggtagt ggaaaccagc ccgaagaact atcaggcatg
     6481 ggtcaaggtg gccgacgccg caggcggtga acttcggggg cagattgccc ggacgctggc
     6541 cagcgagtac gacgccgacc cggccagcgc cgacagccgc cactatggcc gcttggcggg
     6601 cttcaccaac cgcaaggaca agcacaccac ccgcgccggt tatcagccgt gggtgctgct
     6661 gcgtgaatcc aagggcaaga ccgccaccgc tggcccggcg ctggtgcagc aggctggcca
     6721 gcagatcgag caggcccagc ggcagcagga gaaggcccgc aggctggcca gcctcgaact
     6781 gcccgagcgg cagcttagcc gccaccggcg cacggcgctg gacgagtacc gcagcgagat
     6841 ggccgggctg gtcaagcgct tcggtcatga cctcagcaag tgcgacttta tcgccgcgca
     6901 gaagctggcc agccggggcc gcagtgccga ggaaatcggc aaggccatgg ccgaggccag
     6961 cccagcgctg gcagagcgca agcccggcca cgaagcggat tacatcgagc gcaccgtcag
     7021 caaggtcatg ggtctgccca gcgtccagct tgcgcgggcc gagctggcac gggcaccggc
     7081 accccgccag cgaggcatgg acaggggcgg gccagatttc agcatgtagt gcttgcgttg
     7141 gtactcacgc ctgttatact atgagtactc acgcacagaa gggggtttta tggaatacga
     7201 aaaaagcgct tcagggtcgg tctacctgat caaaagtgac aagggctatt ggttgcccgg
     7261 tggctttggt tatacgtcaa acaaggccga ggctggccgc ttttcagtcg ctgatatggc
     7321 cagccttaac cttgacggct gcaccttgtc cttgttccgc gaagacaagc ctttcggccc
     7381 cggcaagttt ctcggtgact gatatgaaag accaaaagga caagcagacc ggcgacctgc
     7441 tggccagccc tgacgctgta cgccaagcgc gatatgccga gcgcatgaag gccaaaggga
     7501 tgcgtcagcg caagttctgg ctgaccgacg acgaatacga ggcgctgcgc gagtgcctgg
     7561 aagaactcag agcggcgcag ggcgggggta gtgaccccgc cagcgcctaa ccaccaactg
     7621 cctgcaaagg aggcaatcaa tggctaccca taagcctatc aatattctgg aggcgttcgc
     7681 agcagcgccg ccaccgctgg actacgtttt gcccaacatg gtggccggta cggtcggggc
     7741 gctggtgtcg cccggtggtg ccggtaaatc catgctggcc ctgcaactgg ccgcacagat
     7801 tgcaggcggg ccggatctgc tggaggtggg cgaactgccc accggcccgg tgatctacct
     7861 gcccgccgaa gacccgccca ccgccattca tcaccgcctg cacgcccttg gggcgcacct
     7921 cagcgccgag gaacggcaag ccgtggctga cggcctgctg atccagccgc tgatcggcag
     7981 cctgcccaac atcatggccc cggagtggtt cgacggcctc aagcgcgccg ccgagggccg
     8041 ccgcctgatg gtgctggaca cgctgcgccg gttccacatc gaggaagaaa acgccagcgg
     8101 ccccatggcc caggtcatcg gtcgcatgga ggccatcgcc gccgataccg ggtgctctat
     8161 cgtgttcctg caccatgcca gcaagggcgc ggccatgatg ggcgcaggcg accagcagca
     8221 ggccagccgg ggcagctcgg tactggtcga taacatccgc tggcagtcct acctgtcgag
     8281 catgaccagc gccgaggccg aggaatgggg tgtggacgac gaccagcgcc ggttcttcgt
     8341 ccgcttcggt gtgagcaagg ccaactatgg cgcaccgttc gctgatcggt ggttcaggcg
     8401 gcatgacggc ggggtgctca agcccgccgt gctggagagg cagcgcaaga gcaagggggt
     8461 gccccgtggt gaagcctaag aacaagcaca gcctcagcca cgtccggcac gacccggcgc
     8521 actgtctggc ccccggcctg ttccgtgccc tcaagcgggg cgagcgcaag cgcagcaagc
     8581 tggacgtgac gtatgactac ggcgacggca agcggatcga gttcagcggc ccggagccgc
     8641 tgggcgctga tgatctgcgc atcctgcaag ggctggtggc catggctggg cctaatggcc
     8701 tagtgcttgg cccggaaccc aagaccgaag gcggacggca gctccggctg ttcctggaac
     8761 ccaagtggga ggccgtcacc gctgaatgcc atgtggtcaa aggtagctat cgggcgctgg
     8821 caaaggaaat cggggcagag gtcgatagtg gtggggcgct caagcacata caggactgca
     8881 tcgagcgcct ttggaaggta tccatcatcg cccagaatgg ccgcaagcgg caggggtttc
     8941 ggctgctgtc ggagtacgcc agcgacgagg cggacgggcg cctgtacgtg gccctgaacc
     9001 ccttgatcgc gcaggccgtc atgggtggcg gccagcatgt gcgcatcagc atggacgagg
     9061 tgcgggcgct ggacagcgaa accgcccgcc tgctgcacca gcggctgtgt ggctggatcg
     9121 accccggcaa aaccggcaag gcttccatag ataccttgtg cggctatgtc tggccgtcag
     9181 aggccagtgg ttcgaccatg cgcaagcgcc gcaagcgggt gcgcgaggcg ttgccggagc
     9241 tggtcgcgct gggctggacg gtaaccgagt tcgcggcggg caagtacgac atcacccggc
     9301 ccaaggcggc aggctgaccc cccccactct attgtaaaca agacattttt atcttttata
     9361 ttcaatggct tattttcctg ctaattggta ataccatgaa aaataccatg ctcagaaaag
     9421 gcttaacaat attttgaaaa attgcctact gagcgctgcc gcacagctcc ataggccgct
     9481 ttcctggctt tgcttccaga tgtatgctct tctgctcccg aacgccagca agacgtagcc
     9541 cagcgcgtcg gccagcttgc aattcgcgct aacttacatt aattgcgttg cgctcactgc
     9601 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     9661 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
     9721 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgtgg
     9781 tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc
     9841 tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact
     9901 cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg
     9961 gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt
    10021 cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag
    10081 ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt
    10141 gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa
    10201 tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg
    10261 cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga
    10321 cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta
    10381 ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa
    10441 tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt
    10501 tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt
    10561 ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg
    10621 tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca
    10681 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcacc
    10741 attcgatggt gtcaacgtaa atgccgcttc gccttcgcgc gcgaattgca agctgatccg
    10801 ggcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
    10861 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
    10921 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
    10981 tg
//
