LOCUS       sequence-100332-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule fused to cohesin type III domain
            to perform single molecule force spectroscopy experiments; 
            covalently immobilized via introduced ybbR tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Schoeler C, Malinowska KH, Bernardi RC, Milles LF, Jobst MA, Durner 
            E, Ott W, Fried DB, Bayer EA, Schulten K, Gaub HE, Nash MA
  TITLE     Ultrastable cellulosome-adhesion complex tightens under load.
  JOURNAL   Nat Commun. 2014 Dec 8;5:5635. doi: 10.1038/ncomms6635.
  PUBMED    25482395
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-12-8"; volume: "5:5635. doi"; pages: " 
            10.1038/ncomms6635"
COMMENT     Sequence Label: pET28a-CohIII-CBM-HIS-ybbR
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(58..81)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(88..105)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcagccgga tcttactcga gttaagccag tttagaagcg atgaattcca gagagtccgg
       61 accctggaac agaacttcca gggatccgtg gtggtggtgg tggtgcctag acctaggaag
      121 cttgagctca ccgggttctt taccccatac aagaacaccg ttcaagtatg ctgttacctg
      181 atcccattca acaaactgtg aagcagactt gaatgagtag tcatttgact gtgtatagtt
      241 actccagtca ttctttgcaa atctaccttg tatctgaaca tgtgcacccg gttcaagagt
      301 tccgcctgta aagcttattt caaggtaggt gtctgcgtta tttgttgagg aactcatttt
      361 tacaaatgtt ccttttacat ttgaagtaat tccgttgtag ctgccgttac tgccgattat
      421 tgcagcatgg tcactccaga aggtctgatc tttctgaccg tctactgtat aataatatct
      481 caatgtgagt ttggacaaat caattgcact gcttccggta ttagtaacct tgaactgagg
      541 attgattgag ttagtagtat ctgaaggatt gctgttgtag aattcaacct tcaaattgcc
      601 tgataccggt gtattggcca tggcattcgg atcatctgac ggcggtattg ttgttgcagg
      661 tggttttgtt gttgcaggtg gtgttgttac aggctgtgtt gatggtacta cactgcctgg
      721 ctcaccagcc ttgattgcaa tgtaaccatc agcatatgta gcgttag
//
