LOCUS       sequence-100335-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pENTR vector for reptilian orthoreovirus p14 fusion-associated small
            transmembrane (FAST) protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Cachat E, Liu W, Hohenstein P, Davies JA
  TITLE     A library of mammalian effector modules for synthetic morphology.
  JOURNAL   J Biol Eng. 2014 Nov 19;8(1):26. eCollection 2014.
  PUBMED    25478005
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-11-19"; volume: "8(1)"; pages: "26. eCollection 
            2014"
COMMENT     Sequence Label: pENTR-p14FAST
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(465..564)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(581..600)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(582..600)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(605..621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(605..621)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(723..742)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtacaa aaaagcaggc ttcaccatgg ggagtggacc ctctaatttc gtcaatcacg
      121 cacctggaga agcaattgta accggtttgg agaaaggggc agataaagta gctggaacga
      181 tatcacatac gatttgggaa gtgatcgccg gattagtagc cttgctgaca ttcttagcgt
      241 ttggcttctg gttgttcaag tatctccaaa agagaagaga aagaaggaga caactcactg
      301 agttccaaaa acggtatcta cggaatagct acaggttgag tgagatccag agacctatat
      361 cacagcacga atacgaagac ccatacgagc caccaagtcg taggaaacca ccccctcctc
      421 cttatagcac atacgtcaac atcgataatg tctcagccat ttagacccag ctttcttgta
      481 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
      541 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
      601 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
      661 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
      721 tacaaggggt gttatgagcc atattcaac
//
