LOCUS       sequence-100342-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterially expressed GST-tagged human polo-like kinase 1 PBD AA 
            365-653 H538A/K540M mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Yeh TY, Kowalska AK, Scipioni BR, Cheong FK, Zheng M, Derewenda U, 
            Derewenda ZS, Schroer TA
  TITLE     Dynactin helps target Polo-like kinase 1 to kinetochores via its 
            left-handed beta-helical p27 subunit.
  JOURNAL   EMBO J. 2013 Apr 3;32(7):1023-35. doi: 10.1038/emboj.2013.30. Epub 
            2013 Mar 1.
  PUBMED    23455152
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2013.30"; journalName: "EMBO J"; date: "2013-04-3- 
            3"; volume: "32"; issue: "7"; pages: "1023-35"
COMMENT     Sequence Label: GST-PBD H538A/K540M
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(916..933)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(960..982)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 aacgcgcgag gcagatcgtc agtcagtcac gatgcggccg ctcgagtcga ctggcgcgcc
       61 ttaggaggcc ttgagacggt tgctggccga gcgtgagctc agcagcttgt ccaccatagt
      121 gcgggcgtag cggagccggc tggccagctc cttgcagcag ccgtactcct ccaggagact
      181 caggcggtat gtgcggaagt cccgcttctc gtcgatgtag gtcacggctg ccatcagtgg
      241 gcacaagatg agcatggtgg catcctggaa gaagttgatc tgcacgctgc cgttgctgag
      301 gtgcaggatg atggcgctgc gggtgcggaa ccaggtccgt aggtagggca gccgagcgag
      361 ctcatcacct tcgcgcggcg tgatgttggc acctgccttc agcaagtgct cgctcatgta
      421 attgcggaaa tatttaagga gggtgatctt cttcatcaag gagttgggat gggaactcac
      481 ggtgaggtag gactcagtgc cgtcacgctc tatgtactgc aggctgtcac catcattgta
      541 gaggatgagg cgtgttgagt cattgaagag cacccccacg ctgttatcac agagctgata
      601 cccaaggccg tacttgtccg aatagtccac ccacttgctg acccagaaga tggggatgca
      661 ggcaggatcc tcagcctcct cttgcctgac cagcccacgc tccgagggct tggaggcatt
      721 gacactgtgc agctgctgca gcatgtcact gaggtggcag tcgaccacct cacctgtctc
      781 tcgaaccact ggttcttctt tttcccgggg acgctcaggc agggggttct ccaagccttt
      841 attgaggact gtgaggggct tccggttgct ggggtccagg ctgctgggag caatcgatgg
      901 ccggccgaat tccggggatc cacgcggaac cagatccgat tttggaggat ggtcgccacc
      961 accaaacgtg gcttgccagc cctgcaaagg ccatgctata tacttgctgg atttcaagta
     1021 cttatcattt gtgggatagc ttcatacgtt ttttaaacaa actaattttg gga
//
