LOCUS       sequence-100359-       12740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombinase-mediated cassette exchange in ES cells to insert a Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 12740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: Ai62(TITL-tdT) Flp-in replacement vector
FEATURES             Location/Qualifiers
     source          1..12740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    414..447
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     promoter        2993..3307
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    3001..3019
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3037..3055
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3072..3090
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3108..3126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3144..3162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3179..3197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3215..3233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3280..3304
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(3317..3350)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3428..3476
                     /note="synthetic polyadenylation signal"
     polyA_signal    3485..3961
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3684..3703)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     polyA_signal    3973..4434
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    complement(4461..4494)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4521..4530
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4660..4678)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4928..4947
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5122..5145
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    5982..6570
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6035..6055)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6453..6464)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6595..6612)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6601..6808
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    9312..9345
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        9372..9871
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9407..9430)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9804..9823
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(10519..10535)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10519..10535)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10532..10554)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10543..10559
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10567..10573,10574..10591,10592..10597))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10612..10633
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10750..10767)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10921..11509)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11001..11020)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11680..12471,12472..12540))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12303..12322
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12541..12645)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12713..12731
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc
      421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattgga tccgttttat
      481 gccgcggtta ccgtgaagat atcttaatta agaattcgag ctcgctagag ccccatcctc
      541 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt
      601 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc
      661 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac
      721 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag
      781 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc
      841 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc
      901 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt
      961 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat
     1021 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa
     1081 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc
     1141 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc
     1201 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat
     1261 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg
     1321 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc
     1381 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc
     1441 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa
     1501 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     1561 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     1621 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     1681 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt cccgagctcg
     1741 gtaccgcccc atcctcactg actccgtcct ggagttggat gagagataat ggccttacgt
     1801 tgtgccaggg gagggtcggg ctggatttag caagatttac cttctccaaa gagcggtgct
     1861 gcagtggcac agctgcccac ggaggtgggg gggtcaccgt ccctggaggt gatgaagaac
     1921 tgtggggatg tggcactgag ggacatggcc agtgggcacg gtgggtgggt tggggttggt
     1981 cttggggatc ttggagggct tttccagcct tcatgatttg acgattgtat gaacatctac
     2041 atggcaattc tccagctgcc tgtcccagtc ctactgaccc agctgtatct ctccaggcaa
     2101 gctcttccac cccttctgct tgcatccaga caccatcaaa catgcaggct cagacacagg
     2161 gaccagcagt gtctgtggcc tttttgtgct cctctccatg ctgggtttta acttgctctt
     2221 tgtccttcta tcctatcttc ttatccttaa ggctgttctg aacgctgtga cttggagagt
     2281 gtcccagagc cctcaacacc tgcatgtccc acgtccatgc tgtcctgcac ttccttatcc
     2341 ccaagatctg cctctccgtg atgcactgaa ttggcaaaca tgtgtcaccc cagaccaaca
     2401 atgtcacagc aaactccccc ttgataggac aagggggaat ggctttacac tgagacaggg
     2461 gaggtttggg ttggatatga ggaggcagtt tttcccccag agggtggtga cgcactgaac
     2521 aggttgccca aggaggctgt ggatgcccca tccctgcagg cattcaaggc caggctggat
     2581 gtggctctgg gcagcctggg ctgctggttg atgaccctgc acatagcagg gggttggatc
     2641 tggatgagca ctgtgctcct ttgcaaccca ggccgttcta tgattctgtc attctaaatc
     2701 tctctttcag cctaaagctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc
     2761 agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc
     2821 cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg
     2881 ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc tttggggggg
     2941 ggctgtcccg gtacccgggg atccaccggt atcgattacc tatcacaaat tcgagtttac
     3001 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat
     3061 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     3121 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc
     3181 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
     3241 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
     3301 gatcgcctat ttaaatataa cttcgtatag catacattat acgaagttat tcgcgagtga
     3361 ataaatgaaa gcttgcagat cccctcagca ccatggctag cggcagcctc ggagtttgaa
     3421 tagatagaat aaaatatctt tattttcatt ccatctgtgt gttggttttt tgtgtgagat
     3481 ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac
     3541 tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt
     3601 gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa
     3661 gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc
     3721 ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc aggctcccga
     3781 gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag
     3841 acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc
     3901 accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct
     3961 tctgcctcag cgctgtaagt ctgcagaaat tgatgatcta ttaaacaata aagatgtcca
     4021 ctaaaatgga agtttttcct gtcatacttt gttaagaagg gtgagaacag agtacctaca
     4081 ttttgaatgg aaggattgga gctacggggg tgggggtggg gtgggattag ataaatgcct
     4141 gctctttact gaaggctctt tactattgct ttatgataat gtttcatagt tggatatcat
     4201 aatttaaaca agcaaaacca aattaagggc cagctcattc ctcccactca tgatctatag
     4261 atctatagat ctctcgtggg atcattgttt ttctcttgat tcccactttg tggttctaag
     4321 tactgtggtt tccaaatgtg tcagtttcat agcctgaaga acgagatcag cagcctctgt
     4381 tccacataca cttcattctc agtattgttt tgccaagttc taattccatc agaaagcttt
     4441 taattaactg aatagctaac ataacttcgt atagcataca ttatacgaag ttatcacgcg
     4501 tgtttgggac gcgtgctagc gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt
     4561 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
     4621 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     4681 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
     4741 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
     4801 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
     4861 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
     4921 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
     4981 acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg
     5041 gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg
     5101 gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg
     5161 tggaacagta cgagcgctcc gagggccgcc accacctgtt cctggggcat ggtaccggca
     5221 gcaccggcag cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtgatta
     5281 aggagtttat gaggtttaaa gtcaggatgg agggcagcat gaacgggcac gagtttgaga
     5341 ttgaaggaga gggggaaggg aggccctatg agggcaccca gacagcaaag ctgaaagtga
     5401 caaagggcgg ccctctgcca ttcgcatggg acatcctgtc tcctcagttt atgtatggaa
     5461 gtaaagccta cgtgaaacac cctgctgaca ttccagatta caagaaactg agcttcccag
     5521 aggggtttaa gtgggaaaga gtgatgaact tcgaagacgg gggcctggtg actgtcaccc
     5581 aggacagctc tctgcaggac ggcactctga tctataaggt gaaaatgcgc ggcaccaatt
     5641 ttccccctga tggcccagtc atgcagaaga agacaatggg ctgggaggct tcaacagaaa
     5701 ggctgtaccc cagagacggc gtgctgaagg gagagatcca ccaggcactg aagctgaaag
     5761 atggagggca ttatctggtc gaattcaaaa ccatctacat ggccaagaaa cccgtgcagc
     5821 tgcctggcta ctattacgtc gacacaaagc tggatattac tagccataac gaagactaca
     5881 ccattgtgga gcagtatgag cggagcgagg ggaggcacca tctgtttctg tacggcatgg
     5941 acgagctgta caagtaaacg cgttacgtaa agcttgtcga taatcaacct ctggattaca
     6001 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6061 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6121 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6181 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6241 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6301 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6361 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctatgtt gccacctgga
     6421 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6481 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     6541 gtcggatctc cctttgggcc gcctccccgc atcgacaccg agcgctgctc gaggcctcga
     6601 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     6661 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     6721 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     6781 gggaagacaa tagcaggcat gctggggaca cgtgggatcc tctagtctag agccccatcc
     6841 tcactgactc cgtcctggag ttggatgaga gataatggcc ttacgttgtg ccaggggagg
     6901 gtcgggctgg atttagcaag atttaccttc tccaaagagc ggtgctgcag tggcacagct
     6961 gcccacggag gtgggggggt caccgtccct ggaggtgatg aagaactgtg gggatgtggc
     7021 actgagggac atggccagtg ggcacggtgg gtgggttggg gttggtcttg gggatcttgg
     7081 agggcttttc cagccttcat gatttgacga ttgtatgaac atctacatgg caattctcca
     7141 gctgcctgtc ccagtcctac tgacccagct gtatctctcc aggcaagctc ttccacccct
     7201 tctgcttgca tccagacacc atcaaacatg caggctcaga cacagggacc agcagtgtct
     7261 gtggcctttt tgtgctcctc tccatgctgg gttttaactt gctctttgtc cttctatcct
     7321 atcttcttat ccttaaggct gttctgaacg ctgtgacttg gagagtgtcc cagagccctc
     7381 aacacctgca tgtcccacgt ccatgctgtc ctgcacttcc ttatccccaa gatctgcctc
     7441 tccgtgatgc actgaattgg caaacatgtg tcaccccaga ccaacaatgt cacagcaaac
     7501 tcccccttga taggacaagg gggaatggct ttacactgag acaggggagg tttgggttgg
     7561 atatgaggag gcagtttttc ccccagaggg tggtgacgca ctgaacaggt tgcccaagga
     7621 ggctgtggat gccccatccc tgcaggcatt caaggccagg ctggatgtgg ctctgggcag
     7681 cctgggctgc tggttgatga ccctgcacat agcagggggt tggatctgga tgagcactgt
     7741 gctcctttgc aacccaggcc gttctatgat tctgtcattc taaatctctc tttcagccta
     7801 aagctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg agaagcgttc
     7861 agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca cgctgccggc
     7921 tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg ctgctgcccc
     7981 ctagcggggg agggacgtaa ttacatccct gggggctttg ggggggggct gtccctctag
     8041 agccccatcc tcactgactc cgtcctggag ttggatgaga gataatggcc ttacgttgtg
     8101 ccaggggagg gtcgggctgg atttagcaag atttaccttc tccaaagagc ggtgctgcag
     8161 tggcacagct gcccacggag gtgggggggt caccgtccct ggaggtgatg aagaactgtg
     8221 gggatgtggc actgagggac atggccagtg ggcacggtgg gtgggttggg gttggtcttg
     8281 gggatcttgg agggcttttc cagccttcat gatttgacga ttgtatgaac atctacatgg
     8341 caattctcca gctgcctgtc ccagtcctac tgacccagct gtatctctcc aggcaagctc
     8401 ttccacccct tctgcttgca tccagacacc atcaaacatg caggctcaga cacagggacc
     8461 agcagtgtct gtggcctttt tgtgctcctc tccatgctgg gttttaactt gctctttgtc
     8521 cttctatcct atcttcttat ccttaaggct gttctgaacg ctgtgacttg gagagtgtcc
     8581 cagagccctc aacacctgca tgtcccacgt ccatgctgtc ctgcacttcc ttatccccaa
     8641 gatctgcctc tccgtgatgc actgaattgg caaacatgtg tcaccccaga ccaacaatgt
     8701 cacagcaaac tcccccttga taggacaagg gggaatggct ttacactgag acaggggagg
     8761 tttgggttgg atatgaggag gcagtttttc ccccagaggg tggtgacgca ctgaacaggt
     8821 tgcccaagga ggctgtggat gccccatccc tgcaggcatt caaggccagg ctggatgtgg
     8881 ctctgggcag cctgggctgc tggttgatga ccctgcacat agcagggggt tggatctgga
     8941 tgagcactgt gctcctttgc aacccaggcc gttctatgat tctgtcattc taaatctctc
     9001 tttcagccta aagctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg
     9061 agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca
     9121 cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg
     9181 ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg ggggggggct
     9241 gtccctctag aagtcgacgg cgcgccgtaa aagcttggca tagatcttat tgcagaagcc
     9301 atatgtttta ggtgccaggg cgtgcccttg ggctccccgg gcgcgtttgg gcggccgcat
     9361 cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     9421 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     9481 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     9541 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     9601 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     9661 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     9721 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     9781 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     9841 gttctcctct tcctcatctc cgggcctttc gacctgcaat cgccgctagc cgccaccatg
     9901 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     9961 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
    10021 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
    10081 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
    10141 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
    10201 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
    10261 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
    10321 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
    10381 tggcaaactg tgatggacga caccgtcagt gtaagtgcta gcccctaggc ctgaatcgaa
    10441 gttcctattc ttcaaaaggt ataggaactt cgagctcgtc gactgcagag gcctgcatgc
    10501 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    10561 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    10621 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    10681 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    10741 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10801 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10861 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10921 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10981 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    11041 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    11101 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    11161 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    11221 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    11281 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    11341 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    11401 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    11461 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    11521 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    11581 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    11641 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    11701 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    11761 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    11821 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    11881 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    11941 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    12001 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    12061 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    12121 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    12181 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    12241 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    12301 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    12361 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    12421 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    12481 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    12541 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    12601 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    12661 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    12721 tatcacgagg ccctttcgtc
//
