LOCUS       sequence-100361-       16291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target a Dre and Cre-dependent tdTomato expression cassette to the 
            mouse Rosa26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16291)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 16291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: Ai66(RCRL-tdT) targeting vector
FEATURES             Location/Qualifiers
     source          1..16291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..1085
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     enhancer        1181..1484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1486..1763
                     /label=chicken beta-actin promoter
     intron          1765..2782
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2706..2728
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2790..2809
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2864..2895
                     /label=rox
                     /bound_moiety="Dre recombinase"
     polyA_signal    2941..2989
                     /note="synthetic polyadenylation signal"
     polyA_signal    2998..3474
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3197..3216)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3509..3528)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3553..3601
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    3761..3792
                     /label=rox
                     /bound_moiety="Dre recombinase"
     protein_bind    3807..3840
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4007..4141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4261..4395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4515..4649
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    4678..4711
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4800..4809
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4806..6236
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    6315..6903
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6368..6388)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6786..6797)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6906..6922)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6907..6923)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6924..6941)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6930..7137
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7145..7178
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        7200..7699
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7235..7258)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7632..7651
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        7716..7763
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7791..8594
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7854..7873)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8464..8483
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8604..9065
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     misc_feature    9129..13398
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(13443..13463)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13443..13461)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13482..13498)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13482..13498)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13495..13517)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13506..13522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13530..13536,13537..13554,13555..13560))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13575..13596
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13713..13730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13884..14472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13964..13983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14643..15434,15435..15503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15266..15285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15504..15608)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(15634..16089)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15771..15792)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15983..16002
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16216..16238
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     16230..16247
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16231..16247
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16257..16276
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16257..16275
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggascgctt ccggccgacg tctcgtcgct gattggctty ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgn acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg
      421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttccccc tcttccctcg tgatctgcaa ctccagtctt
     1081 tctagcctta attaaccgtt taaacaattc tgcaggaatc tagttattaa tagtaatcaa
     1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     1501 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     1561 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
     1621 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
     1681 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     1741 aaaagcgaag cgcgcggcgg gcggggagtc gctgcgacgc tgccttcgcc ccgtgccccg
     1801 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     1861 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
     1921 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg
     1981 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc
     2041 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag
     2101 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg
     2161 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc
     2221 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc
     2281 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg
     2341 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg
     2401 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     2461 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
     2521 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
     2581 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     2641 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
     2701 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     2761 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     2821 ttttggcaaa gaattgattt gataccgcgg gcccgtattc gaataacttt aaataatgcc
     2881 aattatttaa agttacctca gcaccatggc tagcggcagc ctcggagttt gaatagatag
     2941 aataaaatat ctttattttc attccatctg tgtgttggtt ttttgtgtga gatctacggg
     3001 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     3061 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     3121 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     3181 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     3241 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcaggctcc cgagttgttg
     3301 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     3361 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     3421 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgcct
     3481 cagctaattg agtagggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     3541 cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt
     3601 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     3661 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     3721 ggcccagggc tcgcagccaa cgtcggggcg gcaggccgtg taactttaaa taatgccaat
     3781 tatttaaagt tactcgagat ttaaatataa cttcgtataa tgtatgctat acgaagttat
     3841 tcgcgatgaa taaatgaaag cttgcagatc tgcgactcta gaggatctgc gactctagag
     3901 gatcataatc agccntacca catttgtaga ggttttactn gctttaaaaa acctcccaca
     3961 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     4021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     4141 ctgcgactct agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     4201 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     4261 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4321 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4381 tatcatgtct ggatctgcga ctctagagga tcataatcag ccataccaca tttgtagagg
     4441 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     4501 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4561 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4621 tcatcaatgt atcttatcat gtctggatcc ccatcaagct gatccggaac ccttaatata
     4681 acttcgtata atgtatgcta tacgaagtta ttaggtccct cgacctgcag cccaagctag
     4741 atcgaattcg gccggccttc acgtgttgca cttaacgcgt ggccggcctt cacgatcccg
     4801 ccaccatggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca
     4861 tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     4921 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     4981 gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg
     5041 acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     5101 acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc
     5161 tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga
     5221 agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
     5281 agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
     5341 agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca
     5401 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg
     5461 agggccgcca ccacctgttc ctggggcatg gcaccggcag caccggcagc ggcagctccg
     5521 gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg
     5581 tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     5641 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     5701 tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc
     5761 ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
     5821 tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg
     5881 gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa
     5941 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg
     6001 tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg
     6061 agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg
     6121 acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
     6181 gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac aagtaagaat
     6241 tgtgttgcac ttaacgcgtt aagtgcaaca cgtgaaggcc ggccctgcag gaattcgata
     6301 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6361 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6421 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6481 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6541 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6601 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6661 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6721 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6781 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6841 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6901 cgcatcgata ccgtcgacct cgacctcgac tgtgccttct agttgccagc catctgttgt
     6961 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     7021 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     7081 ggtggggcag gacagcaagg gggaggattg ggaagacaat ggcaggcatg ctggggaact
     7141 agtggtgcca gggcgtgccc ttgggctccc cgggcgcggc ggccgcatcg aattctaccg
     7201 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     7261 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     7321 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     7381 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     7441 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     7501 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     7561 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     7621 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     7681 ctcatctccg ggcctttcga cctgcagcag cacgtgttga caattaatca tcggcatagt
     7741 atatcggcat agtataatac gacaaggtga ggaactaaac cgctagcacc atgggatcgg
     7801 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     7861 gctatgactg ggcacaacag acgatcggct gctctgatgc cgccgtgttc cggctgtcag
     7921 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     7981 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     8041 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     8101 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     8161 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     8221 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     8281 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     8341 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     8401 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     8461 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     8521 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     8581 acgagttctt ctgaggggat ccgctgtaag tctgcagaaa ttgatgatct attaaacaat
     8641 aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag ggtgagaaca
     8701 gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg ggtgggatta
     8761 gataaatgcc tgctctttac tgaaggctct ttactattgc tttatgataa tgtttcatag
     8821 ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc
     8881 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt
     8941 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca
     9001 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat
     9061 cagaaagctt gcagaagatc tccccaactg gggtaacctt tgagttctct cagttggggg
     9121 ggcgcgccct agaagatggg cgggagtctt ctgggcaggc ttaaaggcta acctggtgtg
     9181 tgggcgttgt cctgcagggg aattgaacag gtgtaaaatt ggagggacaa gacttcccac
     9241 agattttcgg ttttgtcggg aagtttttta ataggggcaa ataggaaaat ggaggatagg
     9301 agtcatctgg ggtttatgca gcaaaactac aggtatattg cttgtatccg cctcggagat
     9361 ttccatgagg agataaagac atgtcacccg agtttatact ctcctgctta gatcctacta
     9421 cagtatgaaa tacagtgtyg cgaggtagac tatgtaagca gatttaatca ttttaaagag
     9481 cccagtactt catatccatt tctcccgctc cttctgcagc cttatcaaaa ggtatttaga
     9541 acactcattt tagccccatt ttcatttatt atactggctt atccaacccc tagacagagc
     9601 attggcattt tccctttcct gatcttagaa gtctgatgac tcatgaaacc agacagatta
     9661 gttacataca ccacaaatcg aggctgtagc tggggcctca acactgcagt tcttttataa
     9721 ctccttagta cactttttgt tgatcctttg ccttgatcct taattttcag tgtctatcac
     9781 ctctcccgtc aggtggtgtt ccacatttgg gcctattctc agtccaggga gttttacaac
     9841 aatagatgta ttgagaatcc aacctaaagc ttaactttcc actcccatga atgcctctct
     9901 cctttttctc cattataact gagctatwac cattaatggt ttcaggtgga tgtctcctcc
     9961 cccaatatac ctgatgtatc tacatattgc caggctgata ttttaagaca twaaaggtat
    10021 atttcattat tgagccacat ggtattgatt actgctacta aaattttgtc attgtacaca
    10081 tctgtaaaag gtggttcctt ttggaatgca aagttcaggt gtttgttgtc tttcctgacc
    10141 taaggtcttg tgagcttgta ttttttctat ttaagcagtg ctttctcttg gactggcttg
    10201 actcatggca ttctacacgt tattgctggt ctaaatgtga ttttgccaag cttcttcagg
    10261 acctataatt ttgcttgact tgtagccaaa cacaagtaaa atgattaagc aacaaatgta
    10321 tttgtgaagc ttggttttta ggttgttgtg ttgtgtgtgc ttgtgctcta taataatact
    10381 atccaggggc tggagaggtg gctcggagtt caagagcaca gactgctctt ccagaagtcc
    10441 tgagttcaat tcccagcaac cacatggtgg ctcacaacca tctgtaatgg gatctgatgc
    10501 cctcttctgg tgtgtctgaa gaccacaagt gtattcacat taaataaata atcctccttc
    10561 ttcttctttt ttttttttta aagagaatwc tgtctccagt agaattactg aagtaatgaa
    10621 atactttgtg tttgttccaa tatggwagcc aataatcaaa tactcttwag cactggaaat
    10681 gtaccaagga actattttat ttaagtgwac tgtggacaga ggagccataa ctgcagactt
    10741 gtgggataca gaagaccaat gcagacttaa tgtcttttct cttacactaa gcaataaaga
    10801 aataaaaatt gaacttctag tatcctattt gttaaactgc tagctttact aacttttgtg
    10861 cttcatctat acaaagctga aagctaagtc tgcagccatt actaaacatg aaagcaagta
    10921 atgataattt tggatttcaa aaatgtaggg ccagagttta gccagccagt ggtggtgctt
    10981 gcctttatgc cttaatccca gcactctgga ggcagagaca ggcagatctc tgagtttgag
    11041 cccagcctgg tctacacatc aagttctatc taggatagcc aggaatacac acagaaaccc
    11101 tgttggggag gggggctctg agatttcata aaattataat tgaagcattc cctaatgagc
    11161 cactatggat gtggctaaat ccgtctacct ttctgatgag atttgggtat tattttttct
    11221 gtctctgctg ttggttgggt cttttgacac tgtgggcttt cttaaagcct ccttccctgc
    11281 catgtggtct cttgtttgct actaacttcc catggcttaa atggcatggc tttttgcctt
    11341 ctaagggcag ctgctgagwt ttgcagcctg atttccaggg tggggttggg aaatctttca
    11401 aacactaaaa ttgtccttta attttttttt aaaaaatggg ttatataata aacctcataa
    11461 aatagttatg aggagtgagg tggactaata ttaatgagtc cctcccctat aaaagagcta
    11521 ttaaggcttt ttgtcttata ctaacttttt ttttaaatgt ggtatcttta gaaccaaggg
    11581 tcttagagtt ttagtataca gaaactgttg catcgcttaa tcagattttc tagtttcaaa
    11641 tccagagaat ccaaattctt cacagccaaa gtcaaattaa gaatttctga ctttaatgtt
    11701 atttgctact gtgaatataa aatgatagct tttcctgagg cagggtctca ctatgtatct
    11761 ctgcctgatc tgcaacaaga tatgtagact aaagttctgc ctgcttttgt ctcctgaata
    11821 ctaaggttaa aatgtagtaa tacttttgga acttgcaggt cagattcttt tataggggac
    11881 acactaaggg agcttgggtg atagttggta aatgtgttta agtgatgaaa acttgaatta
    11941 ttatcaccgc aacctacttt ttaaaaaaaa aagccaggcc tgttagagca tgctaaggga
    12001 tccctaggac ttgctgagca cacaagagta gtacttggca ggctcctggt gagagcatat
    12061 ttcaaaaaac aaggcagaca accaagaaac tacagtaagg ttacctgtct ttaaccatct
    12121 gcatatacac agggatatta aaatattcca aataatattt cattcaagtt ttcccccatc
    12181 aaattgggac atggatttct ccggtgaata ggcagagttg gaaactaaac aaatgttggt
    12241 tttgtgattt gtgaaattgt tttcaagtga tagttaaagc ccatgagata cagaacaaag
    12301 ctgctatttc gaggtctctt ggttatactc agaagcactt ctttgggttt ccctgcacta
    12361 tcctgatcat gtgctaggcc twccttaggc tgattgttgt tcaaataact taagtttcct
    12421 gtcaggtgat gtcatatgat ttcatatatc aaggcaaaac atgttatata tgttaaacat
    12481 ttgkacttaa tgtgaaagtt aggtctttgt gggttttgat tttaatttca aaacctgagc
    12541 taaataagtc attttacatg tcttacattt ggtgaattgt atattgtggt ttgcaggcaa
    12601 gactctctga cctagtaacc ctcctataga gcactttgct gggtcacaag tctaggagtc
    12661 aagcatttca ccttgaagtt gagacgtttt gttagtgtat actagttata tgttggagga
    12721 catgtttatc cagaagatat tcaggactat ttttgactgg gctaaggaat tgattctgat
    12781 tagcactgtt agtgagcatt gagtggcctt taggcttgaa ttggagtcac ttgtatatct
    12841 caaataatgc tggccttttt twaaaagccc ttgttcttta tcaccctgtt ttctacataa
    12901 tttttgttca aagaaatact tgtttggatc tccttttgac aacaatagca tgttttcaag
    12961 ccatattttt tttccttttt tttttttttt ttggtttttc gagacagggt ttctctgtat
    13021 agccctggct gtcctggaac tcactttgta gaccaggctg gcctcgaact cagaaatccg
    13081 cctgcctctg cctcctgagt gccgggatta aaggcgtgca ccaccacgcc tggctaagtt
    13141 ggatattttg tatataacta taaccaatac taactccact gggtggattt ttaattcagt
    13201 cagtagtctt aagtggtctt tattggccct tattaaaatc tactgttcac tctaacagag
    13261 gctgttggac tagtggsact aagcaacttc ctacggatat actagcagat aagggtcagg
    13321 gatagaaact agtctagcgt tttgtatacc taccagctta tactaccttg ttctgataga
    13381 aatatttagg acatctagct tatcgatccg tcgagggggg gcccggtacc cagcttttgt
    13441 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
    13501 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    13561 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    13621 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    13681 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    13741 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    13801 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    13861 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    13921 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    13981 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    14041 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    14101 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    14161 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    14221 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    14281 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    14341 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    14401 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    14461 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    14521 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    14581 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    14641 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    14701 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    14761 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    14821 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    14881 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    14941 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    15001 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    15061 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    15121 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    15181 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    15241 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    15301 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    15361 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    15421 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    15481 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    15541 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    15601 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
    15661 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
    15721 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
    15781 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
    15841 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
    15901 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
    15961 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
    16021 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
    16081 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    16141 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    16201 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
    16261 acgactcact atagggcgaa ttggagctcc a
//
