LOCUS       sequence-100375-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral cDNA/shRNA gateway destination vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Radulovich N, Leung L, Tsao MS
  TITLE     Modified gateway system for double shRNA expression and Cre/lox 
            based gene expression.
  JOURNAL   BMC Biotechnol. 2011 Mar 22;11:24.
  PUBMED    21418658
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011-03-22"; volume: "11"; pages: "24"
COMMENT     Sequence Label: pLKO.DEST.puro
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(90..96,97..114,115..120))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(145..269)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(306..554)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(364..383)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(534..554)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(852..896)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
       61 tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa
      121 gcctggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat
      181 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
      241 tctcgttcag cttttttgta caaacttgtt gatgggctcg aggatcgcgt gcatacctgc
      301 ggaccggtgt ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa
      361 gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca
      421 agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa
      481 ttaattccaa ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg
      541 ggaaataggc cctcggtgaa gggggcggcc gctcgaggct agtctcgtga tcgataccgt
      601 cgagatccgt tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct
      661 attccttcgg gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt
      721 gaatatccct gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct
      781 accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt ggttatgtta
      841 aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc attcttttct
      901 tgctggtttt gcgattcttc aattaaggag tgtattaagc ttg
//
