LOCUS       sequence-100376-        8886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral cDNA/shRNA gateway destination vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Radulovich N, Leung L, Tsao MS
  TITLE     Modified gateway system for double shRNA expression and Cre/lox 
            based gene expression.
  JOURNAL   BMC Biotechnol. 2011 Mar 22;11:24.
  PUBMED    21418658
REFERENCE   2  (bases 1 to 8886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011-03-22"; volume: "11"; pages: "24"
COMMENT     Sequence Label: pLKO.DEST.puro
FEATURES             Location/Qualifiers
     source          1..8886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    30..147
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        196..706
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     625..643
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             728..1327
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(728..747)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1224..1244
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1455..1688
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1760..1881
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1797..1816)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1851..1870
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1921..2056
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2076..2095)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2077..2095)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2105..2122)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2105..2121)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2114..2136)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2263..2718
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2350..2369)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2560..2581
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2744..2848
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2849..2917,2918..3709)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3067..3086)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3880..4468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4369..4388
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4835..4855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4870..4875,4876..4893,4894..4900)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4908..4924
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4913..4935
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4932..4948
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4932..4948
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4967..4987
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4969..4987
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5015..5241
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5242..5422
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5469..5594
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6087..6320
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6505..6549
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6847..7095
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6847..6867
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7018..7037
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    7132..7256
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7281..7286,7287..7304,7305..7311)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7365..8024
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7431..7450)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8366..8671
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8550..8569
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8712..8836)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
       61 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
      121 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
      181 tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca
      241 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg
      301 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc
      361 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg
      421 ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca
      481 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg
      541 gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt
      601 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc
      661 ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg atccaccgga
      721 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
      781 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
      841 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
      901 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
      961 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     1021 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     1081 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     1141 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     1201 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     1261 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     1321 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
     1381 atcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     1441 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     1501 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     1561 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     1621 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     1681 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     1741 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     1801 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1861 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     1921 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     1981 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     2041 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     2101 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     2161 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     2221 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     2281 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     2341 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     2401 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     2461 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     2521 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     2581 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     2641 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     2701 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2761 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2821 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     2881 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     2941 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3001 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3061 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3121 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3181 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3241 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3301 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3361 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3421 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3481 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3541 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3601 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3661 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     3721 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3781 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3841 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3901 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3961 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4021 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4081 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4141 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4201 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4261 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4321 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4381 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4441 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4501 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4561 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4621 cagcgagtca gtgagcgagg aagcggaaga gcgccctttg agtgagctga taccgctcgc
     4681 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     4741 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     4801 cccgactgga aagcgggcag tgagcgcaac gcaaaatgtg agttagctca ctcattaggc
     4861 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     4921 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     4981 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     5041 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     5101 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     5161 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     5221 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     5281 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     5341 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     5401 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     5461 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     5521 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     5581 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     5641 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     5701 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     5761 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     5821 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     5881 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     5941 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     6001 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     6061 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     6121 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     6181 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     6241 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     6301 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     6361 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     6421 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     6481 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     6541 gcaagtttgt ggaattggtt taacataacc aattggctgt ggtatataaa attattcata
     6601 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     6661 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     6721 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     6781 cgattagtga acggatctcg acggtatcga tcacgagact agcctcgagc ggccgccccc
     6841 ttcaccgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
     6901 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     6961 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     7021 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     7081 aaaggacgaa acaccggtcc gcaggtatgc acgcgatcct cgagcccatc aacaagtttg
     7141 tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
     7201 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg
     7261 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
     7321 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     7381 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     7441 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     7501 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     7561 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     7621 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     7681 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     7741 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     7801 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     7861 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     7921 cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta
     7981 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct
     8041 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
     8101 atactgatat gtatacccga agtatgtcaa aaagaggtgt gctatgaagc agcgtattac
     8161 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
     8221 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
     8281 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
     8341 gctgacgaga acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag
     8401 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
     8461 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
     8521 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
     8581 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
     8641 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
     8701 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
     8761 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
     8821 tcttgtacaa agtggttgat gggcggccgc tctagagggc ccaagcttac gcgtgaattc
     8881 gtcgac
//
