LOCUS       sequence-100382-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter construct for lineage tracing and mosaic analysis in 
            zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Collins RT, Linker C, Lewis J
  TITLE     MAZe: a tool for mosaic analysis of gene function in zebrafish.
  JOURNAL   Nat Methods. 2010 Mar;7(3):219-23. doi: 10.1038/nmeth.1423. Epub 
            2010 Feb 7.
  PUBMED    20139970
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1423"; journalName: "Nat Methods"; date: "2010-03"; 
            volume: "7"; issue: "3"; pages: "219-23"
COMMENT     Sequence Label: pMAZe
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    189..222
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    307..441
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(344..363)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     398..417
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    681..728
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     753..772
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 ctcccagttg ttcttcagac acttggcgca cttcggtttt tctttggagc acttgagctt
       61 tttaagtcgg caaatatcgc atgcttgttc gatagaagac agtagcttca tctttcagga
      121 ggcagcttag atccctctcg agcctctagg ggctgcagga attcgatggg ctgcaggaat
      181 tcgattaaat aacttcgtat aatgtatgct atacgaagtt atccctagaa ctagggccgc
      241 gaattaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      301 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      361 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
      421 gtatcttatc atgtctggat ctacgtaata cgactcacta tagttctaga ggctcgagag
      481 gaaatcaatc taaagtatat atgagtaacc tgaggctatg gcagggcctg ccgccccgac
      541 gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga
      601 aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg
      661 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg tgcgttttat tctgtctttt
      721 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
      781 ccccctaggg tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc
      841 ggcgtcccgg aaaacgattc cgaagcccaa cctttca
//
