LOCUS       sequence-100384-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Amphioxus GFPc1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Bomati EK, Haley JE, Noel JP, Deheyn DD
  TITLE     Spectral and structural comparison between bright and dim green 
            fluorescent proteins in Amphioxus.
  JOURNAL   Sci Rep. 2014 Jun 27;4:5469. doi: 10.1038/srep05469.
  PUBMED    24968921
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-06-27"; volume: "4:5469. doi"; pages: " 
            10.1038/srep05469"
COMMENT     Sequence Label: bfloGFPc1a
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(719..736)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(743..766)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agctttcaca
       61 ggtcatggaa agccttctgc cactccttaa ggttgatctc cgcgtcagag gccttgactt
      121 ccgtcttacg gaacacgaac atcggctgct tctggaggat ggtggccgct atcggcttgg
      181 cgaaggtgta gttggtcctc agcgtggcat ggtagcgttt gccgctggta gtagggcaag
      241 tccagtcaat ggtggagaca acggtggtgt cgtttgggaa gagcatggta gccacgctcc
      301 aatccacagc cgtgagcgat ttggtcatca cgggaccgtc agcagggaaa ccgctaccaa
      361 ccacatgaaa ctctcctttg atgtggcctc catcgtagga atatcggtag ttgccagtca
      421 gcgaggcgcc gtcttcaaac tgaatcgtac gatggactac gtaccccgag ccatcgtccg
      481 cagcggcctg gaaaggcgac atcccgtctg ggaagggcag gtactggtgg aagccgtacc
      541 cgatgttggg gaccacgatg tacggggaga acccgagggg acccttagtg gacttcactt
      601 ggatctcctc agaaccatcc ttcgggttgc ctttgccgct acccaccaag tcaaactcaa
      661 caccgttgat agagccatag acgtgcacct cgtgagtcgt tggaagaggc atgccatggg
      721 aaccacgcgg aaccagacca ccgtggtggt ggtggtggtg gtggtgtttc atggtatatc
      781 tccttcttaa agttaaaaca
//
