LOCUS       sequence-100389-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of conditionally active form of Cre recombinase
            (not as tightly regulated as pCAG-ERT2CreERT2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Controlled expression of transgenes introduced by in vivo 
            electroporation.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 
            10.1073/pnas.0610155104
  PUBMED    17209010
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2007-01-16- 16"; 
            volume: "104"; issue: "3"; pages: "1027-32"
COMMENT     Sequence Label: pCAG-CreERT2
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      291..300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(334..353)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(415..437)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 aagcatgttt agctggccca aatgttgctg gatagttttt actgccagac cgcgcgcctg
       61 aagatataga agataatcgc gaacatcttc aggttctgcg ggaaaccatt tccggttatt
      121 caacttgcac catgccgccc acgaccggca aacggacaga agcattttcc aggtatgctc
      181 agaaaacgcc tggcgatccc tgaacatgtc catcaggttc ttgcgaacct catcactcgt
      241 tgcatcgacc ggtaatgcag gcaaattttg gtgtacggtc agtaaattgg ccatggtggc
      301 ggctcagaat tctttgccaa aatgatgaga cagcacaata accagcacgt tgcccaggag
      361 ctgtaggaaa aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac
      421 acgccagaag ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg
      481 acagccccga ggctggagag ggagaagggg acggcggcgc ggcgacgcac gaaggccctc
      541 cccgcccatt tccttcctgc cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt
      601 gcggcggcgc ctcccagatt tcggctccgc acagatttgg gacaaaggaa gtccctgcgc
      661 cctctcgcac gattaccata aaaggcaatg gctgcggctc g
//
