LOCUS       sequence-100394-        8342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation vector pBDPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Koh TW, He Z, Gorur-Shandilya S, Menuz K, Larter NK, Stewart S, 
            Carlson JR
  TITLE     The Drosophila IR20a clade of ionotropic receptors are candidate 
            taste and pheromone receptors.
  JOURNAL   Neuron. 2014 Aug 20;83(4):850-65. doi: 10.1016/j.neuron.2014.07.012.
            Epub 2014 Aug 7.
  PUBMED    25123314
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.07.012"; journalName: "Neuron"; date: 
            "2014-08-20- 20"; volume: "83"; issue: "4"; pages: "850-65"
COMMENT     Sequence Label: pBDPR
FEATURES             Location/Qualifiers
     source          1..8342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    113..237
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(262..267,268..285,286..292)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             346..1005
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(412..431)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1347..1652
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1531..1550
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1693..1817)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(join(2865..3479,3550..3681,3885..4200,4262..4916,
                     4991..5264,5666..5737))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     6189..6207
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    6432..6501
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(6588..7176)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6668..6687)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7347..8138,8139..8207))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7970..7989
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8208..8312)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta aacgcccttt cgtcttcaag aattcgttta tcacaagttt
      121 gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
      181 tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt cactatggcg
      241 gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt
      301 ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
      361 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
      421 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
      481 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
      541 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
      601 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
      661 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
      721 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
      781 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
      841 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
      901 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
      961 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc
     1021 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
     1081 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
     1141 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
     1201 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
     1261 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
     1321 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
     1381 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
     1441 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
     1501 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     1561 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     1621 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
     1681 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
     1741 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
     1801 ttcttgtaca aagtggtgat aaacggccgg ccagatctgg taccggatcc ccgcgcggcc
     1861 gctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg
     1921 gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct
     1981 gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt
     2041 aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggagg cgcgccctag
     2101 ttccagtgaa atccaagcat tttctaaatt aaatgtattc ttattattat agttgttatt
     2161 tttgatatat ataaacaaca ctattatgcc caccattttt ttgagatgca tctacacaag
     2221 gaacaaacac tggatgtcac tttcagttca aattgtaacg ctaatcactc cgaacaggtc
     2281 acaaaaaatt accttaaaaa gtcataatat taaattagaa taaatatagc tgtgagggaa
     2341 atatatacaa atatattgga gcaaataaat tgtacataca aatatttatt actaatttct
     2401 attgagacga aatgaaccac tcggaaccat ttgagcgaac cgaatcgcgc ggaactaacg
     2461 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2521 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2581 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2641 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2701 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2761 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2821 atggtaaaaa aaaaaaacaa gcagttattt cggatatatg tcggctactc cttgcgtcgg
     2881 gcccgaagtc ttagagccag atatgcgagc acccggaagc tcacgatgag aatggccaga
     2941 cccacgtagt ccagcggcag atcggcggcg gagaagttaa gcgtctccag gatgaccttg
     3001 cccgaactgg ggcacgtggt gttcgacgat gtgcagctaa tttcgcccgg ctccacgtcc
     3061 gcccattggt taatcagcag accctcgttg gcgtaacgga accatgagag gtacgacaac
     3121 catttgaggt atactggcac cgagcccgag ttcaagaaga agccgccaaa gagcaggaat
     3181 ggtatgataa ccggcggacc cacagacagc gccatcgagg tcgaggagct ggcgcaggat
     3241 attagatatc cgaaggacgt tgacacattg gccaccagag tgaccagcgc caggcagttg
     3301 aagaagtgca gcactccggc ccgcagtccg atcatcggat aggcaatcgc cgtgaagacc
     3361 agtggcactg tgagaaaaag cggcaattcg gcaatcgttt tgcccagaaa gtatgtgtca
     3421 cagcgataaa gtcgacttcg ggcctccctc ataaaaactg gcagctctga ggtgaacacc
     3481 taaatcgaat cgattcatta gaaagttagt aaattattga aatgcaaatg tattctaaac
     3541 atgacttaca tttatcgtgg caaagacgtt ttgaaaggtc atgttggtca ggaagaggaa
     3601 gatggctccg ttgatattca tcacacccac ttgcgtgagt tgttggccca aaaagatgag
     3661 gccaatcaag atggcaacca tctgcaaatt aaaatgttac tcgcatctca ttaatattcg
     3721 cgagttaaat gaaatttatt tatcttctgc aaaactataa actatacatc tcattgaaaa
     3781 aaactaagaa gggtgtggaa tcaggcaatt ctatctaaaa tctagcgaat ttgtttccaa
     3841 gaattgtaag cgttatatca tttgtttcca ctggaaccac tcaccgttgt ctgaataagt
     3901 cgcactttta cgaggagtgg ttccttgagc accgacagcc aggatcgcca caggaccgcc
     3961 cggaactgca tgaaccaggt ggccttgtag gtgtacccat tctccggctg ctccagtggc
     4021 ttctccagat ttttggtggc caacaactgc tccatatccc gggctacttt gctaatggca
     4081 aaattgtcgc atatcttggc gatccgatca cgggactcga tctcccgtcc gggcacaacg
     4141 gccaacacct gtacgtaaaa gtccgccgga ttgtagttgg taggacactg ggcacccacg
     4201 ctggatagga gttgagatgt tatgtaatac tagataccct taataaacac atcgaactca
     4261 ctaggaaaag aagtcgacgg cttcgctggg agtgcccaag aaagctaccc tgccctcggc
     4321 catcagaagg atcttgtcaa agagctcaaa cagctcggaa gacggctgat gaatggtcag
     4381 gatgacggtc ttgcccttct gcgacagctt cttcagcacc tggacgacgc tgtgggcggt
     4441 aaaggagtcc agtccggagg tgggctcatc gcagatcaga agcggcggat cggttagagc
     4501 ctcggaggcg aatgccagac gcttcctttc tccgccggac agacctttca ccctgccggg
     4561 cacaccgatg atcgtgtgct gacatttgct gagcgaaagc tcctggatca cctgatccac
     4621 gcgggccact cgctgccgat aggtcagatg tcgtggcatc cgcaccatgg cttggaaaat
     4681 caggtgttcc ctggccgtta gggagccgat aaagaggtca tcctgctgga cataggcgca
     4741 cctggcctgc atctccttgg cgtccacagg ttggccattg agcagtcgca tcccggatgg
     4801 cgatacttgg atgccctgcg gcgatcgaaa ggcaagggca ttcagcaggg tcgtctttcc
     4861 ggcaccggaa ctgcccatca cggccaaaag ttcgcccgga taggccacgc cgcaaactga
     4921 gtttcaaatt ggtaattgga ccctttatta agatttcaca cagatcagcc gactgcgaat
     4981 agaaactcac cgttcttgag caaatgtttc ctgggcgccg gtatgtgtcg ctcgttgcag
     5041 aatagtccgc gtgtccggtt gaccagctgc cgccatccgg agcccggctg attgaccgcc
     5101 ccaaagatgt ccatattgtg ccaggcatag gtgaggttct cggctagttg gccgctccct
     5161 gaaccggagt cctccggcgg actgggtggc cggagcgtgc cgtagttttt ggcctgcccg
     5221 aagccctggt taatgcagct ctgcgaagcc gctccgctgt caccctgcaa tgatagggga
     5281 tctcaaatat caactactag cgttatgctc atctaacccc gaacaaaaag taccccgaag
     5341 tatcctacga agtaggttta tacttttatt tattttttgt gcatctagga tcagcttaaa
     5401 atatctggtt gttatatttt ttgtaaaaaa gaatatagtc gaaaatgaat gcctttagat
     5461 gtcttgatca tgatatgatc tcaaaaattg tcttatatag cgagaacagc taccagaata
     5521 atctgtttcg tgtcactatt tgtttgtgca attgcggttt gggatttttg tgggtcgcag
     5581 ttctcacgcc gcatacaatt tgatgttgca atcgcagttc ctatagatca agtgaactta
     5641 agatgtatgc acatgtacta ctcacattgt tcagatgctc ggcagatggg tgtttgctgc
     5701 ctccgcgaat taatagctcc tgatcctctt ggcccattgc cgggattttt cacactttcc
     5761 cctgcttacc cacccaaaac caatcaccac cccaatcact caaaaaacaa acaaaaataa
     5821 gaagcgagag gagttttggc acagcacttt gtgtttaatt gatggcgtaa accgcttgga
     5881 gcttcgtcac gaaaccgctg acaaaatgca actgaaggcg gacattgacg ctacgtaacg
     5941 ctacaaacgg tggcgaaaga gatagcggac gcagcggcga aagagacggc gatatttctg
     6001 tggacagaga aggaggcaaa cagcgctgac tttgagtgga atgtcatttt gagtgagagg
     6061 taatcgaaag aacctggtac atcaaatacc cttggatcga agtaaattta aaactgatca
     6121 gataagttca atgatatcca gtgcagtaaa aaaaaaaaat gtttttttta tctactttcc
     6181 gcaaaaatgg gttttattaa cttacataca tactaggcgc gcccatatgt tcggcttgtc
     6241 gacatgcccg ccgtgaccgt cgagaacccg ctgacgctgc cccgcgtatc cgcacccgcc
     6301 gacgccgtcg cacgtcccgt gctcaccgtg accaccgcgc ccagcggttt cgagggcgag
     6361 ggcttcccgg tgcgccgcgc gttcgccggg atcaactacc gccacctcga cccgttcatc
     6421 atgatggacc agatgggtga ggtggagtac gcgcccgggg agcccaaggg cacgccctgg
     6481 cacccgcacc gcggcttcga gaccgtgacc tacatcgtcg acggtaacat gtgagcaaaa
     6541 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6601 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6661 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6721 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6781 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6841 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6901 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6961 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7021 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7081 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7141 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7201 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7261 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7321 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7381 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7441 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7501 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7561 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7621 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7681 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7741 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7801 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7861 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7921 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7981 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8041 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8101 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8161 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8221 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8281 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgag
     8341 tc
//
