LOCUS       sequence-100399-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A fluorescent reporter of AMPK activity and cellular energy stress.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Tsou P, Zheng B, Hsu CH, Sasaki AT, Cantley LC
  TITLE     A fluorescent reporter of AMPK activity and cellular energy stress.
  JOURNAL   Cell Metab. 2011 Apr 6;13(4):476-86.
  PUBMED    21459332
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Metab."; date: "2011-04-6"; volume: "13(4)"; pages: "476-86"
COMMENT     Sequence Label: ampkar
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     470..491
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(597..618)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(858..877)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tttcaagact gtcaaataag catttccaaa tactactagg agaagacggt aaccttttat
       61 tgaatgacat ttccactaat gggacctggt taaatgggca aaaagtcgag aagaacagca
      121 atcagttact gtctcaaggt gatgaaataa ccgttggtgt aggcgtggaa tcagatattt
      181 tatctctggt cattttcata aacgacaaat ttaagcagtg cctcgagcag aacaaagttg
      241 atcgctctgc aggtaagcca ggcagcggcg agggcagcac caagatgcgt cgtgtcgcca
      301 ccctggtgga cctcggcacc ggcggcagcg agctcatggg cggcgtgcag ctcgccgacc
      361 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      421 tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      481 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggaggta
      541 ccggtggatc tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      601 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      661 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
      721 cctggcccac cctcgtgacc accctgggct acggccttca gtgcttcgcc cgctaccccg
      781 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      841 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      901 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
      961 tc
//
