LOCUS       sequence-100403-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Renilla SV40 promoter (no enhancer) reporter gene for normalization 
            of luciferase experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Chen X, Prywes R
  TITLE     Serum-induced expression of the cdc25A gene by relief of 
            E2F-mediated repression.
  JOURNAL   Mol Cell Biol. 1999 Jul . 19(7):4695-702.
  PUBMED    10373518
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Jul . 19(7):4695-702."
COMMENT     Sequence Label: pRL-SV40P
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        142..338
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      189..324
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          408..540
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     523..542
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        585..603
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tggctcgaca gatctgcgat ctgcatctca attagtcagc aaccatagtc ccgcccctaa
      181 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catcgctgac
      241 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
      301 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttgattct tctgacacaa
      361 cagtctcgaa cttaagctgc agaagttggt cgtgaggcac tgggcaggta agtatcaagg
      421 ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct
      481 tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag
      541 gtgtccactc ccagttcaat tacagctctt aaggctagag tacttaatac gactcactat
      601 aggctagcca ccatgacttc gaaagtttat gatccagaac aaaggaaacg gatgataact
      661 ggtccgcagt ggtgggccag atgtaaacaa atgaatgttc ttgattcatt tattaattat
      721 tatgattcag aaaaacatgc agaaaatgct gttatttttt tacatggtaa cgcggcctct
      781 tcttatttat ggcgacatgt tgtgccacat attgagccag tagcgcggtg tattatacca
      841 gaccttattg gtatgggcaa atcaggcaaa tctggtaatg gttcttatag gttacttgat
      901 cattacaaat atcttactgc atggtttgaa cttcttaatt taccaaagaa gatcattttt
      961 gtcggccatg attgggg
//
