LOCUS       sequence-100417-       10512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The human DPYD was used to study the effect of DPYD expression on 
            EMT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10512)
  AUTHORS   Shaul YD, Freinkman E, Comb WC, Cantor JR, Tam WL, Thiru P, Kim D, 
            Kanarek N, Pacold ME, Chen WW, Bierie B, Possemato R, Reinhardt F, 
            Weinberg RA, Yaffe MB, Sabatini DM
  TITLE     Dihydropyrimidine accumulation is required for the 
            epithelial-mesenchymal transition.
  JOURNAL   Cell. 2014 Aug 28;158(5):1094-109. doi: 10.1016/j.cell.2014.07.032.
  PUBMED    25171410
REFERENCE   2  (bases 1 to 10512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.07"; journalName: "Cell"; date: "2014-08-28- 
            28"; volume: "158"; issue: "5"; pages: "1094-109"
COMMENT     Sequence Label: DPYD-FLAG
FEATURES             Location/Qualifiers
     source          1..10512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3482..3547
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    3595..3712
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3761..4271
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4190..4208
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4293..4892
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4293..4312)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4789..4809
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5020..5253
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5325..5446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5362..5381)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5416..5435
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5486..5621
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5641..5660)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5642..5660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5670..5687)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5670..5686)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5679..5701)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5828..6283
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5915..5934)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6125..6146
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6309..6413
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6414..6482,6483..7274)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6632..6651)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7445..8033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7934..7953
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8187..8204
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8321..8342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8357..8362,8363..8380,8381..8387)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8395..8411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8400..8422
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8419..8435
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8419..8435
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8454..8474
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8456..8474
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8502..8728
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8729..8909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8956..9081
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9574..9807
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9992..10036
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     10334..10354
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg tttaattaag tcgctatggc ccctgtgctc agtaaggact cggcggacat
      421 cgagagtatc ctggctttaa atcctcgaac acaaactcat gcaactctgt gttccacttc
      481 ggccaagaaa ttagacaaga aacattggaa aagaaatcct gataagaact gctttaattg
      541 tgagaagctg gagaataatt ttgatgacat caagcacacg actcttggtg agcgaggagc
      601 tctccgagaa gcaatgagat gcctgaaatg tgcagatgcc ccgtgtcaga agagctgtcc
      661 aactaatctt gatattaaat cattcatcac aagtattgca aacaagaact attatggagc
      721 tgctaagatg atattttctg acaacccact tggtctgact tgtggaatgg tatgtccaac
      781 ctctgatctt tgtgtaggtg gatgcaattt atatgccact gaagagggac ccattaatat
      841 tggtggattg cagcaatttg ctactgaggt attcaaagca atgagtatcc cacagatcag
      901 aaatccttcg ctgcctcccc cagaaaaaat gtctgaagcc tattctgcaa agattgctct
      961 ttttggtgct gggcctgcaa gtataagttg tgcttccttt ttggctcgat tggggtactc
     1021 tgacatcact atatttgaaa aacaagaata tgttggtggt ttaagtactt ctgaaattcc
     1081 tcagttccgg ctgccgtatg atgtagtgaa ttttgagatt gagctaatga aggaccttgg
     1141 tgtaaagata atttgcggta aaagcctttc agtgaatgaa atgactctta gcactttgaa
     1201 agaaaaaggc tacaaagctg ctttcattgg aataggtttg ccagaaccca ataaagatgc
     1261 catcttccaa ggcctgacgc aggaccaggg gttttataca tccaaagact ttttgccact
     1321 tgtagccaaa ggcagtaaag caggaatgtg cgcctgtcac tctccattgc catcgatacg
     1381 gggagtcgtg attgtacttg gagctggaga cactgccttt gactgtgcaa catctgctct
     1441 acgttgtgga gctcgccgtg tgttcatcgt cttcagaaaa ggctttgtta atataagagc
     1501 tgtccctgag gagatggaac ttgctaagga agaaaagtgt gaatttctgc cattcctgtc
     1561 cccacggaag gttatagtaa aaggtgggag aattgttgct atgcagtttg ttcggacaga
     1621 gcaagatgaa actggaaaat ggaatgaaga tgaagatcag atggtccatc tgaaagccga
     1681 tgtggtcatc agtgcctttg gttcagttct gagtgatcct aaagtaaaag aagccttgag
     1741 ccctataaaa tttaacagat ggggtctccc agaagtagat ccagaaacta tgcaaactag
     1801 tgaagcatgg gtatttgcag gtggtgatgt cgttggtttg gctaacacta cagtggaatc
     1861 ggtgaatgat ggaaagcaag cttcttggta cattcacaaa tacgtacagt cacaatatgg
     1921 agcttccgtt tctgccaagc ctgaactacc cctcttttac actcctattg atctggtgga
     1981 cattagtgta gaaatggccg gattgaagtt tataaatcct tttggtcttg ctagcgcaac
     2041 tccagccacc agcacatcaa tgattcgaag agcttttgaa gctggatggg gttttgccct
     2101 caccaaaact ttctctcttg ataaggacat tgtgacaaat gtttccccca gaatcatccg
     2161 gggaaccacc tctggcccca tgtatggccc tggacaaagc tcctttctga atattgagct
     2221 catcagtgag aaaacggctg catattggtg tcaaagtgtc actgaactaa aggctgactt
     2281 tccagacaac attgtgattg ctagcattat gtgcagttac aataaaaatg actggacgga
     2341 acttgccaag aagtctgagg attctggagc agatgccctg gagttaaatt tatcatgtcc
     2401 acatggcatg ggagaaagag gaatgggcct ggcctgtggg caggatccag agctggtgcg
     2461 gaacatctgc cgctgggtta ggcaagctgt tcagattcct ttttttgcca agctgacccc
     2521 aaatgtcact gatattgtga gcatcgcaag agctgcaaag gaaggtggtg ccaatggcgt
     2581 tacagccacc aacactgtct caggtctgat gggattaaaa tctgatggca caccttggcc
     2641 agcagtgggg attgcaaagc gaactacata tggaggagtg tctgggacag caatcagacc
     2701 tattgctttg agagctgtga cctccattgc tcgtgctctg cctggatttc ccattttggc
     2761 tactggtgga attgactctg ctgaaagtgg tcttcagttt ctccatagtg gtgcttccgt
     2821 cctccaggta tgcagtgcca ttcagaatca ggatttcact gtgatcgaag actactgcac
     2881 tggcctcaaa gccctgcttt atctgaaaag cattgaagaa ctacaagact gggatggaca
     2941 gagtccagct actgtgagtc accagaaagg gaaaccagtt ccacgtatag ctgaactcat
     3001 ggacaagaaa ctgccaagtt ttggacctta tctggaacag cgcaagaaaa tcatagcaga
     3061 aaacaagatt agactgaaag aacaaaatgt agctttttca ccacttaaga gaaactgttt
     3121 tatccccaaa aggcctattc ctaccatcaa ggatgtaata ggaaaagcac tgcagtacct
     3181 tggaacattt ggtgaattga gcaacgtaga gcaagttgtg gctatgattg atgaagaaat
     3241 gtgtatcaac tgtggtaaat gctacatgac ctgtaatgat tctggctacc aggctataca
     3301 gtttgatcca gaaacccacc tgcccaccat aaccgacact tgtacaggct gtactctgtg
     3361 tctcagtgtt tgccctattg tcgactgcat caaaatggtt tccaggacaa caccttatga
     3421 accaaagaga ggcgtaccct tatctgtgaa tccggtgtgt ggaggcagcg cggccgcagg
     3481 cgactacaaa gaccatgacg gtgattataa agatcatgac attgattaca aggatgacga
     3541 tgacaagtaa taagaattct cgacctcgag acaaatggca gtattcatcc acaattttaa
     3601 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     3661 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     3721 ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg ttgcgccttt
     3781 tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac
     3841 gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga
     3901 tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg
     3961 aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta
     4021 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
     4081 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
     4141 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat
     4201 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc
     4261 tctctcccca gggggatcca ccggagctta ccatgaccga gtacaagccc acggtgcgcc
     4321 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     4381 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     4441 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     4501 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     4561 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     4621 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     4681 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     4741 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     4801 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     4861 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
     4921 ccgaaaggag cgcacgaccc catgcatcgg tacctttaag accaatgact tacaaggcag
     4981 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5041 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     5101 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5161 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5221 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     5281 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     5341 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5401 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     5461 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5521 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5581 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     5641 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     5701 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5761 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5821 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5881 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5941 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6001 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6061 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6121 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6181 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6241 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     6301 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7261 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7321 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7381 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7441 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7501 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7561 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7621 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7681 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7741 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7801 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7861 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7921 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7981 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8041 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8101 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8161 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8221 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8281 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     8341 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     8401 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     8461 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     8521 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     8581 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     8641 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     8701 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     8761 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8821 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8881 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     8941 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     9001 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     9061 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     9121 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     9181 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     9241 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     9301 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     9361 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     9421 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     9481 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     9541 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     9601 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     9661 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     9721 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     9781 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     9841 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     9901 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     9961 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    10021 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    10081 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    10141 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
    10201 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
    10261 atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc ctcgagcggc
    10321 cgcccccttc accgagggcc tatttcccat gattccttca tatttgcata tacgatacaa
    10381 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
    10441 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
    10501 aatggactat ca
//
