LOCUS       sequence-100418-        8994 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To study the effect of DPYS activity on EMT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   Shaul YD, Freinkman E, Comb WC, Cantor JR, Tam WL, Thiru P, Kim D, 
            Kanarek N, Pacold ME, Chen WW, Bierie B, Possemato R, Reinhardt F, 
            Weinberg RA, Yaffe MB, Sabatini DM
  TITLE     Dihydropyrimidine accumulation is required for the 
            epithelial-mesenchymal transition.
  JOURNAL   Cell. 2014 Aug 28;158(5):1094-109. doi: 10.1016/j.cell.2014.07.032.
  PUBMED    25171410
REFERENCE   2  (bases 1 to 8994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.07"; journalName: "Cell"; date: "2014-08-28- 
            28"; volume: "158"; issue: "5"; pages: "1094-109"
COMMENT     Sequence Label: DPYS-FLAG
FEATURES             Location/Qualifiers
     source          1..8994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1964..2029
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2077..2194
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2243..2753
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2672..2690
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2775..3374
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2775..2794)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3271..3291
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3502..3735
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3807..3928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3844..3863)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3898..3917
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3968..4103
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4123..4142)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4124..4142)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4152..4169)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4152..4168)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4161..4183)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4310..4765
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4397..4416)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4607..4628
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4791..4895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4896..4964,4965..5756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5114..5133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5927..6515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6416..6435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6669..6686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6803..6824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6839..6844,6845..6862,6863..6869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6877..6893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6882..6904
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6901..6917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6901..6917
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6936..6956
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6938..6956
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6984..7210
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7211..7391
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7438..7563
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8056..8289
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8474..8518
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     8816..8836
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg tttaattaac tcgctatggc ggcgccctcg cggctcctga tccgcggggg
      421 tcgcgtggtc aacgatgact tctcggaggt ggccgacgtg ctggtggagg acggcgtggt
      481 gcgggcactc gggcacgacc tgctgcctcc cgggggcgct cctgcggggc tgcgggtcct
      541 cgacgccgcc ggcaagctcg tcctgcccgg aggcatcgac acacacacgc acatgcagtt
      601 ccccttcatg ggctcgcggt ccatcgacga cttccaccag ggcaccaagg ctgctctctc
      661 aggaggcacc accatgatta ttgatttcgc cattcctcag aaaggtggct ccctcattga
      721 ggccttcgag acctggcgaa gctgggctga tcccaaagtt tgctgcgact acagccttca
      781 tgtggcagtg acgtggtgga gtgaccaggt taaagaagaa atgaaaatcc ttgtgcaaga
      841 taaaggtgtt aactctttca agatgtttat ggcctataaa gatctgtaca tggtgacaga
      901 cctggagctg tacgaagcct tctctcggtg caaggaaatt ggagcaattg cccaggtcca
      961 tgcggaaaat ggagacttaa ttgcagaggg agcaaagaag atgttggctc tggggataac
     1021 aggccctgag ggccacgagc tgtgccgccc agaggcagtg gaggcagagg ccacgctgag
     1081 agccatcacc atagccagcg ctgtgaactg tcctctctac attgtgcatg tgatgagcaa
     1141 gtctgcagct aaggtgatag cggatgcaag gagagatggg aaggtggtct atggtgaacc
     1201 catagcagcc agtcttggca cagatggcac tcactactgg aataaagaat ggcaccatgc
     1261 agcccaccat gtcatgggtc cacctttgcg accagacccc tcaacacccg acttcctcat
     1321 gaatctgttg gctaatgatg atctaaccac aacagggact gataactgca ctttcaacac
     1381 ctgccagaaa gctcttggga aggatgattt taccaagatc cccaatgggg tgaatggtgt
     1441 tgaagatcgg atgtccgtaa tatgggaaaa aggcgtgcat agtggtaaaa tggatgaaaa
     1501 cagatttgtg gcagttacca gcacaaatgc agccaaaatt tttaatctct atccaagaaa
     1561 aggaagaata gctgtaggat cagatgctga cattgttatt tgggacccaa aaggcacaag
     1621 gactatctca gcaaaaactc atcatcaggc tgttaacttc aacattttcg agggcatggt
     1681 ttgccacggg gtgccccttg tgactatttc aagaggcaaa gtggtatatg aagccggagt
     1741 gttcagtgtc acggcaggag atgggaagtt tattcctcga aaaccatttg ctgaatatat
     1801 ttacaaacga ataaagcagc gagaccggac ttgcacacct acccctgtgg agcgtgcacc
     1861 ctataaggga gaagtcgcca cactgaaatc cagagtgaca aaagaagatg ccacagcagg
     1921 gaccaggaaa caggcccacc ccggaggcag cgcggccgca ggcgactaca aagaccatga
     1981 cggtgattat aaagatcatg acattgatta caaggatgac gatgacaagt aataagaatt
     2041 ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     2101 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2161 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     2221 tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc agccctgggt
     2281 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     2341 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     2401 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     2461 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     2521 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     2581 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     2641 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     2701 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc
     2761 caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     2821 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     2881 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     2941 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     3001 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     3061 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     3121 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     3181 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     3241 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     3301 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     3361 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     3421 cccatgcatc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     3481 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     3541 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3601 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3661 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     3721 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     3781 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     3841 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3901 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     3961 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4021 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4081 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     4141 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     4201 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4261 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     4321 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4381 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4441 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4501 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4561 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4621 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4681 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4741 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4801 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4861 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4921 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4981 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5041 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5101 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5161 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5221 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5281 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5341 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5401 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5461 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5521 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5581 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5641 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5701 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5761 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5821 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5881 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5941 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6001 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6061 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6121 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6181 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6241 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6301 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6361 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6421 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6481 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6541 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6601 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6661 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6721 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6781 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6841 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6901 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     6961 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     7021 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     7081 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     7141 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     7201 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7261 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7321 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7381 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     7441 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     7501 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     7561 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     7621 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     7681 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     7741 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     7801 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     7861 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     7921 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     7981 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     8041 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     8101 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     8161 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     8221 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     8281 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     8341 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     8401 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     8461 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     8521 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     8581 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     8641 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     8701 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     8761 cggatctcga cggtatcgat cacgagacta gcctcgagcg gccgccccct tcaccgaggg
     8821 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
     8881 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
     8941 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atca
//
