LOCUS       sequence-100438-       11381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL
            expressed under AtUBQ10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11381)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 11381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMU-ERr
FEATURES             Location/Qualifiers
     source          1..11381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    239..263
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(356..608)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(648..1442)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(759..778)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1369..1388
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1476..1655)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1773..1795
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1792..1808
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1792..1808
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1828..1848
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3835..3859
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3961..4668
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4374..4393
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(4697..4721)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4952..4972)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4980..4997)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4980..4996)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4989..5011)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5085..5109
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6409..7038
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7472..8539
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8605..8799
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9035..9057)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9143..9283
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9198..9217
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9298..9315)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9469..10057)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9549..9568)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10301..11092)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctcatg tatgataatt
     1741 cgagggtacc cggggatcct ctagagggcc cgagcggata acaatttcac acaggaaaca
     1801 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgag tctagctcaa
     1861 cagagctttt aacccaaatt ggtacaatag aatacaactt tagatcataa ttctcaaaag
     1921 aaagagattc cttagctatt ctatctgcca ctccatttcc ttctcggctt gtatgcacaa
     1981 gcataaaatc ctcaaacttg ctaagtagat actttatgtc ttggataatt ggattgagac
     2041 ttgacaagca taactttcat gtaaccaaag acacaagttg ctgagaatcc acctcaaaaa
     2101 tgatcttcct ataattgaat cgggataatg acagcacagc ccatctaaga gcctccactt
     2161 ctacttccag cacgcttctt acttttacca cagctcttgc acctaaccat aacaccttcc
     2221 ctgtatgatc gcgaagcacc caccctaagc cacattttaa tccttctgtt ggccatgccc
     2281 catcaaagtt gcacttaacc caagattgtg gtggagcttc ccatgtttct cgtctgtccc
     2341 gacggtgttg tggttggtgc tttccttaca ttctgagcct ctttccttct aatccactca
     2401 tctgcatctt cttgtgtcct tactaatacc tcattggttc caaattccct ccctttaagc
     2461 accagctcgt ttctgttctt ccacagcctc ccaagtatcc aagggactaa agcctccaca
     2521 ttcttcagat caggatattc ttgtttaaga tgttgaactc tatggaggtt tgtatgaact
     2581 gatgatctag gaccggataa gttcccttct tcatagcgaa cttattcaaa gaatgttttg
     2641 tgtatcattc ttgttacatt gttattaatg aaaaaatatt attggtcatt ggactgaaca
     2701 cgagtgttaa atatggacca ggccccaaat aagatccatt gatatatgaa ttaaataaca
     2761 agaataaatc gagtcaccaa accacttgcc ttttttaacg agacttgttc accaacttga
     2821 tacaaaagtc attatcctat gcaaatcaat aatcatacaa aaatatccaa taacactaaa
     2881 aaattaaaag aaatggataa tttcacaata tgttatacga taaagaagtt acttttccaa
     2941 gaaattcact gattttataa gcccacttgc attagataaa tggcaaaaaa aaacaaaaag
     3001 gaaaagaaat aaagcacgaa gaattctaga aaatacgaaa tacgcttcaa tgcagtggga
     3061 cccacggttc aattattgcc aattttcagc tccaccgtat atttaaaaaa taaaacgata
     3121 atgctaaaaa aatataaatc gtaacgatcg ttaaatctca acggctggat cttatgacga
     3181 ccgttagaaa ttgtggttgt cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc
     3241 ggcacacacg agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat
     3301 aaggcaatta gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta
     3361 ttattagcta ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct
     3421 tcttcttctt ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa
     3481 tttctcaaaa tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct
     3541 gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat
     3601 gttctttggt ttagattctg ttaatcttag atcgaagacg attttctggg tttgatcgtt
     3661 agatatcatc ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat
     3721 ttgagttttg tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt
     3781 ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acaggcaagt
     3841 ttgtacaaaa aagcaggctt cgagaagatg aaggtacagg agggtttgtt cgtggtggct
     3901 gttttctacc ttgcttatac gcagctagtc aaggggcaac ctcgcaagga gtgcggatcc
     3961 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     4021 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     4081 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     4141 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     4201 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     4261 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     4321 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     4381 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     4441 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     4501 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     4561 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     4621 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagag atctcatgac
     4681 gagctgtaac tgcaggaccc agctttcttg tacaaagtgg aataagcggc catgctagag
     4741 tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata
     4801 atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt
     4861 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     4921 ttctaattcc taaaaccaaa atccagtgac gcaactttat tatacatagt tgataattca
     4981 ctggccgtcg ttttacaacg tcgtgactgg gaaaacgagc tcaagcttag cttgagcttg
     5041 gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg
     5101 cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa agggcgtgaa
     5161 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
     5221 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     5281 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     5341 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     5401 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     5461 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     5521 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     5581 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     5641 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     5701 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     5761 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     5821 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     5881 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     5941 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     6001 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     6061 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     6121 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     6181 cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc
     6241 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     6301 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     6361 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     6421 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     6481 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     6541 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     6601 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     6661 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     6721 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     6781 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     6841 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     6901 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     6961 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     7021 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     7081 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     7141 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     7201 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     7261 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     7321 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     7381 ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact
     7441 ggaaccccca agcccgagga atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat
     7501 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg
     7561 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat
     7621 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa
     7681 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag
     7741 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga
     7801 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat
     7861 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat
     7921 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc
     7981 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga
     8041 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa
     8101 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa
     8161 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga
     8221 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga
     8281 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc
     8341 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt
     8401 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga
     8461 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga
     8521 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga
     8581 aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta
     8641 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca
     8701 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa
     8761 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc
     8821 cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc
     8881 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct
     8941 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac
     9001 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     9061 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     9121 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     9181 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     9241 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     9301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9901 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcatgatat
    10141 atctcccaat ttgtgtaggg cttattatgc acgcttaaaa ataataaaag cagacttgac
    10201 ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc taatcgcttg agttaacgcc
    10261 ggcgaagcgg cgtcggcttg aacgaatttc tagctagaca ttatttgccg actaccttgg
    10321 tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc
    10381 gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg
    10441 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta
    10501 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt
    10561 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag
    10621 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg
    10681 cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa
    10741 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg
    10801 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc
    10861 caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa
    10921 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca
    10981 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc
    11041 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccccc atgatgttta
    11101 actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac
    11161 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa
    11221 aaaaacatgt cataacaaga agccatgaaa accgccactg cgccgttacc accgctgcgt
    11281 tcggtcaagg ttctggacca gttgcgtgac ggcagttacg ctacttgcat tacagcttac
    11341 gaaccgaacg aggcttatgt ccactgggtt cgtgcccgaa t
//
