LOCUS       sequence-100454-       11435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed 
            under AtUBQ10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 11435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMU-GAr
FEATURES             Location/Qualifiers
     source          1..11435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    239..263
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(356..608)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(648..1442)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(759..778)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1369..1388
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1476..1655)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1773..1795
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1792..1808
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1792..1808
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1828..1848
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3835..3859
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4039..4749
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4452..4471
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(4751..4775)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5006..5026)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5034..5051)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5034..5050)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5043..5065)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5139..5163
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6463..7092
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7526..8593
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8659..8853
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9089..9111)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9197..9337
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9252..9271
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9352..9369)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9523..10111)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9603..9622)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10355..11146)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctcatg tatgataatt
     1741 cgagggtacc cggggatcct ctagagggcc cgagcggata acaatttcac acaggaaaca
     1801 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgag tctagctcaa
     1861 cagagctttt aacccaaatt ggtacaatag aatacaactt tagatcataa ttctcaaaag
     1921 aaagagattc cttagctatt ctatctgcca ctccatttcc ttctcggctt gtatgcacaa
     1981 gcataaaatc ctcaaacttg ctaagtagat actttatgtc ttggataatt ggattgagac
     2041 ttgacaagca taactttcat gtaaccaaag acacaagttg ctgagaatcc acctcaaaaa
     2101 tgatcttcct ataattgaat cgggataatg acagcacagc ccatctaaga gcctccactt
     2161 ctacttccag cacgcttctt acttttacca cagctcttgc acctaaccat aacaccttcc
     2221 ctgtatgatc gcgaagcacc caccctaagc cacattttaa tccttctgtt ggccatgccc
     2281 catcaaagtt gcacttaacc caagattgtg gtggagcttc ccatgtttct cgtctgtccc
     2341 gacggtgttg tggttggtgc tttccttaca ttctgagcct ctttccttct aatccactca
     2401 tctgcatctt cttgtgtcct tactaatacc tcattggttc caaattccct ccctttaagc
     2461 accagctcgt ttctgttctt ccacagcctc ccaagtatcc aagggactaa agcctccaca
     2521 ttcttcagat caggatattc ttgtttaaga tgttgaactc tatggaggtt tgtatgaact
     2581 gatgatctag gaccggataa gttcccttct tcatagcgaa cttattcaaa gaatgttttg
     2641 tgtatcattc ttgttacatt gttattaatg aaaaaatatt attggtcatt ggactgaaca
     2701 cgagtgttaa atatggacca ggccccaaat aagatccatt gatatatgaa ttaaataaca
     2761 agaataaatc gagtcaccaa accacttgcc ttttttaacg agacttgttc accaacttga
     2821 tacaaaagtc attatcctat gcaaatcaat aatcatacaa aaatatccaa taacactaaa
     2881 aaattaaaag aaatggataa tttcacaata tgttatacga taaagaagtt acttttccaa
     2941 gaaattcact gattttataa gcccacttgc attagataaa tggcaaaaaa aaacaaaaag
     3001 gaaaagaaat aaagcacgaa gaattctaga aaatacgaaa tacgcttcaa tgcagtggga
     3061 cccacggttc aattattgcc aattttcagc tccaccgtat atttaaaaaa taaaacgata
     3121 atgctaaaaa aatataaatc gtaacgatcg ttaaatctca acggctggat cttatgacga
     3181 ccgttagaaa ttgtggttgt cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc
     3241 ggcacacacg agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat
     3301 aaggcaatta gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta
     3361 ttattagcta ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct
     3421 tcttcttctt ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa
     3481 tttctcaaaa tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct
     3541 gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat
     3601 gttctttggt ttagattctg ttaatcttag atcgaagacg attttctggg tttgatcgtt
     3661 agatatcatc ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat
     3721 ttgagttttg tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt
     3781 ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acaggcaagt
     3841 ttgtacaaaa aagcaggctt catggcgaga gggagcagat cagtgggtag cagcagcagc
     3901 aaatggaggt actgcaaccc tactagttac ttgaagcgcc caaagcgtct tgctctgctc
     3961 ttcatcgttt tcgtttgtgt ctctttcgtt ttctgggacc gtcaaactga cgtctcgagg
     4021 accggtcccg ggggatccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     4081 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     4141 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     4201 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     4261 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     4321 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     4381 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     4441 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     4501 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     4561 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     4621 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     4681 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     4741 tacaagtaag acccagcttt cttgtacaaa gtggaataag cggccatgct agagtccgca
     4801 aaaatcacca gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt
     4861 gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat
     4921 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
     4981 ttcctaaaac caaaatccag tgacgcaact ttattataca tagttgataa ttcactggcc
     5041 gtcgttttac aacgtcgtga ctgggaaaac gagctcaagc ttagcttgag cttggatcag
     5101 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
     5161 aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt
     5221 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
     5281 ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt
     5341 cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct
     5401 tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga
     5461 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg
     5521 tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca
     5581 ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga
     5641 tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc
     5701 gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc
     5761 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg
     5821 tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc
     5881 gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac
     5941 agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg
     6001 cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga
     6061 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg
     6121 ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg
     6181 ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta
     6241 cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt
     6301 gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
     6361 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
     6421 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
     6481 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg
     6541 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
     6601 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
     6661 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
     6721 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
     6781 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
     6841 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
     6901 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
     6961 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
     7021 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
     7081 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
     7141 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
     7201 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
     7261 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac
     7321 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
     7381 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
     7441 ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc
     7501 cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc
     7561 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
     7621 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     7681 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     7741 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     7801 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     7861 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
     7921 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
     7981 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
     8041 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
     8101 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
     8161 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
     8221 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
     8281 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
     8341 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
     8401 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
     8461 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
     8521 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
     8581 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
     8641 tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
     8701 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
     8761 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
     8821 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
     8881 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
     8941 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
     9001 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
     9061 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     9121 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     9181 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     9241 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     9301 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     9361 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9421 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9481 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9541 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9601 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9961 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10021 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10081 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10141 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatg atatatctcc
    10201 caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact tgacctgata
    10261 gtttggctgt gagcaattat gtgcttagtg catctaatcg cttgagttaa cgccggcgaa
    10321 gcggcgtcgg cttgaacgaa tttctagcta gacattattt gccgactacc ttggtgatct
    10381 cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt
    10441 cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca
    10501 ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc
    10561 tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg
    10621 gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg
    10681 gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg
    10741 tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt
    10801 cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga
    10861 tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg
    10921 aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta
    10981 cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg
    11041 agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta
    11101 cctctgatag ttgagtcgat acttcggcga tcaccgcttc ccccatgatg tttaactttg
    11161 ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
    11221 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac
    11281 atgtcataac aagaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
    11341 aaggttctgg accagttgcg tgacggcagt tacgctactt gcattacagc ttacgaaccg
    11401 aacgaggctt atgtccactg ggttcgtgcc cgaat
//
