LOCUS       sequence-100456-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with constitutive bi-directional promoter, one side: 
            SfiI cloning site for GOI (contains filler DNA), other side: GFP and
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBbi-GP
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      207..216
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(274..294)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 gcttggcctg acaggccttc gaattagtga gaaggaaaga cgcaaccagg aagcccacca
       61 agagaaagag gcatttcctg aaaagattcc cctttttgga gagccctaca aggtatttac
      121 tgaacactag acattgaagt cctgatttat cacaatgttg aaccctatga tgaaacaatt
      181 cagtataatt gagttcgaaa ctcgagccat ggtggcctca gaggccagct tggggtagtt
      241 ttcacgacac cttaaatgga agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga
      301 accaagatcc aaactcaaaa agggcaaatt ccaaggagaa ttacatcaag tgccaagctg
      361 gcctaacttc agtctccacc cactcagtat ggggaaactc catcgcataa aacccctccc
      421 cccaacctaa agacgacgta ctccaaaagc tcgagaacta atcgaggtgc ctggacggcg
      481 cccggtactc cgtggagtca catgaagcga cggctgagga cggaaaggcc cttttccttt
      541 gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt cctccatttt gagctccctg
      601 cagcagggcc gggaagcggc catctttccg ctcacgcaac tggtgccgac cgggccagcc
      661 ttgccgccca gggcggggcg atacacggcg gcgcgaggcc aggcaccaga gcaggccggc
      721 cagcttgaga ctacccccgt ccgattctcg gtggccgcgc tcgcaggccc cgcctcgccg
      781 aacatgtgcg ctgggacgca cgggccccgt cgccgcccgc gg
//
