LOCUS       sequence-100459-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with constitutive bi-directional promoter, one side: 
            SfiI cloning site for GOI (contains filler DNA), other side: RFP and
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBbi-RP
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..123
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             124..723
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(124..143)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     620..640
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(823..842)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 gagcgctccg agggccgcca ccacctgttc ctgtacggca tggacgagct gtacaagggc
       61 agtggagcta ctaacttcag cctgctgaag caggctggtg acgtcgagga gaatcctggc
      121 cccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
      181 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
      241 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
      301 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
      361 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
      421 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
      481 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
      541 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
      601 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
      661 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
      721 tgattcgaag gcctgtcgtg aattcactcc tcaggtgcag gctgcctatc agaaggtggt
      781 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
      841 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
      901 tattttcatt gcaatagtgt gttggaa
//
