LOCUS       sequence-100460-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with constitutive bi-directional promoter, one side: 
            SfiI cloning site for GOI (contains filler DNA), other side: 
            hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBbi-Hyg
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta ggcaccggtt
       61 caattgccga cccctccccc caacttctcg gggactgtgg gcgatgtgcg ctctgcccac
      121 tgacgggcac cggagcctct aggcgagacc ctgtctcaca aaataaagta agcccggact
      181 gagtgcggaa aggcgggcct ggcgggtctg gtctccccat gcgggccacc agaggccctg
      241 cagccttcag tcgcttgaag gggtaatggc gcttccactc acaaacatgg cggacagagc
      301 gtgtgaacga gatgaacagc ccctcaaaaa tatggccgcc gaggctggac ggccgtgccc
      361 cagcagcacc gcctccgcgc cccacgtgat ctctcgccgg gcacagcgct gaccgcggag
      421 gtccaaccgg aagaatgtcc ggattggaca ttcggaagag ggcccgcctt ccctggggaa
      481 tctctgcgca cgcgcagaac gcttcgacca atgaaaacac aggaagccgt ccgcgcaacc
      541 gcgttgcgtc acttctgccg cccctgtttc aaggtatata gccgtagacg gaacttcgcc
      601 tttctctcgg ccttagcgcc atttttttgg gtgagtgttt tttggttcct gcgttgggat
      661 tccgtgtaca atccatagac atctgacctc ggcacttagc atcatcacag caaactaact
      721 gtagcctttc tctctttccc tgtagaaacc tctgcacctg aggccaccat gaaaaagcct
      781 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
      841 ctgatgcagc tctcggaggg cg
//
